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EC number: 235-428-9 | CAS number: 12225-21-7 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 19140:1.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Justification for type of information:
- Data is from the exeprimental report followed standard gudeline.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Principles of method if other than guideline:
- Determination of the effect of Aluminium, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1H-pyrazole-3-carboxylic acid complex(CAS No12225-21-7) on the
growth of freshwater green alga Chlorella vulgaris (OECD Guideline No.201). - GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (IUPAC name): aluminum tris(4-{[3-carboxy-5-oxo-1-(4-sulfophenyl)-4,5-dihydro-1H-pyrazol-4-yl]diazenyl}benzenesulfonate)
- Common name: FD&C Yellow No. 5 Aluminum Lake
- Molecular formula: C48H33AlN12O27S6
- Molecular weight: 495.4038 g/mol
-InChl:1S/C16H12N4O9S2.Al/c21-15-13(18-17-9-1-5-11(6-2-9)30(24,25)26)14(16(22)23)19-20(15)10-3-7-12(8-4-10)31(27,28)29;/h1-8,13H,(H,22,23)(H,24,25,26)(H,27,28,29);/b18-17+;
- Substance type: Organic
- Physical state: solid - Analytical monitoring:
- no
- Details on test solutions:
- The test solution was prepared in aseptic condition. The test item Aluminium, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1H-pyrazole-3-carboxylic acid complex was prepared by adding 50 mg of test item in 250 ml of BBM to get the final concentration of 200 mg/L. This stock solution was kept for stirring/sonication for 30 minutes to obtain a homogenous solution for the experiment. The test concentrations were chosen according to the available data of the test item.
The concentrations chosen were set up to the water solubility limit. The remaining test solutions were prepared by dilution from the above stock solution. To have a
better growth and visibility of cells, the initial of the culture was kept 1 X 104cells/ml. Care was taken to have a homogeneous solution for the experiment. - Test organisms (species):
- Chlorella vulgaris
- Details on test organisms:
- The fresh water green alga Chlorella vulgaris, was used as the test organism. Sterile, unicellular, liquid cultures of algae.
The culture was examined under the microscope to confirm that it was unicellular, healthy and not contaminated.
The medium to be used for the growth of algae was Bold’s Basal Medium (BBM). It is a medium composed of macronutrients, micronutrients, alkaline EDTA solution and Iron solution. Stock solution of each of these was prepared separately and then a complete medium was prepared and sterilized. De-ionized water was used to prepare the BBM. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
- Test temperature:
- 24 °C ±2°C.
- pH:
- 6.7 to 7.4
- Nominal and measured concentrations:
- 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L,200 mg/L.All the six concentration were in geometric series spaced by a factor of 2.
- Details on test conditions:
- Light Quality White Fluoroscent Light
Light Intensity:1500Lux
Photoperiod 16 Hour Light Period : 8 Hour Dark Period
RPM Speed 120 Revolutions per minute
Water Deionized Waer
Test vessel size 100ml
Test solution volume 60ml
Dilution water Bold’s Basal Medium
Test concentration Six test concentration were:
Replicates per concentration
Three replicates for Control
Two replicates for each test concentration - Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 200 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: calculated from equation through probit analysis
- Details on results:
- The microscopic observations were also noted in each of the experimental flasks. All the cells appeared healthy, round and green throughout the test duration in the control and in the experimental flask also no significant changes were observed.
- Validity criteria fulfilled:
- yes
- Conclusions:
- After 72 hours of exposure to Aluminium, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1H-pyrazole-3-carboxylic acid complex(CAS No12225-21-7) to various nominal test concentration, EC50 calculated from equation through probit analysis was found to be >200 mg/L.
- Executive summary:
The effect of test item Aluminium, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1Hpyrazole-3-carboxylic acid complex, (CAS No. 12225-21-7) was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L,200 mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be >200 mg/L. It can be concluded from the value that the Aluminium, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1Hpyrazole-3-carboxylic acid complex, (CAS No. 12225-21-7) is not toxic to the aquatic algae and can be considered as "not classified" as per the classifiecation criteria for aquatic environment.
Reference
Table 1: Showing the average cell count using Haemocytometer of the test vessels at an
equal interval of 24hrs, 48hrs and 72hrs
Test vessels and test concentration |
24 Hours |
48 Hours |
72 Hours |
Control |
|||
Replicate1 |
12800 |
54400 |
70000 |
Replicate2 |
14800 |
55200 |
69200 |
Replicate3 |
12000 |
54800 |
67600 |
CAS No. 12225-21-7 |
|||
6.25mg/l |
|||
Replicate1 |
10800 |
49600 |
57200 |
Replicate2 |
11600 |
49200 |
60400 |
12.5mg/l |
|||
Replicate1 |
11600 |
50400 |
57600 |
Replicate2 |
12800 |
52000 |
61600 |
25mg/l |
|||
Replicate1 |
14000 |
52400 |
61600 |
Replicate2 |
13200 |
50400 |
58000 |
50mg/l |
|||
Replicate1 |
12800 |
49600 |
58400 |
Replicate2 |
12400 |
52400 |
63600 |
100mg/l |
|||
Replicate1 |
12000 |
50400 |
66400 |
Replicate2 |
14400 |
50000 |
62800 |
200mg/l |
|||
Replicate1 |
11600 |
53200 |
64800 |
Replicate2 |
11600 |
44000 |
66000 |
Table 2: Showing the values of average specific growth rate and percentage inhibition
after an interval of 72 hours
|
CONTROL |
6.25mg/l |
12.5mg/l |
25mg/l |
50mg/l |
100mg/l |
200mg/l |
|||||||
Average Specific Growth rate (μ) |
R1 |
0.648 |
R1 |
0.518 |
R1 |
0.583 |
R1 |
0.606 |
R1 |
0.588 |
R1 |
0.631 |
R1 |
0.622 |
R2 |
0.644 |
R2
|
0.599
|
R2
|
0.606
|
R2
|
0.585
|
R2
|
0.616
|
R2
|
0.612
|
R2
|
0.629
|
|
R3 |
0.637 |
|||||||||||||
|
||||||||||||||
Mean of Avg. Specific growth rate |
0.643 |
0.590 |
0.594 |
0.595 |
0.602 |
0.621 |
0.625 |
|||||||
Percentage Inhibition (%I) |
_ |
8.250 |
7.560 |
7.380 |
6.375 |
3.377 |
2.722 |
Table 3: Depicting pH values at 0 Hours and after 72 Hours of test item
exposure to algae
Test vessels and test concentration |
0 Hours |
72 Hours |
CONTROL |
||
Replicate1 |
6.84 |
6.68 |
Replicate2 |
6.75 |
6.64 |
Replicate3 |
6.75 |
6.4 |
Average |
6.78 |
6.59 |
CAS No.12225-21-7 |
||
6.25mg/l |
||
Replicate1 |
6.72 |
6.46 |
Replicate2 |
6.69 |
6.43 |
12.5mg/l |
||
Replicate1 |
6.68 |
6.38 |
Replicate2 |
6.68 |
6.36 |
25mg/l |
||
Replicate1 |
6.58 |
6.44 |
Replicate2 |
6.62 |
6.42 |
50mg/l |
||
Replicate1 |
6.64 |
6.43 |
Replicate2 |
6.68 |
6.48 |
100mg/l |
||
Replicate1 |
6.62 |
6.45 |
Replicate2 |
6.62 |
6.43 |
200mg/l |
||
Replicate1 |
6.60 |
6.43 |
Replicate2 |
6.64 |
6.42 |
Description of key information
The effect of test item Aluminium, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1Hpyrazole-3-carboxylic acid complex, (CAS No. 12225-21-7) was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L,200 mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be >200 mg/L. It can be concluded from the value that the Aluminium, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1Hpyrazole-3-carboxylic acid complex, (CAS No. 12225-21-7) is not toxic to the aquatic algae and can be considered as "not classified" as per the classifiecation criteria for aquatic environment.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 200 mg/L
Additional information
Various studies including experimental data following OECD guideline for toxicity to aquatic algae with the target substance Aluminium, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1H-pyrazole-3-carboxylic acid complex(CAS No12225-21-7) were summarized as follows:
Data is from UERL 2017, exeprimental report followed standard gudeline suggests that The effect of test item Aluminium, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1Hpyrazole-3-carboxylic acid complex, (CAS No. 12225-21-7) was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L,200 mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be >200 mg/L. It can be concluded from the value that the Aluminium, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1Hpyrazole-3-carboxylic acid complex, (CAS No. 12225-21-7) is not toxic to the aquatic algae and can be considered as "not classified" as per the classifiecation criteria for aquatic environment.
Based on another study report,whose aim of the study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 130.0 mg/l was prepared by dissolving dark yellow powder in OECD growth medium. The test solution was kept 30 min in ultrasonic bath. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. 0,2.6,5.6,12,27,59,130 mg/l concentration were used.With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.The median effective concentration (EC50) for the test substance Aluminium, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1H-pyrazole-3-carboxylic acid complex(CAS No12225-21-7), in algae was determined to be 74.2 mg/l (95% CI:64.0 - 86.0).on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 3 as per the CLP classification criteria.
Even though varied results for short term toxicity of aquatic algae were available but, based on the maximum notifiers in ECHA and the effect concentration from first experimental study report,we can consider that the test chemical is not toxic and can be classified as not classified as per the classifiecation criteria for aquatic environment.
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