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EC number: 269-323-4 | CAS number: 68223-93-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.3 (Acute Toxicity (Dermal))
- Version / remarks:
- EC 92/69/EEC (1992-07-31)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- fixed dose procedure
- Limit test:
- yes
Test material
- Reference substance name:
- Diammonium 2,2'-dithiodiacetate
- EC Number:
- 269-323-4
- EC Name:
- Diammonium 2,2'-dithiodiacetate
- Cas Number:
- 68223-93-8
- Molecular formula:
- C4H6O4S2.2H3N
- IUPAC Name:
- diammonium 2-[(carboxymethyl)disulfanyl]acetate
- Test material form:
- other: aqueous solution 48%w/w diammonium 2,2'-dithiodiacetate
- Details on test material:
- Lot# 4883
assay iodometric: 48.1% w/w
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- ten animals (5 male, 5 female) age appr. 9 weeks mean body weight 308+-8 (male), 226+-11 (female)
Acclimation 5 days before study start
Environmental condition:
- 22+-2 °C
- 30 - 70% rel. humidity
- 12/12 h light dark cycle
- ventilation: 12 cycles/h of filtered non recycled air
records of conditiones were checked daily. Instrument calibration at regular intervals.
During acclimation animals were house one to seven animals of same sex in polycarbonate cages with stainless steel lid.
During treatment period animals were housed individually in PC cages of 35.5x23.5x19.3 dimension, on autoclaved sawdust (SICSA, Alfortville France)
Food and Water: All animals had free access to pelleted diet (A04C, SAFE, Villemoisson, Epinay-sur-Orge, France) and Water filtered by FG Millipore membrane 0.22µ ad libitum.
In Life date: 12 to 26 of march 2003
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- water
- Details on dermal exposure:
- TEST SITE
- Area of exposure: a dorsal area of the skin (approximately 5 cm x 6 cm for the females and 5 cm x 7 cm for the males)
- % coverage: approximately 10%
- Type of wrap if used: hydrophilic gauze pad held in contact with the skin by means of an adhesive hypoallergenic aerated semi-occlusive dressing and a restraining bandage
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1.6 mL/kg
- Constant volume or concentration used: no - Duration of exposure:
- 24 h
- Doses:
- 2000 mg/kg of the 48% solution (960 mg/kg active ingredient)
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing:The animals were observed frequently during the hours following administration of the test itemfor detection of possible treatment-related clinical signs. Thereafter, observation of the animals was made at least once a day until day 15.
From day 2, any local cutaneous reaction was recorded.
The animals were weighed individually just before administration of the test item on day 1 and then on days 8 and 15.
The body weight gain of the treated animals was compared to that of CIT control animals with a similar initial body weight.
- Necropsy of survivors performed: yes
- Other examinations performed: Macroscopic necropsy examination:All study animals were subjected to a macroscopic examination as soon as possible after death. After opening the thoracic and abdominal cavities, a macroscopic examination of the main morgans (digestive tract, heart, kidneys, liver, lungs, pancreas, spleen and any other organs with obvious abnormalities) was performed. Preservation of tissues: No organ samples were taken.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD0
- Effect level:
- > 960 mg/kg bw
Any other information on results incl. tables
No clinical signs and no deaths were observed during the study.
A reduced body weight gain was observer in 2 mals and 2 females in da 1 to 8 and one male in day 8 to 15. The other anaimals were not affected in comparison to historical control animals.
No cutaneous reactions were observed.
No apparent abnormalities were observed at necroscopy of any animals.
The LD0 is higher than 2000 mg/kg of a 48% aqueous solution of the test material (equivalent to 960 mg/kg of active ingredient)
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- No effects could be observed up to the highest test concentration (2000 mg/kg). Therefore, the LD0 is determined to be >2000 mg/kg. According to the classification criteria (Dir 67/548/EC)GHS criteria the test item should not be classified.
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