Sodium 3-nitrobenzenesulphonate

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from authoritative databases and secondary source

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

Qualifier:

according to guideline

Guideline:

other: "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"

Principles of method if other than guideline:

Bioaccumulation study was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical. The study was performed according to OECD Guideline 305 C "Bioaccumulation: Test for the Degree of Bioconcentration in Fish" and other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively.

GLP compliance:

not specified

Specific details on test material used for the study:

Purity: 97.8%
- Water solubility: 27.7 g/100 g (20°C)

Radiolabelling:

not specified

Vehicle:

not specified

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Length at study initiation (lenght definition, mean, range and SD): 8
- Sex: MF

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

6 wk

Test temperature:

25°C

pH:

6.0-8.5

Details on test conditions:

RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations: TLm(48h) > 1000 mg/L, Rice fish (Oryzias latipes)

Nominal and measured concentrations:

Nominal concentrations:
1st concentration area: 1 mg/l (1 ppm (w/v))
2nd concentration area: 0.1 mg/l (0.1 ppm (w/v))

Reference substance (positive control):

not specified

Key result

Conc. / dose:

1 mg/L

Temp.:

25 °C

Type:

BCF

Value:

> 0.5 - < 1.6 L/kg

Basis:

whole body w.w.

Time of plateau:

6 wk

Remarks on result:

other: pH ranges from 6-8.5, respectively

Key result

Conc. / dose:

0.1 mg/L

Temp.:

25 °C

Type:

BCF

Value:

<= 5 L/kg

Basis:

whole body w.w.

Time of plateau:

6 wk

Remarks on result:

other: pH ranges from 6-8.5, respectively

Bioaccumulation test results:

 

		2 W	3 W	4 W	6 W
1st Concentration area	Test substance concentration (ppm (w/v))	0.943	0.908	0.869	0.859
	Bioconcentration factor (L/kg)	<= 0.5	<= 0.5	<= 0.5	<= 0.5
		<= 0.5	<= 0.5	<= 0.5	<= 0.5
2nd Concentration area	Test substance concentration (ppm (w/v))	0.087	0.085	0.088	0.085
	Bioconcentration factor (L/kg)	<= 5	<= 5	<= 5	<= 5
		<= 5	<= 5	<= 5	<= 5

Validity criteria fulfilled:

not specified

Conclusions:

The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be in the range of 0.5-1.6 L/Kg at a conc. of 1 mg/l and ≤ 5 L/Kg at a conc. of 0.1 mg/l, respectively.

Executive summary:

Bioaccumulation study was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical.The study was performed according to OECD Guideline 305 C "Bioaccumulation: Test for the Degree of Bioconcentration in Fish" andother guideline"Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively under flow through conditions at a temperature of25°C and pH range 6.0-8.5, respectively.Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 1 mg/l and 0.1 mg/l, respectively. Range finding study involve theTLm(48h) > 1000 mg/LonRice fish(Oryzias latipes).The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be in the range of 0.5-1.6 L/Kg at a conc. of 1 mg/l and ≤ 5 L/Kg at a conc. of 0.1 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.

Description of key information

Bioaccumulation study from authoritative database (J-CHECK & BCF database, 2017 and EnviChem, 2014) and secondary source (IUCLID dataset, 2000) was conducted on test organism Cyprinus carpiofor 6 weeks for evaluating the bioconcentration factor (BCF value) of Sodium 3-nitrobenzene sulphonate(CAS no. 127-68-4).The study was performed according to OECD Guideline 305 C "Bioaccumulation: Test for the Degree of Bioconcentration in Fish" andother guideline"Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively under flow through conditions at a temperature of25°C and pH range 6.0-8.5, respectively.Cyprinus carpio (length - 8 dimensionless) was used as a test organism for the study. Test chemical nominal conc. used for the study were 1 mg/land 0.1 mg/l, respectively. Range finding study involve theTLm(48h) > 1000 mg/Lon Rice fish (Oryzias latipes).The bioconcentration factor (BCF value) of substance Sodium 3 -nitrobenzene sulphonate on Cyprinus carpio was determined to be in the range of 0.5-1.6 L/Kg at a conc. of 1 mg/l and ≤ 5 L/Kg at a conc. of 0.1 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the chemical Sodium 3-nitrobenzenesulphonate is not expected to bioaccumulate in the food chain.

Key value for chemical safety assessment

BCF (aquatic species):

0.5 L/kg ww

Additional information

Experimental study and predicted data for the target chemical and various supporting studies for its structurally similar read across substances were reviewed for the bioaccumulation end points which are summarized as below:

 In an experimental key study from authoritative database (J-CHECK & BCF database, 2017 and EnviChem, 2014) and secondary source (IUCLID dataset, 2000) for the target chemical, bioaccumulation experiment was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical .The study was performed according to OECD Guideline 305 C "Bioaccumulation: Test for the Degree of Bioconcentration in Fish" and other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively under flow through conditions at a temperature of25°C and pH range 6.0-8.5, respectively. Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 1 mg/land 0.1 mg/l. Range finding study involve the TLm(48h) > 1000 mg/L on Rice fish(Oryzias latipes).The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be in the range of 0.5-1.6 L/Kg at a conc. of 1 mg/l and ≤ 5 L/Kg at a conc. of 0.1 mg/l, respectively.

From CompTox Chemistry Dashboard using OPERA (OPEn (quantitative) structure-activity Relationship Application)  V1.02 model in which calculation based on PaDEL descriptors (calculate molecular descriptors and fingerprints of chemical), the bioaccumulation i.e BCF for test chemical was estimated to be 5.05 dimensionless . The predicted BCF result was based on the 5 OECD principles.

In a next weight of evidence study from authoritative database (J-CHECK, 2017) for the test chemical, bioaccumulation experiment was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical. The study was performed according to OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish) and other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively. Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 0.5mg/land 0.05 mg/l, respectively. Analytical method involve the recovery ratio: Test water: Fish: 91.0 %, - Limit of quantitation: Test water: 1st concentration area : 0.018 mg/L, 2nd concentration area : 0.0018 mg/L, Fish : 0.20 microg/g. Range finding study involve theLC50 (48h) 326 mg/L on Rice fish (Oryzias latipes). Lipid content of the test organism Cyprinus carpio was determined to be 4.1% at the start of exposure. The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be ≤ 0.4 L/Kg at a conc. of 0.5 mg/l and ≤ 4.1 L/Kg at a conc. of 0.05 mg/l, respectively.

In another weight of evidence study for test chemical, the bioaccumulation study was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical (J-CHECK, 2017). Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 1mg/land 0.1 mg/l, respectively. Range finding study involves the TLm (48 hr) ˃ 1000 mg/l (w/v) on Rice fish (Oryzias latipes). The bioconcentration factor (BCF value) test chemical on Cyprinus carpio was determined to be in the range of 0.2-0.5 L/Kg at a conc. of 1 mg/l and ≤ 2 L/Kg at a conc. of 0.1 mg/l, respectively.

On the basis of above results for target chemical (from authoritative J-CHECK, BCF database, EnviChem, secondary source & modelling database CompTox Chemistry Dashboard,2017) it can be concluded that the BCF value of test chemical ranges from 0.5–5.05 which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.