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EC number: 617-769-9 | CAS number: 858956-08-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 Oct - 26 Nov 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Ministerium für Arbeit, Integration und Soziales des Landes Nordrhein-Westfalen, Düsseldorf, Germany
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: WWTP Odenthal, Germany (28 Oct 2019)
- Storage conditions: stored for one day at room temperature under continuous stirring with aeration before use
- Preparation of inoculum for exposure: washed twice by adding mineral medium and centrifuging for 10 min at 3500 rpm and 20 °C and decanting off the supernatant, aliquot of the wet sludge was dried in order to determine the wet weight/dry weight ratio of the sludge and to prepare a stock suspension (activated sludge) of 3 g dw/L, calculated amount of sludge, needed to achieve 300 mL of this stock suspension, was dissolved in mineral medium and then filled up to a defined end volume
- Concentration of sludge: 30 mg/L suspended solids
- Initial cell/biomass concentration: - Duration of test (contact time):
- 28 d
- Initial conc.:
- 97.4 mg/L
- Based on:
- ThOD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: According to OECD 301
- Test temperature: 22 ± 1 °C
- pH: 7.4 - 8.1
- pH adjusted: no
- Suspended solids concentration: 30 mg/L s
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: OxiTopControl System (WTW), 250 mL, automated respirometer flasks
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: before use, the inoculum was stored under continuous stirring with aeration
- Test performed in closed vessels due to significant volatility of test substance: yes
- Details of trap for CO2 and volatile organics if used: carbon dioxide was absorbed in sodium hydroxide
CONTROL AND BLANK SYSTEM
- Inoculum blank: 3
- Abiotic sterile control: 3
- Toxicity control: 1 - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 100 mg/L
- Parameter:
- % degradation (O2 consumption)
- Value:
- 4
- Sampling time:
- 28 d
- Results with reference substance:
- The reference compound sodium benzoate showed 87 % degradation after 14 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Interpretation of results:
- not readily biodegradable
Reference
Table 1: BOD values (mg O2/L) after n days
flask no. |
test item |
blank |
blank mean |
reference compound |
toxicity control |
|||||
10 |
11 |
12 |
1 |
2 |
3 |
4 |
5 |
13 |
||
code* |
a1 |
a2 |
a3 |
b1 |
b2 |
b3 |
bm |
r1 |
r2 |
t1 |
time [d] |
||||||||||
0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
1 |
4.3 |
5.7 |
5.7 |
5.7 |
5.7 |
5.7 |
5.7 |
34.2 |
37.0 |
37.0 |
2 |
8.5 |
8.5 |
7.1 |
8.5 |
8.5 |
8.5 |
8.5 |
78.3 |
76.9 |
81.2 |
3 |
11.4 |
11.4 |
8.5 |
11.4 |
12.8 |
11.4 |
11.9 |
98.3 |
91.2 |
94.0 |
4 |
12.8 |
12.8 |
11.4 |
11.4 |
12.8 |
12.8 |
12.3 |
130.0 |
125.0 |
128.0 |
5 |
14.2 |
14.2 |
11.4 |
12.8 |
12.8 |
14.2 |
13.3 |
138.0 |
134.0 |
138.0 |
6 |
14.2 |
14.2 |
12.8 |
12.8 |
14.2 |
14.2 |
13.7 |
144.0 |
140.0 |
144.0 |
7 |
18.5 |
15.7 |
15.7 |
11.4 |
11.4 |
11.4 |
11.4 |
145.0 |
141.0 |
147.0 |
8 |
21.4 |
18.5 |
15.7 |
12.8 |
12.8 |
14.2 |
13.3 |
150.0 |
144.0 |
150.0 |
9 |
21.4 |
18.5 |
17.1 |
12.8 |
12.8 |
14.2 |
13.3 |
152.0 |
148.0 |
154.0 |
10 |
22.8 |
18.5 |
17.1 |
14.2 |
14.2 |
14.2 |
14.2 |
155.0 |
150.0 |
155.0 |
11 |
22.8 |
18.5 |
18.5 |
14.2 |
14.2 |
12.8 |
13.7 |
157.0 |
152.0 |
158.0 |
12 |
22.8 |
19.9 |
19.9 |
14.2 |
14.2 |
14.2 |
14.2 |
158.0 |
155.0 |
160.0 |
13 |
22.8 |
21.4 |
18.5 |
14.2 |
15.7 |
15.7 |
15.2 |
161.0 |
155.0 |
160.0 |
14 |
22.8 |
19.9 |
18.5 |
12.8 |
14.2 |
14.2 |
13.7 |
161.0 |
157.0 |
161.0 |
15 |
24.2 |
19.9 |
18.5 |
14.2 |
15.7 |
15.7 |
15.2 |
162.0 |
160.0 |
162.0 |
16 |
24.2 |
21.4 |
18.5 |
15.7 |
15.7 |
15.7 |
15.7 |
164.0 |
162.0 |
162.0 |
17 |
24.2 |
21.4 |
21.4 |
15.7 |
15.7 |
15.7 |
15.7 |
165.0 |
164.0 |
165.0 |
18 |
25.6 |
21.4 |
21.4 |
15.7 |
15.7 |
15.7 |
15.7 |
167.0 |
165.0 |
165.0 |
19 |
25.6 |
22.8 |
19.9 |
15.7 |
15.7 |
15.7 |
15.7 |
167.0 |
165.0 |
165.0 |
20 |
24.2 |
21.4 |
21.4 |
15.7 |
15.7 |
15.7 |
15.7 |
167.0 |
167.0 |
167.0 |
21 |
25.6 |
21.4 |
19.9 |
15.7 |
15.7 |
15.7 |
15.7 |
167.0 |
168.0 |
167.0 |
22 |
25.6 |
22.8 |
22.8 |
15.7 |
17.1 |
15.7 |
16.2 |
168.0 |
170.0 |
168.0 |
23 |
25.6 |
21.4 |
21.4 |
14.2 |
15.7 |
15.7 |
15.2 |
167.0 |
170.0 |
168.0 |
24 |
25.6 |
22.8 |
22.8 |
17.1 |
17.1 |
17.1 |
17.1 |
170.0 |
170.0 |
168.0 |
25 |
25.6 |
22.8 |
22.8 |
17.1 |
17.1 |
17.1 |
17.1 |
171.0 |
171.0 |
170.0 |
26 |
27.1 |
22.8 |
22.8 |
17.1 |
17.1 |
17.1 |
17.1 |
171.0 |
171.0 |
170.0 |
27 |
27.1 |
22.8 |
21.4 |
17.1 |
17.1 |
17.1 |
17.1 |
171.0 |
172.0 |
170.0 |
28** |
23.5 |
19.2 |
19.2 |
17.1 |
17.1 |
17.1 |
17.1 |
171.0 |
174.0 |
171.0 |
*Abbreviations according to test guideline.
**The oxygen uptake by nitrification was determined. The oxygen consumed by nitrification was 3.61 mg/L. This oxygen consumption was subtracted from the respective 28 days measurements of the test item.
Table 2: Degradation of test item
Test concentration: 100 mg/L
Theoretical oxygen demand: 0.974 mgO2/mg
time [d] |
blank-corrected BOD (a –bm) [mg O2/L] |
% degradation |
% degradation mean |
||||
a1 |
a2 |
a3 |
a1 |
a2 |
a3 |
||
0 |
0.0 |
0.0 |
0.0 |
0 |
0 |
0 |
0 |
1 |
0.0 |
0.0 |
0.0 |
0 |
0 |
0 |
0 |
2 |
0.0 |
0.0 |
0.0 |
0 |
0 |
0 |
0 |
3 |
0.0 |
0.0 |
0.0 |
0 |
0 |
0 |
0 |
4 |
0.5 |
0.5 |
0.0 |
0 |
0 |
0 |
0 |
5 |
0.9 |
0.9 |
0.0 |
1 |
1 |
0 |
1 |
6 |
0.5 |
0.5 |
0.0 |
0 |
0 |
0 |
0 |
7 |
7.1 |
4.3 |
4.3 |
7 |
4 |
4 |
5 |
8 |
8.1 |
5.2 |
2.4 |
8 |
5 |
2 |
5 |
9 |
8.1 |
5.2 |
3.8 |
8 |
5 |
4 |
6 |
10 |
8.6 |
4.3 |
2.9 |
9 |
4 |
3 |
5 |
11 |
9.1 |
4.8 |
4.8 |
9 |
5 |
5 |
6 |
12 |
8.6 |
5.7 |
5.7 |
9 |
6 |
6 |
7 |
13 |
7.6 |
6.2 |
3.3 |
8 |
6 |
3 |
6 |
14 |
9.1 |
6.2 |
4.8 |
9 |
6 |
5 |
7 |
15 |
9.0 |
4.7 |
3.3 |
9 |
5 |
3 |
6 |
16 |
8.5 |
5.7 |
2.8 |
9 |
6 |
3 |
6 |
17 |
8.5 |
5.7 |
5.7 |
9 |
6 |
6 |
7 |
18 |
9.9 |
5.7 |
5.7 |
10 |
6 |
6 |
7 |
19 |
9.9 |
7.1 |
4.2 |
10 |
7 |
4 |
7 |
20 |
8.5 |
5.7 |
5.7 |
9 |
6 |
6 |
7 |
21 |
9.9 |
5.7 |
4.2 |
10 |
6 |
4 |
7 |
22 |
9.4 |
6.6 |
6.6 |
10 |
7 |
7 |
8 |
23 |
10.4 |
6.2 |
6.2 |
11 |
6 |
6 |
8 |
24 |
8.5 |
5.7 |
5.7 |
9 |
6 |
6 |
7 |
25 |
8.5 |
5.7 |
5.7 |
9 |
6 |
6 |
7 |
26 |
10.0 |
5.7 |
5.7 |
10 |
6 |
6 |
7 |
27 |
10.0 |
5.7 |
4.3 |
10 |
6 |
4 |
7 |
28** |
6.4 |
2.1 |
2.1 |
7 |
2 |
2 |
4 |
Comments: **The oxygen uptake by nitrification was determined. The oxygen consumed by nitrification was 3.61 mg/L. This oxygen consumption was subtracted from the respective 28 days measurements of the testitem.
Table 3: Oxygen uptake bynitrification ( NO3-N-/NO2-N-determination)
|
time |
NO3-N concentration [mg/L] |
oxygen concen- tration [mg/L] |
NO2-N concentration [mg/L] |
oxygen concen- tration [mg/L] |
test item |
0 h |
< 1.00 |
4.57 |
< 0.100 |
0.343 |
test item |
28 d |
1.79 |
8.18 |
< 0.100 |
0.343 |
increment (oxygen uptake) |
28 d |
|
3.61 |
|
0.0 |
Total oxygenvalue (ON) |
|
3.61 |
The BOD values of the test item after 28 days were corrected for the oxygen consumed by nitrification.
Table 4: Validity criteria for OECD 301 F
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
93% |
yes |
Percentage degradation of the reference compound reached the pass level by day 14 (≥ 60%). |
87% |
yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
56% |
yes |
The oxygen uptake of the inoculum blank is normally 20-30 mg O2/L and should not be greater than 60 mg/L in 28 days. |
17.1 mg O2/L |
yes |
Description of key information
Not readily biodegradable: 4% biodegradation after 28 d (OECD 301 F).
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
One GLP guideline study investigating the ready biodegradability of test
substance is available. The study was conducted in accordance with
OECD Guideline 301 F. A suspension of the test substance in a mineral
medium was inoculated and incubated for 28 d under aerobic conditions.
During this period, degradation was followed by continuous automated BOD
determinations. The biodegradation of the test item was 4 % (ThOD) after
28 days. The test item failed the pass level requested in test method
OECD 301 F which is a biodegradation ≥ 70 % DOC or ≥ 60 % ThOD. The test
item is, therefore, considered to be not readily biodegradable. The
reference compound sodium benzoate showed 87 % degradation after 14
days.
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