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EC number: 407-550-4 | CAS number: 69184-17-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
- Endpoint:
- fish, juvenile growth test
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 Nov- 23 Dec 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 215 (Fish, Juvenile Growth Test)
- Version / remarks:
- Draft version, 1992
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyl O-(4-amino-3,5-dichloro-6-fluoropyridin-2-yloxy)acetate
- EC Number:
- 407-550-4
- EC Name:
- Methyl O-(4-amino-3,5-dichloro-6-fluoropyridin-2-yloxy)acetate
- Cas Number:
- 69184-17-4
- Molecular formula:
- Hill formula: C8 H7 Cl2 F N2 O3 CAS formula: C8 H7 Cl2 F N2 O3
- IUPAC Name:
- methyl 2-[(4-amino-3,5-dichloro-6-fluoropyridin-2-yl)oxy]acetate
- Details on test material:
- - Name of test material (as cited in study report): Fluroxypyr methyl ester
- Physical state: solid, grey/black powder
- Analytical purity: 93.6%
- Lot/batch No.: HJ 11160402A
- Expiration date of the lot/batch: 18 November 1995
- Stability of test concentrations: verified by chemical analysis
- Storage condition of test material: in original container at 4°C
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Water samples (around 200 mL in duplicate) were taken from solvent control and each exposure level (samples from replicate vessels pooled) on days 0, 1, 7, 14, 21 and 28. The samples were acidified by the addition of 2 mL 1M HCl prior to analysing. One sample from each pair was used in the analysis, being the remaining sample stored at 4 °C
- Each sample bottle was shaken and the sample transferred to a measuring cylinder. Methanol (20 mL) was added to the bottle and the washings transferred to the measuring cylinder which was then diluted to 250 mL with dechlorinated water. Using an AutoTrace SPE Workstation, a sub-sample (100 mL) was pumped through a solid phase extraction cartridge (Varian Bond Elut C18, 3 cc) which had been previously conditioned with methanol (10 mL) and 0.01 M hydrochloric acid (10 mL). The cartridge was washed with 5 mL 0.01 M hydrochloric acid and dried with nitrogen (10 minutes). The retained fluroxypyr methyl ester and fluroxypyr were eluted with ethyl acetate (5.0 mL) into a test tube. The eluate was evaporated to dryness (Dri-block, 50°C under nitrogen). The extracts were redissolved in acetonitrile: water (50 : 50 v/v) and, following further dilution with the same solvent as required, an aliquot of the processed sample was analysed. Duplicate procedural recoveries and a blank were analysed concurrently with the samples
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was dissolved in a solvent to give a series of stock solutions of 0.1865, 0.597, 1.865, 5.97 and 18.65 mg/mL. These stock solutions were dispensed automatically by the dosing apparatus into the flowing test water to give the desired series of exposure levels
- Controls: control and solvent control
- Chemical name of vehicle: dimethylformamide
- Concentration of vehicle in test medium: 27 µL/L test medium
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Westacre Trout Farm, Norfolk, UK
- Weight at study initiation: 1.5-2.3 g (wet)
- Acclimation: the stock of fish was obtained from the supplier on 12 October 1993 and acclimatised to test conditions from 15 - 25 November
1993. Temperature remained at 14 °C and dissolved oxygen within the range 9.5 - 10.1 mgO2/L. The fish were fed daily to repletion with commercial trout pellets throughout the acclimatisation and was withheld for 24 hours prior to the performance of any fish weighing procedures. No medication was given during the acclimatisation period and mortalities were recorded as 0 % in the 7 days prior to the test.
FEEDING DURING TEST
- Food type: commercial trout pellets
- Amount: approximately 4% of bodyweight per day
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Total exposure duration:
- 28 d
Test conditions
- Hardness:
- 166 ± 26 mgCaCO3/L
- Test temperature:
- 13 ± 1 °C
- pH:
- 7.7 ± 0.2
- Dissolved oxygen:
- 9.6-9.7 mg O2/L
- Nominal and measured concentrations:
- Nominal: control, 0.005, 0.016, 0.050, 0.16, 0.5 mg/L
Measured: control, 0.0079, 0.015, 0.048, 0.18 and 0.58 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria (25 x 46 x 25 cm)
- Aeration: no supplementary aeration was provided
- Type of flow-through: peristaltic
- Renewal rate of test solution: solvent stock solutions were dispensed at the rate of 1.6085 mL/h into a diluent stream of 1000 mL/vessel/min
- No. of organisms per vessel: 16
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate: initial loading of 1.5 g bw/L (static volume) and 0.02 g bw/L (volume in 24 h)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory tap water filtered, dechlorinated and softened by passage through a water purification system. The water passes in sequence through a high pressure sand filter to remove colloids and particulate matter and a high grade activated carbon filter to remove chlorine and any organic contaminants. A proportion of the supply then passes through a water softener before final reverse osmosis treatment to produce a highly purified water supply. The two grades of dechlorinated water are then remixed in the ratio of approximately 1: 1 to give a supply with the desired water hardness
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light/8 hours darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): growth (weight and length), mortality and sub-lethal effects at 0, 14 and 28 days
VEHICLE CONTROL PERFORMED: yes
Results and discussion
Effect concentrations
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.58 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth, mortality
- Details on results:
- - During the 28-day exposure period, no significant lethal or sub-lethal effects were evident in any test or control group. One fish was moribund from day 24 onwards at 0.58 mg/L and there was one mortality recorded ac 0.18 mg/L (nominal) on Day 15. Two fish from the solvent control group
and two from the 0.0079 mg/L group were lost between Days 14 - 15 when they jumped from their uncovered aquaria, but these incidents were not
test substance-related and did not affect the interpretation of the data. No other major adverse effeets were observed.
- Slight but significant differences between the growth rates of test and treated fish were apparent on occasions in none of these cases was there an
adverse effect attributable to treatment and no dose-response relationship was detected (the only effect observed was a slight inerease in growth rate)
Any other information on results incl. tables
Table 1. Mean bodyweight and standard length (± SD)
Mean measured concentration (mg/L) |
Day 0 |
Day 14 |
Day 28 |
|||
Weight (g) |
Length (cm) |
Weight (g) |
Length (cm) |
Weight (g) |
Length (cm) |
|
Control R1 R2 |
1.84(±0.19) 1.83(±0.24) |
4.6(±0.2) 4.6(±0.2) |
2.98(±0.50) 2.83(±0.58) |
5.5(±0.3) 5.3(±0.4) |
5.03(±0.88) 4.77(±1.13) |
6.4(±0.4) 6.4(±0.5) |
Solvent control R1 R2 R3 R4 |
2.03(±0.18) 2.05(±0.21) 1.81(±0.25) 1.94(±0.15) |
4.8(±0.2) 4.8(±0.3) 4.6(±0.3) 4.8(±0.2) |
3.27(±0.48) 3.18(±0.44) 2.87(±0.52) 3.21(±0.40) |
5.6(±0.4) 5.5(±0.3) 5.3(±0.4) 5.5(±0.3) |
5.24(±0.88) 5.32(±0.80) 4.57(±0.84) 4.92(±0.74) |
6.7(±0.4) 6.7(±0.4) 6.5(±0.5) 6.6(±0.5) |
0.0079 R1 R2 |
1.85(±0.21) 1.92(±0.26) |
4.7(±0.2) 4.8(±0.4) |
2.84(±0.49) 2.97(±0.57) |
5.3(±0.3) 5.4(±0.4) |
4.30(±1.08) 4.68(±1.06) |
6.4(±0.5) 6.6(±0.6) |
0.015 R1 R2 |
1.85(±0.23) 1.83(±0.24) |
4.8(±0.2) 4.8(±0.3) |
3.28(±0.75) 3.19(±0.64) |
5.7(±0.4) 5.5(±0.3) |
4.84(±0.77) 5.49(±1.12) |
6.7(±0.4) 6.9(±0.4) |
0.048 R1 R2 |
1.87(±0.25) 1.84(±0.25) |
4.7(±0.3) 4.8(±0.3) |
3.17(±0.68) 3.17(±0.70) |
5.6(±0.5) 5.7(±0.3) |
5.76(±1.36) 5.36(±1.33) |
7.0(±0.5) 7.0(±0.5) |
0.18 R1 R2 |
1.86(±0.24) 1.94(±0.21) |
4.8(±0.2) 4.9(±0.2) |
3.08(±0.52) 3.20(±0.69) |
5.5(-±0.5) 5.7(±0.4) |
5.41(±1.38) 5.40(±1.04) |
6.9(±0.5) 6.9(±0.4) |
0.54 R1 R2 |
1.81(±0.26) 1.89(±0.22) |
4.8(±0.3) 4.9(±0.3) |
3.00(±0.51) 3.32(±0.78) |
5.5(±0.5) 5.4(±0.4) |
5.07(±1.24) 4.91(±1.14) |
6.6(±0.5) 6.6(±0.5) |
R1 Replicate 1
R2 Replicate 2
SD Standard deviation
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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