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EC number: 202-617-2 | CAS number: 97-90-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- OECD 202, GLP, analytical monitoring.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- (April 1984)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Version / remarks:
- April 1996
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 31st July 1992
- Deviations:
- no
- Principles of method if other than guideline:
- Acute Immobilization Test to Daphnia magna STRAUS to determine the effects of the saturated solution of the test item as a limit concentration after
24 and 48 hours of exposure under static conditions.
The endpoint measured was the EC50 (Median Effective Concentration), a statistically derived concentration which was expected to cause immobility in 50% of the animals. An animal is defined as immobile when it is incapable of swimming (no body movement) within 15 seconds after gentle agitation of the test container. - GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Ethylene Glycol Dimethacrylate (EGDMA; CAS RN: 97-90-5)
- Analytical monitoring:
- yes
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: (STRAUS (Clone 5)
- Source: CIT
- Age at study initiation (mean and range, SD): 6 to 24 hours
- Length at study initiation (length definition, mean, range and SD): no data
- Weight at study initiation (mean and range, SD): mean weight: no data
- Method of breeding: Laboratory bred
- Feeding during test: daphnids were not fed during the test
- Food type: Scenedesmus subspicatus cultutred at CIT
- Amount: between 0.1 and 0.2 mg of carbon per Daphnia per day with a diet of Scenedesmus subspicatus
- Frequency: at least 5 times per week
ACCLIMATION
- Acclimation period: at least 2 hours in dilution water
- Acclimation conditions (same as test or not): in dilution water (ISO test water, according to OECD 202, Annex 3) of the same quality as used for
the test
- Health during acclimation (any mortality observed): no data - Test type:
- static
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 238 mg/L as CaCO3 (the same value was obtained at T0 and T48 hours in the control)
- Test temperature:
- 19.9 to 21.3 °C
- pH:
- 7.85 to 8.08
- Dissolved oxygen:
- 8.5 to 9.2 mg/L (>= 60% of the air saturation value)
- Nominal and measured concentrations:
- Preliminary test:
0, 0.025, 0.25, 2.5, 25 and 100 mg/L (nominal)
Main test:
0, 13.2, 19.8, 29.6, 44.4, 66.7 and 100 mg/L - Details on test conditions:
- TEST SYSTEM
The test method was adapted to volatile substances.
- Test vessel: closed system; test vessels were sealed and headspace kept at the minimum.
- Type (delete if not applicable):closed
- Aeration: Forced aeration was not used during the test. Test medium was aerated prior to the addition of the test item.
- No. of organisms per vessel: 20 daphnids were tested per concentration (5 per replicate).
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment before addition of animals because the pH range of all test solutions remained within the range of 6.0 to 9.0 after preparation.
- Photoperiod: 16 hours light and 8 hours dark
- Light intensity: no data
TEST CONCENTRATIONS
- Limit test concentration: 0, 100 mg/L (nominal); Results: 0% immobilisation in the control and 100% immobilisation in the limit test solution
- Range finding study
- Test concentrations. 0.025, 0.25, 2.5, 25 and 100 mg/L (nominal); Results: immobilisation was 0%, 0%, 0% and 5% , respectively
- Results used to determine the conditions for the definitive study: all results of the preiminary limit tests and the range-finding test were used. - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 19.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 44.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 39.1 - 51.6 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- concentration resulting in 100% immobilisation
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 13.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The 24-hour EC50 was not calculated by computer as the spread of immobilization across the concentrations was not sufficient for the software to undertake the calculation. A concentration producing 100% immobilization was not obtained for calculation of this EC50. The 48-hour EC50 was
calculated according to Probit analysis and the confidence interval limits according to Fieller's method. - Results with reference substance (positive control):
- No reference substance used.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this valid guideline study according to OECD 202 (static conditions) the 48 hr EC50 for Daphnia magna (STRAUS Clone 5) of Ethylene glycol dimethacrylate was 44.8 mg/L .
- Executive summary:
The 48–hr-acute toxicity of Ethylene glycol dimethacrylate to Daphnia magna straus was studied under static conditions according to OECD 202. Daphnids were exposed to control, 13.2, 19.8, 29.6, 44.4, 66.7 and 100 mg/L (nominal) of the test chemical for 48 hr.
The concentrations of Ethylene glycol dimethacrylate were analysed by HPLC with UV detection at 200 nm. The recoveries of Ethylene glycol dimethacrylate in the test solutions were determined to be within the acceptable limits.
The water quality parameters (pH and dissolveed oxygen) were measured at 0 and 48 hours and determined to be within the acceptable limits.
The 48– hour EC50was detemined to be 44.9 mg a.i./L. The 48– hr NOEC based on immobilization/sublethal adverse effects was determined to be 13.2 mg a.i./L.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results Synopsis
Test Organism Age: Daphnia magna Straus, less than 24 hours old
Test Type: static, no aeration during the test period
NOEL: 13.2 mg a.i./L
EC50: 44.9 mg a.i./L 95% C.I.: 39.1 - 51.6 mg a.i./L
Endpoint(s) Effected: immobilisation
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Reference
Immobilization Rates in the Definitive Test after 24 and 48 h of Exposure (n = 20, divided into 4 replicates with 5 daphnids each)
Measured
Concentrations in the test solutions were within +/- 20% of the nominal
values throughout the test and the control sample from time 0 confirmed
that the test item concentration was below the limit of detection. Therefore,
toxicity results were based on nominal concentrations.
Nominal Mean
Measured Concentration (mg/l)
Concentration
(mg/l) 0
Hour 24
Hours 48
Hours
0 BLD (nc) ns ns
13.2 12.8 (97) 12.4 (94) 12.1 (92)
19.8 19.3 (97) 18.9 (95) 18.3 (93)
29.6 28.6 (97) 27.2 (92) 27.1 (92)
44.4 43.9 (99) 42.4 (96) 41.8 (94)
66.7 64.2 (96) 62.7 (94) 61.1 (92)
100 96.1 (96) 94.9 (95) 93.0 (93)
(#) represent percentages of the nominal concentration
BLD = Below Limit of Detection
nc: not calculated
ns: not sampled
|
24 h |
48 h |
||||||||
|
Replicates |
Replicates |
||||||||
|
1 |
2 |
3 |
4 |
MV |
1 |
2 |
3 |
4 |
MV |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Ethylene glycol dimethacrylate 0 13.2 mg/L 19.8 mg/L 29.6 mg/L 44.4 mg/L 66.7 mg/L 100 mg/L |
0 0 0 0 0 0 5 |
0 0 0 0 0 0 4 |
0 0 0 0 0 0 5 |
0 0 0 0 0 0 4 |
0 0 0 0 0 0 4.5 |
0 0 0 0 3 4 5 |
0 0 0 1 1 4 5 |
0 0 1 1 3 5 5 |
0 0 0 1 1 4 5 |
0 0 0.25 0.75 2.0 4.25 5.00 |
MV = Mean value
The
highest concentration resulting in no immobilization (NOEC) at 48 hours
was 13.2 mg/l.
The lowest concentration resulting in 100% immobilization at 48 hours
was 100
mg/l.
The EC50 at each of the observation times was as follows:
Time (h) EC50
(mg/l) 95%
confidence limits (mg/l)
24 between
66.7 and 100 NA
48 44.9 39.1
- 51.6
NA: Not applicable
Description of key information
The acute toxicity of the substance was tested in a 48-h exposure test in Daphnia magna according to OECD guideline 202.
The 48h-EC50 was found to be 44.9 mg/l..
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 44.9 mg/L
Additional information
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