Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 939-412-9 | CAS number: 85586-38-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Batch no.: 0016420429
- Purity: ca. 76 %
- Impurities: 17.9 % triethanolamine, 2.5 % sulfates, 2.5% Mg, 1.6% water, 0.05 % cyclohexanol
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
The stability of the test substance under storage conditions over
the test period was guaranteed by the sponsor, and the sponsor
holds this responsibility. - Analytical monitoring:
- yes
- Details on sampling:
- Samples for concentration control analysis were taken from freshly prepared solution (0 h and 24 h) in additionally prepared vessels without daphnids and at the end of each exposure interval (after approx. 24 h and 48 h) from all test vessels with mobile daphnids (pooled replicates) or from all remaining test concentrations.
Additionally, samples were collected from the 24-hour old abiotic replicate (no Daphnia) per test group. - Vehicle:
- no
- Details on test solutions:
- Each test solution was prepared separately (differential loading) by directly adding test
substance to test medium. For each concentration, the test substance was weighed into a
volumetric flask and filled to the mark with test medium. The solution was then stirred for approximately 1 hour. The solutions were checked visually to ensure that the test substance is completely dissolved. All prepared solutions were visibly clear. The control solutions were
treated in the same way.
Fresh test solutions were prepared and renewed daily - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Source: The clone of Daphnia magna STRAUS used was supplied by Institut National de Recherche Chimique AppIiquée, France, in 1978. The daphnids are cultured under standard conditions in the laboratory.
- Age at study initiation: < 24 hours
- Age of stock animals: 2 - 4 weeks
- Culture water: Synthetic M4 medium
- Feeding during test: no - Test type:
- semi-static
- Water media type:
- freshwater
- Remarks:
- Synthetic M4 medium
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.2 - 3.2 mmol/L
- Test temperature:
- 19.8 - 20.2 °C
- pH:
- 7.5 - 8-5
- Dissolved oxygen:
- 8.8 - 9.3 mg/L
- Conductivity:
- 550 - 650 µS/cm
- Nominal and measured concentrations:
- Nominal: 0, 0.22, 0.46, 1.0, 2.2, 4.6, 10 mg/L
Mean measured: 0, 0.188, 0.393, 0.855, 1.790, 3.880, 9.070 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (nominal volume 100 mL)
- Type : open
- Volume of solution: 50 mL
- Aeration: none
- Renewal rate of test solution (frequency): 24h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.1 animals/mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4
- Total organic carbon: < 2 mg/L
- pH: 7.5-8.5
- Ca/mg ratio: 4:1
- Conductivity: 550-650 µS/cm
OTHER TEST CONDITIONS
- Adjustment of pH: 8.0
- Photoperiod: day/night 16:8 hours
- Light intensity: 132-794 lux at a wave length of 400-750 nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Immobilisation after 24h and 48h
- Reference substance (positive control):
- yes
- Remarks:
- sodium chloride
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.84 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 10.011 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- EC50: 4.63 g/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance is acutely toxic to aquatic invertebrates.
Reference
Description of key information
EC50 (48h) = 3.84 mg/L (mean measured) for immobilisation of Daphnia magna (OECD 202)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 3.84 mg/L
Additional information
One study
investigating the short-term toxicity of the test substance to aquatic
invertebrates is available. The study was conducted according to OECD
202 and GLP using Daphnia magna as test organism (BASF SE,
2020). Nominal concentrations between 0.22 and 10 mg/L were tested. The
exposure was analytically verified. No immobilized daphnids were
observed in the control. After an exposure time of 48h the EC50 is
determined to be 3.84 mg/L basd on mean measured concentrations. Thus,
the test substance can be considered as acutely toxic to aquatic
invertebrates.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.