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EC number: 500-233-2 | CAS number: 68891-29-2 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 Feb - 10 Mar 2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted 13 April 2004
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 110 mg a.i./L sampled at 0 hours and at 48 hours
- Sampling method: At the start of the test (0 hour), 5 mL new media samples were taken from the freshly prepared control and test media flasks. At the end of the test period (48 hours), 5 mL old media samples were taken from pooled control and test vessels. The samples were taken using an air displacement pipette fitted with a plastic pipette tip into 20 mL glass scintillation vials containing 5 mL of methanol.
- Sample storage conditions before analysis: no, samples were analysed on the day of receipt - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A 110 mg a.i./L test solution (equivalent to 328 mg test substance/L) was prepared by adding 164.16 mg of test substance to 500 mL of autoclaved Elendt M4 medium.
- Other relevant information: The test media and the control were checked for the tyndall effect using a Hach 2100 N turbidity meter. The turbidity measurement for the test solution was not significantly different to the measurement for the control and therefore the test solution was considered a true solution. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: cultured in 1 litre glass beakers containing 1000 mL of Elendt M4 medium. Fed daily with a concentrated suspension of Chlorella vulgaris
- Source: in-house culture setup originally from dormant eggs (ephippia) supplied by MicroBioTests Inc., Belgium
- Feeding during test : no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 19.9 – 20.0°C
- pH:
- 7.74 – 8.78
- Dissolved oxygen:
- 8.05 - 8.79 mg/L
- Nominal and measured concentrations:
- Nominal test substance concentration: 110 mg a.i./L
Analysis of the 110 mg a.i.L test concentration at 0 hours showed recoveries of between 110 – 116% of nominal for all the targeted constituents. - Details on test conditions:
- TEST SYSTEM
- Test vessel: tall form beakers
- Type (delete if not applicable): covered with clear plastic petri dish lids
- Material, size: glass, 100 mL
- Volume of solution: 50 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: sterilised Elendt M4 me
- Culture medium different from test medium: no
- Intervals of water quality measurement: at 0 and 48 h (contionous measurment of temperature)
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16-hour light: 8 hour dark cycle (fluorescent lighting)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mobility after 24 and 48 hours
RANGE-FINDING STUDY
- Test concentrations: 0.1, 1, 10 and 100 mg a.i./L
- Results used to determine the conditions for the definitive study: 0% immobilisation observed - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 110 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Remarks on result:
- other: Concentration based on eight representative constituents
- Details on results:
- - Other adverse effects control:
1 daphnid appeared lethargic at 24 hours
- Immobilisation of control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no precipitation observed - Results with reference substance (positive control):
- - Results with reference substance valid?
yes
- Dose-response test: yes
- EC50: (48 h): 1.48 mg/L (95% CL: 0.746 - 2.92 mg/L) - Reported statistics and error estimates:
- No statistical analysis was performed (limit test)
- Validity criteria fulfilled:
- yes
- Remarks:
- Please refer to table 2 at "Any other infrmation on results incl. tables"
Reference
Table 1. Toxicity to D. magna
Nominal Concentration [mg Active Ingredient/L] |
Number of Daphnia magna exposed |
Immobility at 24-hours [%] |
Immobility at 48-hours [%] |
Control |
20 |
0 |
0 |
110 |
20 |
0 |
0 |
Table 2: Validity criteria for OECD 202 (2004)
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0% |
yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
≥ 8.05 mg/L |
yes |
Table 3: Percentage Recovery of constituent from Nominal at 110 mg AI/L
(Concentrations of constituent established based on the content corrected concentrations) (%)
Constituent analysed
|
0-hour New Media
|
48-hour Old Media
|
Ammonium octyl sulfate
|
115 |
103 |
1-Octanol, ethoxylated (1 EO), sulfated, ammonium salt
|
113 |
104 |
1-Octanol, ethoxylated (2 EO), sulfated, ammonium salt
|
113 |
100 |
1-Octanol, ethoxylated (3 EO), sulfated, ammonium salt
|
116 |
101 |
Ammonium decyl sulfate
|
115 |
104 |
1-Decanol, ethoxylated (1 EO), sulfated, ammonium salt
|
114 |
99 |
1-Decanol, ethoxylated (2 EO), sulfated, ammonium salt
|
110 |
100 |
1-Decanol, ethoxylated (3 EO), sulfated, ammonium salt
|
112 |
106 |
AI = Active ingredient
Description of key information
EC50 (48 h) > 110 mg a.i./L, nominal (D. magna, OECD 202)
Key value for chemical safety assessment
Additional information
The assessment is based on the data currently available. New studies, based on the category review and the final decisions issued for some of the category substances, which are also relevant for this assessment, are currently being conducted. The hazard assessment with respect to aquatic toxicity will be updated once all ongoing studies have been finalised.
One key study investigating the acute toxicity of Alcohols, C8-10, ethoxylated, sulfates, ammonium salts to aquatic invertebrates is available. The GLP guideline study was conducted under static conditions according to OECD 202. The test was performed with limit concentration of 110 mg a.i./L. The test concentration was analytically monitored by LC-MS/MS. Recovery rates were ≥ 99% of nominal. No immobilisation was observed. The determined EC50 (48 h) > 110 mg a.i./L (nominal).
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