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Diss Factsheets
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EC number: 905-474-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- calculation (if not (Q)SAR)
- Remarks:
- estimated by calculation
- Adequacy of study:
- key study
- Study period:
- 2014-05-26 to 2014-05-28
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- accepted calculation method
- Remarks:
- Calculation method is used; calculation method applicable for the endpoint.
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- Calculation method
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- yes
- Remarks:
- Calculation method
- Principles of method if other than guideline:
- The acute toxicity to fish was determined using a calculation method for the Mode of Action in question. This algorithm is based on a QSAR model which has been validated to be compliant with the OECD recommendations for QSAR modeling (OECD, 2004). Further to this the lethal loading rate of the WAF is determined by using a series of calculation steps using phase equilibrium thermodynamics and excluding the non-bioavailable fraction, this approach is based on validated data derived from 96-hour LL50 tests on fish, for which the concentrations of the test item had been determined by chemical analyses over the test period.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on sampling:
- Not applicable
- Vehicle:
- no
- Details on test solutions:
- Not applicable
- Test organisms (species):
- other: Results from the following species were used in the regression: Danio rerio, Oncorhynchus mykiss, Lepomis macrochirus, Pimephales promelas, Oryzias latipes, Leuciscus idus
- Details on test organisms:
- Not applicable
- Test type:
- other: calculation method
- Water media type:
- not specified
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- LL50 (lethal loading rate of WAF)
- Post exposure observation period:
- Not applicable
- Hardness:
- Hardness is not a necessary component of the WAF calculation
- Test temperature:
- The Temperature is not a necessary component of the WAF calculation but extremely low or high temperatures could influence the solubility of certain constituents. Therefore, the calculation method is considered acceptable to determine LL50s for fish between 12 and 28 °C.
- pH:
- The pH is not a necessary component of the WAF calculation
- Dissolved oxygen:
- The oxygen concentration is not a necessary component of the WAF calculation
- Salinity:
- Salinity is not a necessary component of the WAF calculation. However as the fish QSAR for the constituents calculation was based on data from freshwater studies, the resulting calculation is considered valid for freshwater fish
- Nominal and measured concentrations:
- The calculation determines measured concentrations
- Details on test conditions:
- Calculation method
- Reference substance (positive control):
- not required
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- 6.7 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Not applicable
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Not applicable
- Sublethal observations / clinical signs:
Table 6.1.1/2: Expected concentrations at this 96 h-LL50
Constituents
concentration in the WAF (mg/L)
limonene
2.41
α-terpinene
1.20
γ-terpinene
0.70
terpinolene
0.84
isoterpinolene
0.30
para-cymene
0.14
camphene
0.31
α-phellandrene
0.04
α-pinene
0.03
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 h-LL50 for dipentene multiconstituent is 6.7 mg/L.
- Executive summary:
A calculation method prediction was performed to assess the acute toxicity of test item dipentene multiconstituent, a multiconstituent substance, to fish using the WAF method. This calculation method predicts the endpoint value which would be expected when testing the substance under experimental conditions in a laboratory following Guideline OECD 203 and EU method C.1 adapted for testing as a mixture using the WAF method. The criterion measured was the lethal loading rate of the mixture LL50 (Median Lethal Loading), a statistically derived loading rate which is expected to cause mortality in 50 % of test animals within a period of 96 h.
The acute toxicity to fish was determined using a calculation method for the Mode of Action in question. This algorithm is based on a QSAR model which has been validated to be compliant with the OECD recommendations for QSAR modeling (OECD, 2004). Further to this the lethal loading rate of the WAF is determined by using a series of calculation steps using phase equilibrium thermodynamics and excluding the non-bioavailable fraction, this approach is based on validated data derived from 96 h LL50 tests on fish, for which the concentrations of the test item had been determined by chemical analyses over the test period.
The iSafeRat® mixture module first uses Phase Equilibrium Thermodynamics to determine the analytically measurable concentrations in aqueous solutions expected from known WAF loading rates of characterised mixtures for fish, daphnids or algae. These loading rates are then divided into two parts: the bioavailable and non-bioavailable fractions per constituent. Existing experimental ecotoxicity endpoint values or predictions of these (from the single constituent ecotoxicity modules) are then used to determine the mixture ecotoxicity based on the sum of the chemical activities of the remaining bioavailable fractions of the individual constituents. The resulting value is converted back to the equivalent loading rate providing a LL50 value.
The LC50s of each constituent are already known from literature or predicted using the iSafeRat QSAR model. QMRF and QPRF are available and attached in the endpoint study record. An additivity approach (based on Chemical Activity of each constituent) is used in order to calculate the Lethal Loading of the WAF.
Using this approach, the 96-h LL50 for dipentene multiconstituent to fish is 6.7 mg/L. Based on the results of this study, dipentene multiconstituent would not be classified as acute 1 to aquatic organisms in accordance with CLP regulation (EC) No. 1272/2008.
This toxicity study is acceptable and can be used for that endpoint.
Reference
Description of key information
Using a calculation method to predict the acute toxicity of the registered substance, REACTION MASS OF DL-LIMONENE, ALPHA- GAMMA-TERPINENES, TERPINOLENE, to fish, the 96h-LL50 was found to be 6.7 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 6.7 mg/L
Additional information
A calculation method prediction was available and it has been performed to assess the acute toxicity of test item dipentene multiconstitent, a multiconstituent substance, to fish, using the WAF method. This calculation method predicts the endpoint value which would be expected when testing the substance under experimental conditions in a laboratory following Guideline OECD 203 and EU method C.1 adapted for testing as a mixture using the WAF method. The criterion measured was the lethal loading rate of the mixture LL50 (Median Lethal Loading), a statistically derived loading rate which is expected to cause mortality in 50% of test animals within a period of 96 h.
The algorithm used for the purpose of this study is based on a QSAR model which has been validated to be compliant with the OECD recommendations for QSAR modeling (OECD, 2004).
The 96h-LC50s of each constituent have been predicted using the iSafeRat QSAR model and the QMRF/QPRF have been attached to the dossier:
- p-cymene = 1.63 mg test item/L with 95 % - Confidence Limit of 1.21-2.20 mg test item/L.
- γ-terpinene = 1.09 mg test item/L with 95 % - Confidence Limit of 0.91 - 2.07 mg test item/L.
- α-phellandrene = 0.63 mg test item/L with 95 % - Confidence Limit of 0.43 - 0.92 mg test item/L.
- α-terpinene = 0.63 mg test item/L with 95 % - Confidence Limit of 0.43 - 0.92 mg test item/L.
- Isoterpinolene = 0.63 mg test item/L with 95 % - Confidence Limit of 0.43 - 0.92 mg test item/L.
- α-pinene = 0.44 mg test item/L with 95 % - Confidence Limit of 0.29 - 0.66 mg test item/L.
- Terpinolene = 1.09 mg test item/L with 95 % - Confidence Limit of 0.78 - 1.51 mg test item/L.
- Camphene = 0.44 mg test item/L with 95 % - Confidence Limit of 0.29 - 0.66 mg test item/L.
- Limonene = 1.09 mg test item/L with 95 % - Confidence Limit of 0.78 - 1.51 mg test item/L.
An additivity approach (based on Chemical Activity of each constituent) was used in order to calculate the Lethal Loading of the WAF. Using this approach, the 96-h LL50 for dipentene multiconstituent to fish is 6.7 mg/L. Based on the results of this study, dipentene multiconstituent would not be classified as acute 1 to aquatic organisms in accordance with CLP regulation (EC) No. 1272/2008. This toxicity study is acceptable and can be used for that endpoint.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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