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EC number: 700-924-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013-16-04 to 2013-06-06
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1215771-98-4 (as free acid)
- IUPAC Name:
- 1215771-98-4 (as free acid)
- Details on test material:
- - Name of test material (as cited in study report): Acid Red RN 2949
- Molecular formula:
C41H30N4Na2O10S2 (as sodium salt)
C41H32N4O10S2 (as free acid)
- Molecular weight:
848.82 g/mol (as sodium salt)
804.86 g/mol (as free acid)
- Physical state: Dark red powder
- Analytical purity: 100 % (composition see AZ. No. 12-2302/4928 v1., dated 2012-08-28)
- Purity test date: 2013-02-21
- Lot/batch No.: Reg (=Batch 48)
- Expiration date of the lot/batch: 2015-03-05
- Stability under test conditions: see results
- Storage condition of test material: Room temperature, protected from light, in original container
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 25.0 - 50.0 - 100 - 200 - 400 mg/L
- Sampling method: All tested concentration levels and the control were analytically
the test item verified at the start of the exposure (0 h) and at the end of the exposure (48 h) in all concentration levels and in the control as specified below.
At the start of the exposure, sampling was carried out immediately after preparation of the test concentrations.
At the end of the exposure (48 h), samples were taken from additional replicates. The additional replicates were prepared with test media, but without daphnids, and incubated under test conditions until sampling.
- Sample storage conditions before analysis: All original samples were stored at 6 ± 2 °C. Prepared samples were stored at room temperature until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution (400 mg/L of the test item were weighed out) was freshly prepared with dilution water (0 h). Dispersion treatment was agitation.
- Eluate: Dilution water
- Differential loading: 25.0 - 50.0 - 100 - 200 - 400 mg/L
- Controls: Dilution water without test item tested under the same conditions as the test groups
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (STRAUS)
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- Lufthygiene (WaBoLu), further own breeding
- Age at study initiation (mean and range, SD): 2 to 24 h
- Method of breeding: In 2-3 L glass vessels with appr. 1.8 L culture medium, at 20 +/- 2 °C, in an incubator, 16 h illumination, illumination strength max. 20 µExm-2 x s-1
- Feeding during test: No feeding
ACCLIMATION
- Acclimation period: 2 h
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: No feeding
- Health during acclimation (any mortality observed): Healthy, no mortality observed
DILUTION WATER:
Dilution water ISO test water, according to OECD 202, Annex 3:
Component Concentration [mg/L]
KCl 5.76
NaHCO3 64.8
CaCl2 2 H2O 294
MgSO4 7 H2O 123
pH-value: 7.8 0.2
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No
Test conditions
- Hardness:
- Dilution water at test start: 256 (mg CaCO3/L)
- Test temperature:
- 19.0 °C (Water)
- pH:
- Water Quality Parameters at the Start of the Exposure (0 h)
(measured in one additional replicate per concentration level and control)
Nominal
test item concentration
[mg/L] pH-value
400 7.77
200 7.75
100 7.74
50.0 7.70
25.0 7.65
Control 7.82
Water Quality Parameters at the End of the Exposure (48 h)
(measured in all replicates)
Nominal
test item concentration
[mg/L] pH-values
Replicates
1 2 3 4
400 7.58 7.57 7.56 7.55
200 7.57 7.57 7.58 7.58
100 7.61 7.60 7.60 7.58
50.0 7.63 7.62 7.61 7.60
25.0 7.64 7.63 7.63 7.63
Control 7.63 7.69 7.71 7.72 - Dissolved oxygen:
- Water Quality Parameters at the Start of the Exposure (0 h)
(measured in one additional replicate per concentration level and control)
Nominal
test item concentration
[mg/L] Dissolved O2-concentration
[mg/L]
400 8.65
200 8.74
100 8.69
50.0 8.76
25.0 8.63
Control 9.07
Water Quality Parameters at the End of the Exposure (48 h)
(measured in all replicates)
Nominal
test item concentration
[mg/L] Dissolved O2 -concentration [mg/L]
Replicates
1 2 3 4
400 7.34 7.29 7.55 7.26
200 7.75 7.74 7.83 7.90
100 7.79 7.84 7.84 7.59
50.0 7.98 7.90 7.86 7.85
25.0 7.70 7.85 8.00 8.04
Control 8.16 8.27 8.27 8.28 - Salinity:
- Not measured, freshwater
- Nominal and measured concentrations:
- please refer to "Any other information on materials and methods"
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers, 50 mL
- Type (delete if not applicable): closed with parafilm
- Material, size, headspace, fill volume: Glass beakers (4 cm ID x 7 cm H), 50 mL
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): static test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): None
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: see above
- Conductivity: Dilution water at test start:654 µS/cm
- Culture medium different from test medium: Elendt M4 acc. to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L
- Intervals of water quality measurement: At test start and test end
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16:8 light:dark cycle
- Light intensity: Diffuse light, illumination strength max. 20 µE x m-2 x s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobility was determined in all groups after 24 h and 48 h.
TEST CONCENTRATIONS
- Range finding study:
Immobilisation Rates in the non GLP Preliminary Range Finding Test
(n = 20, divided into 2 replicates with 10 daphnids each)
Nominal
test item concentration
[mg/L] IMMOBILISATION [%]
24 h 48 h
Replicates Replicates
1 2 MV 1 2 MV
100 0 0 0 20 30 25
Control 0 0 0 0 0 0 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: (not applicable)
- Details on results:
- - Mortality of control: 0 %
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50/LC50: 1.66 (CI 1.48 - 2.01) mg/L - Reported statistics and error estimates:
- The EC10-value after 24 h was calculated by sigmoidal dose-response regression. The confidence interval for the EC10 was calculated from the best-fit values, the standard error and the t-distribution with the software GraphPad prism5.
The EC50-value for the reference item was calculated by sigmoidal dose-response regression. The confidence interval for the EC50 was calculated from the best-fit values, the standard error and the t-distribution with the software GraphPad prism5.
Any other information on results incl. tables
Immobilisation Rates after 24 and 48 h of Exposure in the Definitive Test
(n = 20, divided into 4 replicates with 5 daphnids each)
Acid Red RN 2949 |
IMMOBILISATION [%] |
|||||||||
Nominal test item concentration [mg/L] |
24 h |
48 h |
||||||||
Replicates |
Replicates |
|||||||||
1. |
2. |
3. |
4. |
MV |
1. |
2. |
3. |
4. |
MV |
|
400 |
40* |
40* |
20* |
40* |
35 |
40* |
40* |
20* |
40* |
35 |
200 |
0 |
0 |
0 |
0 |
0 |
20* |
20* |
0 |
20* |
15 |
100 |
20* |
0 |
0 |
0 |
5 |
20* |
0 |
20* |
0 |
10 |
50.0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
25.0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the nominal concentrations of the test item Acid Red RN 2949 the 48 h-EC50 for Daphnia magna was > 400 mg/L. The NOEC after 48 h was 100 mg/L, which was also the EC10 after 48 h. The LOEC after 48 h was 200 mg/L.
- Executive summary:
In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item Acid Red RN 2949 (batch number: Reg(=Batch 48)) were determined according to OECD 202 (2004) from 2013-06-04 to 2013-06-06 at Dr.U.Noack-Laboratorien, Käthe-Paulus-Str.1, 31157 Sarstedt, Germany.
The study was conducted under static conditions over a period of 48 h with 5concentrations of the test item Acid Red RN 2949 in the range of 25.0 to 400 mg/L, prepared with dilution water in a geometric series with a separation factor of 2. Twenty daphnids were exposed to each concentration level and the control.
The test item Acid Red RN 2949 was analytically verified by HPLC-DAD in samples of all test concentrations and the control at the start of the exposure (0 h) and at the end of the exposure (48 h).
The measured concentrations of the test item at the start of the exposure (0 h) were in the range of 96 to 104 % of the nominal values. At the end of the exposure (48 h), the measured concentrations of the test item were in the range of 97 to 104 % of the nominal values.The recoveries of the test item were within ± 20 % of the nominal values. This indicates that the test item was successfully maintained for the duration of the test. The effect values given are based on the nominal concentrations of the test item Acid Red RN 2949 (Table 1).
Typically physical effects were observed at the concentration levels of 100 to 400 mg/L. The test item precipitated after 24 h. The sedimented test item had an adverse effect on the buoyancy of the daphnids. The sedimented test item formed a densely layer of particles on the bottom of the test vessels and the affected daphnids were not able to swim into the water phase. Moreover, the test item stuck to the carapax of the daphnids.
The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start (0 h) and at the end of the exposure (48 h), were within the acceptable limits. The validity criteria of the test guideline were fulfilled.
Table 1: EC10-, EC50- (with Confidence Interval), EC100-Values, NOEC and LOEC
(based on the nominal concentrations of the test item)
Endpoints
Test
duration
[h]
Acid Red RN 2949
Nominal
test item concentration
[mg/L]
Confidence interval
p = 95%[mg/L]
EC10
24
326
203 – 400
48
100
Not applicable
EC50
24
> 400
Not applicable
48
> 400
Not applicable
EC100
24
> 400
Not applicable
48
> 400
Not applicable
NOEC
48
100
Not applicable
LOEC
48
200
Not applicable
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