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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
18-03-1985 to 22-03-1985
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non GLP study. Deviations from the updated OECD-guideline (adopted 1992): Highest vehicle concentration: > 100 mg/L
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
: highest vehicle concentration: 954 mg/L
GLP compliance:
no
Analytical monitoring:
yes
Details on sampling:
- Sampling method: Samples for analysis were taken at 0 h and 96 h exposure. Approx. 50 ml of testwater were taken after intensively stirring and filled in a tared bottle containing 30 mL of tetrahydrofuran. The bottle was reweighed. The resulting sample weight was converted to volume.
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Stock solution: 100 g test substance were dissolved in DMF; 4000 mg MARLOPON AT50 and were made up to 1000 nl with DMF
- Controls: Water and Vehicle
- Chemical name of vehicle: DMF and Marlopon AT50
- Concentration of vehicle in test medium: Controls: 950 mg DMF and 4 mg Marlopon AT50 per liter water in the concentration used for the highest test concentration
- Evidence of undissolved material: A deposit was observed within 2h after mixing.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrafish
- Source: West Aquarium / 37431 Bad Lauterberg
- Length at study initiation: 25 mm (20-28 mm)
- Weight at study initiation: 0.13 g (0.09-0.18 g)
- Feeding during test: none

ACCLIMATION
- Acclimation period: 34 days

ADAPTATION
-no food 24 hours prior to exposure
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
180 mg CaCO3/L
Test temperature:
23 +- 1 °C
pH:
8.0
Dissolved oxygen:
Blank: 92 - 100 %
Vehicle: 65 - 100 %
Test concentrations: 70 - 100 %
Nominal and measured concentrations:
100 mg/L (nominal); 72 mg/L (measured)
Details on test conditions:
TEST SYSTEM
- Test vessel: Aquaria
- Material, size, headspace, fill volume: glass; 20 L (36/22/25 cm); 15 L
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.10 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water (carbon filter)
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Photoperiod: Fluorescent light, 16 h daily

EFFECT PARAMETERS MEASURED: mortality and visible abnormalities after 24, 48, 72, 96 h
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: none
- Other biological observations: none
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: yes, a deposit was observed 2h after mixing
- Effect concentrations exceeding solubility of substance in test medium: yes

Description of key information

With high probability acutely not harmful to fish. 

Key value for chemical safety assessment

Additional information

The acute toxicity to fish was assessed in a 96-h acute toxicity study to zebra-fish (Brachydanio rerio) in accordance to OECD guideline 203. The test was conducted under static conditions with a nominal concentration of 100 mg/L. The 96-h LC50 was determined to be > 100 mg/L.