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Diss Factsheets
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EC number: 212-742-4 | CAS number: 865-49-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute lethal toxicity of hydrocarbons and chlorinated hydrocarbons to two plankton crustaceans: the key role of organism-water partitioning
- Author:
- Abernethy S, Bobra AM, Shiu WY, Wells PG, Mackay D
- Year:
- 1 986
- Bibliographic source:
- Aquatic Toxicology 8, 163-174
Materials and methods
- Principles of method if other than guideline:
- Toxicity test according to Bobra et al. (1983), Chemosphere 12, 1137-1149.
- GLP compliance:
- no
Test material
- Reference substance name:
- Chloroform
- EC Number:
- 200-663-8
- EC Name:
- Chloroform
- Cas Number:
- 67-66-3
- Molecular formula:
- CHCl3
- IUPAC Name:
- chloroform
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Saturated aqueous solutions of chloroform were prepared and diluted to provide at least 5 exposure concentrations plus a control. Test chambers were filled and sealed in glass vials with teflon-lined screw caps. Air spaces in exposure chambers were eliminated to minimise volatilisation loss of the toxicant from water.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- 4 to 6 day old Daphnia from laboratory-breeded stocks were used for the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 23 +/- 2 °C
- Dissolved oxygen:
- Concentration was typically at 8 to 9 mg/L and after 48 hours, the lowest measured concentration was 5 mg/L.
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 79 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality
Applicant's summary and conclusion
- Executive summary:
The acute toxicity of chloroform to 4 to 6 day old Daphnia magna was tested in a static test with sealed vessels. The endpoint for the toxicity assessment was mortality. The LC50 value determined was 79 mg/L.
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