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EC number: 931-228-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10.06.1997 - 13.08.1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- Adopted: 4 April 1984
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
- Water solubility (under test conditions): 500 mg/L
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: 6.3, 12.5, 25.0, 50.0, 100 mg/L
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Source: municipal sewage plant Sindlingen (Frankfurt/ Main)
- Suspension: 450 ml/L, after centrifugation 30.03 g/ sedimented sludge (dry mass: 3.56g/L)
- Preparation of inoculum for exposure: activated sludge was 3x centrifuged, resuspended in water containing 0.9% NaCl
- Pretreatment: not adapted
- Initial biomass concentration: 1.6 g/L (dry mass, final concentration in test volume) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- Incubation time with TS
- Hardness:
- no data
- Test temperature:
- 19.3°C - 20.4°C
- pH:
- 7.0 - 7.8 (after incubation)
- Dissolved oxygen:
- 6.0 mg/L - 8.3 mg/L at test start
- Salinity:
- no data
- Nominal and measured concentrations:
- 6.3, 12.5, 25.0, 50.0, 100 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume: 200 mL
- Aeration: 50 L/h during incubation
- No. of vessels per concentration (replicates): two
- No. of vessels per control (replicates): three
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorphenol (5, 20, and 30 mg/L)
- Duration:
- 3 h
- Dose descriptor:
- EC0
- Effect conc.:
- > 6.3 - < 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: see report table 2 (page 8)
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 33 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- 66 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 12 mg/L (C.I.: 8 mg/L - 15 mg/L, P=95 %) - Reported statistics and error estimates:
- Calculation of EC20, EC50, and EC80: Probit-Analysis (Finney 1971), Confidence interval was uncalculable
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an activated sludge, respiration inhibition test the EC50 (3 hours) was calculated to be between > 10 mg/L and 100 mg/L.
- Executive summary:
The effect of n/i-C13/C15 amine on the respiration rate of activated sludge was assessed in a GLP study according to OECD 209.
Samples of activated sludge were exposed to the test substance at nominal concentrations of 6.3, 12.5, 25.0, 50.0 and 100.0 mg/L for 3 hours. Their rates of oxygen consumption were determined and compared with those of controles, which were established at the beginning and end of the culture series.
The reference inhibitor 3,5 -dichlorophenol (3,5 -DCP) was employed in the test at 5, 20 and 30 mg/L, as a positive control. The three-hour 50% effect concentration (EC50) for 3,5 -DCP was calculated to be 12 mg/L (95% confidence limits 8 - 15 mg/L). These results show that the rest was valid and that the sample of activated sludge employed was sentitve to inhibition.
n/i-C13/C15 amine had an inhibitory effect on the respiration rate of activated sludge, resulting in an EC 50 value of > 10 mg/L - 100 mg/L.
Reference
Description of key information
In an activated sludge, respiration inhibition test the EC50 (3 hours) was calculated to be between > 10 mg/L and 100 mg/L.
Key value for chemical safety assessment
Additional information
The effect of n/i-C13/C15 amine on the respiration rate of activated sludge was assessed in a GLP study according to OECD 209.
Samples of activated sludge were exposed to the test substance at nominal concentrations of 6.3, 12.5, 25.0, 50.0 and 100.0 mg/L for 3 hours. Their rates of oxygen consumption were determined and compared with those of controles, which were established at the beginning and end of the culture series.
The reference inhibitor 3,5 -dichlorophenol (3,5 -DCP) was employed in the test at 5, 20 and 30 mg/L, as a positive control. The three-hour 50% effect concentration (EC50) for 3,5 -DCP was calculated to be 12 mg/L (95% confidence limits 8 - 15 mg/L). These results show that the rest was valid and that the sample of activated sludge employed was sentitve to inhibition.
n/i-C13/C15 amine had an inhibitory effect on the respiration rate of activated sludge, resulting in an EC 50 value of > 10 mg/L - 100 mg/L.
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