1-Propene, hydroformylation products, high-boiling

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): Oxooel 740
- Physical state: Liquid, yellowish, turbid
- Analytical purity: 2-Ethylhexane-1,3-diol: 44 area-%
- Batch No.: continuous production
- Storage condition of test material: at ambient temperatures under N2

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling method: 30 min before insertion of the test organisms (first and second interval) and at the end of the intervals

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test solutions were prepared separately for each test vessel and each interval. The test substance was homogenized by shaking. The test concentrations were stirred for 30 min to ensure that the test substance was completely dissolved.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Source: hatched at the testing facility
- Age at study initiation: ca. 3 months
- Length at study initiation (length definition, mean, range): from the top of the snout to the end of the caudal fin, 2.6 cm, 2.1- 2.9 cm
- Weight at study initiation (mean, range): 0.12 g, 0.05 - 0.16 g
- Feeding during test: no, withdrawal during the last day before start of exposure

ACCLIMATION
- Acclimation period: >= 14 days to test water, photoperiod and temperature
- Acclimation conditions: same as test
- Photoperiod: 16 hours light, 8 hours darkness
- Type and amount of food: Commercial fish diet "Tetramin", supplier Tetra-Werke, Melle, Germany
- Feeding frequency: ad libitum, additionally generally on workdays live brine shrimp (Artemia nauplii)
- Water quality: Non chlorinated charcoal filtered tap water, mixed with deionized water
- Total hardness: ca. 100 mg/L CaCO3
- Acid capacity: ca. 2.5 mM
- Oxygen content: > 80% saturation
- pH: generally 7.5 - 8.5
- Temperature: ca. 25 - 26 °C
- Mortality during the last week of exposure: > 5 %
- Medical treatment: none

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

100 mg/L CaCO3

Test temperature:

23 °C

pH:

7.9 - 8.3

Dissolved oxygen:

6.3 - 8.5 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 4.6, 10, 22, 46, 100 mg/L
Measured test concentrations (mean): -, 4.2, 9.1, 21.7, 44.2, 94.3 mg/L
Recovery rates: -, 91, 91, 99, 96, 94%

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: closed
- Material, fill volume: glass bottles covered with glass plugs, 10 L
- Aeration: none
- Renewal rate of test solution: after 48 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.08 g fish/L water

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: non chlorinated charcoal filtered tap water mixed with deionized water
- Alkalinity: ca. 2.5 mM
- Ca/Mg ratio: 4: 1
- Conductivity: ca. 250 µS/cm (25 °C)
- Culture medium different from test medium: no
- Intervals of water quality measurement: Temperature, oxygen content and pH after 1, 24, 48 (new and old solutions), 72 and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours darkness
- Light intensity: ca. 100 - 490 Lux

EFFECT PARAMETERS MEASURED: mortality and toxic signs after 1, 6, 24, 48, 72 and 96 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Range finding study: yes
- Results used to determine the conditions for the definitive study: LC50 was between 10 and 100 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

68 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Measured effect concentrations after 96 hours:
LC0 = 44 mg/L
LC50 = 64 mg/L
LC100 = 94 mg/L

- Behavioural abnormalities:
- control: none
- 4.6 mg/L: none
- 10 mg/L: none
- 22 mg/L: none
- 46 mg/L: none
- 100 mg/L: tottering (1 h and 6 h, 3 fish); apathy (6 h and 24 h, 3 fish)
- Mortality of control: none

Results with reference substance (positive control):

The median lethal concentration (LC50) were calculated using probit method based on Finney+ with SAS (version 8.2.) software.