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Diss Factsheets
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EC number: 201-375-5 | CAS number: 81-77-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline with acceptable restrictions - no GLP - reduced documentation
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 6,15-dihydroanthrazine-5,9,14,18-tetrone
- EC Number:
- 201-375-5
- EC Name:
- 6,15-dihydroanthrazine-5,9,14,18-tetrone
- Cas Number:
- 81-77-6
- Molecular formula:
- C28H14N2O4
- IUPAC Name:
- 6,15-dihydroanthrazine-5,9,14,18-tetrone
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Physical state: solid
- Analytical purity: a formulation containing approx. 87% of the dye (100% pure)
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Gassner
- Mean weight at study initiation: 150g (female) and 200 g (male)
- Fasting period before study: 15 h- 20 h before administration
ENVIRONMENTAL CONDITIONS: no data
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- CMC (carboxymethyl cellulose)
- Remarks:
- (0.5%)
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 50%
MAXIMUM DOSE VOLUME APPLIED: 10 ml/kg bw. - Doses:
- 5000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: <15 min - 5h, 1 d, 4 d, 5 - 8 d, 11 - 14 d after administration
- Frequency of weighing: at the beginning, and then after 4 d, 7 d and 13 d after administration
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Based on:
- test mat.
- Clinical signs:
- other: Faeces coloured blue 24 hours after application of the test substance. This effect was no longer evident from 48 hours till end of the observation period. The animals were all in a good general condition.
- Gross pathology:
- No abnormalities detected.
- Other findings:
- A preliminary test was performed with 1000 and ca. 5000 mg/kg bw using 2 female rats/dose. None of the animals died. No clinical sign of toxicity was reported. No abnormality was seen at gross necropsy.
Any other information on results incl. tables
Table 1: Mean weight (g)
|
|
5000 mg/kg bw |
Male animals |
At beginning |
200 |
After 4 days |
196 |
|
After 7days |
186.4 |
|
After 14 days |
235 |
|
|
|
|
Female animals |
At beginning |
150 |
After 4 days |
178 |
|
After 7days |
186.8 |
|
After 14 days |
192.6 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.