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Diss Factsheets
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EC number: 202-617-2 | CAS number: 97-90-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30-10-1989 to 08-11-1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Remarks:
- equals OECD 209, GLP.
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- - Sampling method: samples of activated sludge were withdrawn from a sewage plant mainly fed with domestic sewage
- Activated sludge was aerated during transportation to the laboratory
- Concentration(suspended solids): adjusted to 3g/l
- Temp: 20 ±2 °C
- pH: 6-8 (adjustment was not necessary) - Details on test solutions:
- - Controls one blanc control with inoculum but without test material, one physico-chemical control (with test - respectively reference- substance,
but without activated sludge, to determine the chemical oxygen consumption) - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 180 min
- Remarks on exposure duration:
- Determination of the EC50 after 30 min exposure.
- Test temperature:
- 20 ± 2°C
- pH:
- 6.0 - 8.0
- Nominal and measured concentrations:
- 100, 300, 700, 1000, and 3500 mg/l
- Details on test conditions:
- Incubation period: 30 or 180 min
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 570 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: based on oxygen consumption, graphical determination
- Key result
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- 570 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: based on oxygen consumption, graphical determination
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: based on oxygen consumption
- Duration:
- 180 min
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: based on oxygen consumption
- Results with reference substance (positive control):
- - Results with reference substance valid?: Yes
- Validity criteria fulfilled:
- yes
- Remarks:
- The EC50 of 3,5-Dichlorphenol was in the accepted range of 5 to 30 mg/L.
- Conclusions:
- In a valid respiration inhibition test according (Pseudomonas putida) to ISO 8192 (equivalent to OECD 209) after a contact time of 180 minutes, the EC50 was determined to be 570 mg/L (graphical determination).
- Executive summary:
In a valid respiration inhibition test (Pseudomonas putida) according to ISO 8192 (equivalent to OECD 209) after a contact time of 180 minutes, the EC50 was determined to be 570 mg/L (graphical determination).
NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.
Reference
The oxygen consumption (mg/l x h) was calculated from the linear part of the curves of the oxygen concentration versus time.
The percentage inhibition was calculated at each concentration as follows:
I(%) =[1- (Rs - Rpc)/ Rc)]x100
I = percentage inhibition of oxygen consumption
Rs = oxygene consumption rate by the test mixture
Rc = oxygen consumption rate by the blanc control
Rpc= oxygen uptake rate by the physico-chemical control
Table 2: Oxygen uptake and inhibition (%) of the test substance Ethyleneglycol dimethacrylate after a contact time of 30 min and 180 min
O2 uptake (mg/l*h) 30 min | Inhibition (%) 30 min | O2 uptake (mg/l*h) 180 min | Inhibition (%) 180 min | |
Control C | 45.60 | 73.71 | ||
1 mg/l | 45.12 | 1 | 67.20 | 9 |
10 mg/l | 47.40 | -4 | 63.94 | 13 |
100 mg/l | 45.48 | 0 | 67.50 | 8 |
1000 mg/l | 10.47 | 77 | 9.92 | 87 |
10000 mg/l | 5.93 | 87 | 4.61 | 94 |
phsico-chemical control | 0.00 | 0.00 |
O2 uptake (mg/l*h) 30 min | Inhibition (%) 30 min | O2 uptake (mg/l*h) 180 min | Inhibition (%) 180 min | |
Control C | 41.10 | 82.32 | ||
100 mg/l | 38.33 | 7 | 75.60 | 8 |
350 mg/l | 30.80 | 25 | 61.20 | 26 |
700 mg/l | 15.53 | 62 | 36.25 | 56 |
1000 mg/l | 9.13 | 78 | 18.13 | 78 |
3500 mg/l | 5.40 | 87 | 8.53 | 90 |
Description of key information
The toxicity of the substance to microorganisms (Pseudomonas putida) was evaluated in an activated sludge respiration inhibition test
similar to OECD guideline 209 (Klimisch score 1).The 3h-EC50 was determined to be 570 mg/l for Ethyleneglycol dimethacrylate.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 570 mg/L
Additional information
The toxicity of the substance to microorganisms (Pseudomonas putida) was evaluated in an activated sludge respiration inhibition test
similar to OECD guideline 209 (Klimisch score 1).The 3h-EC50 was determined to be 570 mg/l for Ethyleneglycol dimethacrylate.
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