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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Fatty acids, C8-16, 2-ethylhexyl esters was shown to be readily biodegradable with a biodegradation of 65 to 97 % after 30 days (OECD 301D).

Key value for chemical safety assessment

Additional information

In the key study, the ready biodegradability of Fatty acids, C8-16, 2-ethylhexyl esters was tested in an O2-consumption test according to OECD 301D (Closed bottle test) under GLP conditions (Richterich 2002). The biodegradation of 2 and 5 mg/L test material was monitored for 30 days. After 30 days, 97 and 65% of the initial test substance was degraded, respectively.

This result is supported by the fact that high biodegradation rates were observed in a marine BODIS test that is comparable to an ISO draft for a marine biodegradability test for poorly soluble substances. In this study, the marine BODIS test was compared to the original freshwater test. Only microorganisms present in the natural seawater samples were used as inoculum. After 28 days 96 % of the test material was degraded in the marine test, whereas 70 % was degraded in the original freshwater test. It was concluded, that readily biodegradable substances exhibit similar results in both tests (Molversmyr 1991).

The observation of ready biodegradabilty of Fatty acids, C8-16, 2-ethylhexyl esters is in consistence with all further members of the category for which ready biodegradability was observed.

Furthermore, the substance was also shown to be degraded under anaerobic conditions 77% degradation was determined after a 70 days incubation period (Richterich 1994).