Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 240-474-8 | CAS number: 16423-68-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The stock solution was prepared by dissolving 1 gm of the test substance in 1 liters of potable water (passed through reverse osmosis system). This stock solution was used for preparing test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Animal species: Zebra Fish (Danio rerio)
Body length: Average 2.33 cm
Body weight: 0.127g (average) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- 24, 48, 72 and 96 hours
- Test temperature:
- 23.7-24.7°C
- pH:
- 7.1
- Dissolved oxygen:
- 8.3mg/l
- Nominal and measured concentrations:
- Nominal test concentrations: 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness
Water: Potable water (passed through reverse osmosis system) was used
pH: 7.1
Hardness: 53.75 (mg of CaCO3)
TDS: 382 ppm
Conductivity: 1072 (µScm-1) - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No mortality were found
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: As no effects were observed on the fishes, thus LC50 value consider to be > 100 mg/l
- Sublethal observations / clinical signs:
Table 1: Mortality and symptoms
Nominal Concentration
(mg/l)
Number of fish
Mortality (cumulated) after
3 h
6 h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
-
-
-
-
-
-
-
-
-
-
-
-
6.25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
12.5
4
4
-
-
-
-
-
-
-
-
-
-
-
-
25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
50
4
4
-
-
-
-
-
-
-
-
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
-
-
-
-
Table 2: Symptoms
Nominal Concentration
(mg/l)
Number of fish
Symptoms after
3 h
6 h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
6.25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
12.5
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
50
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
100
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
Explanation of symptoms:
A:apathy
N:Narcotic-like state
C:Swimming near water surface
M: Slow movements compared to control
NS:No abnormal symptoms
S:Surfacing behaviour
L:Loss of equilibrium
SM:Shimming movement
H1:Hyperactivity
H2:Hypo activity
B:Bending behavior
F:Fin rot
IS:Invert Swimming
SB:Swimming behaviour
RF:Respiratory Function
P:Pigmentation
Table 3: pH and oxygen content:
NominalConcentration
(mg/l)
Number of fish
pH readings after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
8.5
8.5
8.3
8.4
8.2
8.3
8.3
8.4
6.25
4
4
8.5
8.4
8.5
8.5
8.4
8.4
8.5
8.4
12.5
4
4
8.5
8.4
8.4
8.4
8.4
8.4
8.4
8.5
25
4
4
8.5
8.5
8.5
8.3
8.5
8.3
8.5
8.4
50
4
4
8.5
8.5
8.3
8.4
8.3
8.3
8.3
8.3
100
4
4
8.4
8.6
8.3
8.5
8.3
8.5
8.3
8.5
NominalConcentration
(mg/l)
Number of fish
Oxygen content (mg/L) after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
8.2
8.2
7.9
8.1
8.2
8.4
7.9
7.9
6.25
4
4
8.2
8.1
8.1
8.1
7.9
8.1
7.9
7.9
12.5
4
4
8.2
8.2
7.9
7.8
7.8
8.2
7.9
7.9
25
4
4
8.2
8.1
7.9
7.8
7.8
7.8
7.8
7.8
50
4
4
8.2
8.1
7.9
7.8
7.8
7.8
7.8
7.8
100
4
4
8.1
7.9
7.9
7.8
7.9
8.2
7.8
7.8
Table 4: Test temperature
NominalConcentration
(mg/l)
Number of fish
Temperature (°C) after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
22.6
22.5
22.8
22.7
22.6
22.6
22.8
22.5
6.25
4
4
22.5
22.2
22.7
22.6
22.4
22.3
22.4
22.2
12.5
4
4
22.4
22.4
22.6
22.6
22.4
22.3
22.3
22.3
25
4
4
22.4
22.3
22.6
22.3
22.1
22.1
22.2
22.0
50
4
4
22.4
22.3
22.3
22.4
22.3
22.1
22.1
22.1
100
4
4
22.2
22.4
22.3
22.4
22.1
22.3
22.0
22.2
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, experimental median lethal Concentrations [LC-50] of test chemical on Zebra Fish (Denio rario) after exposing for 96 hours was determined to be > 100 mg/L, as no mortality were observed at 100 mg/l.
- Executive summary:
Study was conducted to assess the effect of test chemical on the mortality of fishes. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). Test conducted on Danio rerio (previous name: Brachydanio rerio). The stock solution was prepared by dissolving 1 gm of the test substance in 1 liters of potable water (passed through reverse osmosis system). This stock solution was used for preparing test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure of test chemical to various nominal test concentrations, LC50 was calculated and the mortality was used as a key parameter for the determination of exact effect on fishes. Based on nominal concentrations, experimental median lethal Concentrations [LC-50] of test chemical on Zebra Fish (Denio rario) after exposing for 96 hours was determined to be > 100 mg/L, as no mortality were observed at 100 mg/l. Thus, it can be consider that the chemical was non hazardous and can be consider to be not classified as per the CLP classification criteria.
Reference
Description of key information
Study was conducted to assess the effect of test chemical on the mortality of fishes. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). Test conducted on Danio rerio (previous name: Brachydanio rerio). The stock solution was prepared by dissolving 1 gm of the test substance in 1 liters of potable water (passed through reverse osmosis system). This stock solution was used for preparing test concentrations of 6.25 mg/L, 12.5 mg/L, and 25 mg/L, 50 mg/L & 100 mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure of test chemical to various nominal test concentrations, LC50 was calculated and the mortality was used as a key parameter for the determination of exact effect on fishes. Based on nominal concentrations, experimental median lethal Concentrations [LC-50] of test chemical on Zebra Fish (Denio rario) after exposing for 96 hours was determined to be > 100 mg/L, as no mortality were observed at 100 mg/l. Thus, it can be consider that the chemical was nonhazardous and can be consider to be not classified as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Summarized result from the various experimental sources for the determination of effect of test chemical on the mortality of fishes are as mentioned below:
Study was conducted to assess the effect of test chemical on the mortality of fishes. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). Test conducted on Danio rerio (previous name: Brachydanio rerio). The stock solution was prepared by dissolving 1 gm of the test substance in 1 liters of potable water (passed through reverse osmosis system). This stock solution was used for preparing test concentrations of 6.25 mg/L, 12.5 mg/L, and 25 mg/L, 50 mg/L & 100 mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure of test chemical to various nominal test concentrations, LC50 was calculated and the mortality was used as a key parameter for the determination of exact effect on fishes. Based on nominal concentrations, experimental median lethal Concentrations [LC-50] of test chemical on Zebra Fish (Denio rario) after exposing for 96 hours was determined to be > 100 mg/L, as no mortality were observed at 100 mg/l. Thus, it can be consider that the chemical was nonhazardous and can be consider to be not classified as per the CLP classification criteria.
Above study further supported by the second study from peer reviewed journal. Principle of this study was to determine the effect of test chemical on the mortality rate of fishes. Gambusia affinis was used as a test organism and the study performed under the static system for 96 hours. Different test concentrations 1, 10,20,50,70,100,150 and 200 mg/l respectively were used. Fishes were collected from the pound and kept in the laboratory for at least 72 hr for the stabilization prior to their use in the tests. Fishes were starved for 24hr before test. 20 fishes were transferred to the 5 gallon glass aquaria were illuminated with fluorescent lamps giving surface light intensity of 3800 µw/cm2. Probit analysis was used to determine the effect of test chemical, whereas mortality were used as a key parameter for the LC50 calculation. After the exposure of test chemical for 96 hrs with fishes Gambusia affinis, the LC50 value was determine to be > 200 mg/l on the basis of mortality observations.
Short term toxicity test was performed on Himedaka (Oryzias latipes). All fishes acclimated for 10 days in tap water before experiment. In lab, 1 liter solution having pH 7 containing 3,000 mg/liter of test chemical to be tested. 10 tested fish were kept in the tank without direct sunlight for 48 hrs and survival rate was determined. It was observed that at the 3000 mg/liter concentration, the survival rate of fish was observed to be 0 % and thus the LC100 was consider to be at 3000 mg/l.
Similarly an acute study was conducted to determine the effect of test chemical on the fishes. Test conducted in accordance with the Japanese Industrial Standards Committee. Himedaka commonly known as Oryzias latipes of same age i.e. 2 cm long and 0.2 gram in weight was used for the study. The test fishes were acclimatized for 10 days in tap water. Ten fish of Himedaka per one trial were kept in 2 liter of deionized water at 25°C and, after 24 and 48 hrs, lethal concentration of 50% fish was determined. Based on the mortality of fishes Oryzias latipes due to the test chemical exposure for 24 and 48 hours, the LC50 value was determine to be 710 and 340 mg/l respectively.
The fifth study from peer reviewed journal was conducted on Oryzias latipes was carried out for 48 hrs and TLm value was determined. Himedaka (Oryzias latipes) of same age i.e. 2 cm long and 0.2 in weight was used for the study. The test fishes were acclimatized for 10 days in tap water. In 1 liter solution of pH 7.0 containing test substance, ten fishes were added in the tank without direct sunlight for 48 hrs and survival rate was determined. Water temperature was 25°C and aeration was provided with bubbler. Median Tolerance Limit (TLm) was determined after 48 hrs. Based on the mortality of test fishes, the 48 hrs LC50 value was determine to be 900 mg/l.
Thus, on the basis of above all studies and effects observations on fishes, test chemical consider to be nontoxic and not classified as per the CLP classification criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.