Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 269-122-1 | CAS number: 68187-75-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 20-OCT-2009 to 05-FEB-2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: the study was performed according to OECD guideline and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Oils, fish, oxidized
- EC Number:
- 269-122-1
- EC Name:
- Oils, fish, oxidized
- Cas Number:
- 68187-75-7
- Molecular formula:
- Not necessary
- IUPAC Name:
- Triglycerides C12-24, even, saturated and unsaturated, oxidized
- Details on test material:
- - Name of test material (as cited in study report): Triglycerides, C12-24, saturated and unsaturated, oxidized- Substance type: UVCB- Physical state: liquid- Stability under test conditions: stable- Storage condition of test material: At room temperature, protected from light
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/L- Sampling method: data not available- Sample storage conditions before analysis: data not available
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test item was tested for toxicity towards daphnia as aqueous extracts (“WAF”) from up to 100 mg test item/L (final concentration). For this purpose, different amounts of the test item were dispersed in dilution water. The elution of the water soluble part (WAF) of the test item took place on a shaking machine at 130 rpm speed over 24 h at room temperature, respectively. During that time an equilibration between the test item and the water was considered to be achieved. Thereafter, the suspensions were filtered through glass fibre filters in order to eliminate non-dissolved parts of the test item. The eluates were used for the test without further dilution steps.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM- Common name: water flea- Strain: Daphnia magna STRAUS (clone 5)- Source: of a laboratory breed (derived from Dr. M. Bergtold, BASF, D-67117 Limburgerhof; the strain originally derived from the Institut National de Recherche Chimique Appliquee, France in 1978 and cultivated since then at BASF using medium M4 acc. Elendt) was used for the test. - Age at study initiation: not older than 24h- Method of breeding: the cultivation of the daphnias is performed in a way that the animals are transferred in new test medium (see below) at an interval of 1 to 3 days followed by feeding with Desmodesmus subspicatus- and/or Chlorella-cells.- Feeding during test: during the test the daphnia were not fedACCLIMATION: data not available
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- data not available
- Test temperature:
- between 19.1°C and 20.4°C
- pH:
- between 8.00 and 8.72
- Dissolved oxygen:
- control: 8.8 mg O2/L at T0 and 8.4 mg O2/L at T48100 mg/L: 5.0 mg O2/L at T0 and 8.2 mg O2/L at T48
- Salinity:
- not applicable (freshwater)
- Nominal and measured concentrations:
- nominal concentration: 0 and 100 mg/Lmeasured concentration: 0 and 20 mg/L at T0, 0 and 16 mg/L at T48
- Details on test conditions:
- TEST SYSTEM- Test vessel: 50 mL beakers made of glass- Type (delete if not applicable): data not available- Aeration: data not available- No. of organisms per vessel: 5- No. of vessels per concentration (replicates): 4- No. of vessels per control (replicates): 4- Biomass loading rate: data not availableTEST MEDIUM / WATER PARAMETERS: test medium according Elendt M4 according to the guideline (prepared in ultra pure water)OTHER TEST CONDITIONS- Adjustment of pH: no- Photoperiod: 16h of light and 8h of darkness- Light intensity: data not availableEFFECT PARAMETERS MEASURED: after 24h and 48h the number of immobilised daphnia at each concentration of the test item is recordedTEST CONCENTRATIONS- Spacing factor for test concentrations: not applicable- Justification for using less concentrations than requested by guideline: limit test at loading rate of 100 mg/L- Range finding study- Test concentrations: 0, 1, 10 and 100 mg/L- Results used to determine the conditions for the definitive study: no immobilisation observed in the range finding test
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: no mortality observed
- Results with reference substance (positive control):
- In order to verify the sensitivity of the test system towards toxic items the effect of potassium dichromate towards different animals of this breed was tested prior to beginning of the test. The value of the EC50 (24h) in this period of time was 1.26 mg/L potassium dichromate. This value is in accordance with the recommendations given in OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test).
- Reported statistics and error estimates:
- not applicable
Any other information on results incl. tables
Table 1: result of the main test
Loading of the testitem(mg/L) (Eluates/“WAF“) | Ratio immobilised Daphnia [%] | pH-Value
| O2-Content [mg O2/L] | Temperature [°C] | |||||
24h | 48h | t0 | 48h | t0 | 48h | t0 | 48h | ||
Control (0) | 0 [0/20] | 0 [0/20] | 8.72 | 8.14 | 8.8 | 8.4 | 19.9 | 19.1 | |
100 |
| 0 [0/20] | 0 [0/20] | 8.35 | 8.00 | 5.0 | 8.2 | 20.4 | 19.1 |
Table 2: assumed values on basis of the nominal concentrations:
t24h | t48h | |
NOEC | ≥ 100 mg/L | ≥ 100 mg/L |
LOEC | > 100 mg/L | > 100 mg/L |
EL10 | > 100 mg/L | > 100 mg/L |
EL20 | > 100 mg/L | > 100 mg/L |
EL50 | > 100 mg/L | > 100 mg/L |
The test results are based on the nominal concentrations being tested as the test item was measured to be stable at in the aqueous phase at ≥ 80 % during incubation.
Table 3: results of the analytical part
NominalConcentration of the Test Item [mg/L] | mL extracted | E2924cm-1 | mg TS calculated (absolute) |
mg TS/L net TS - Blank (mg/L) | mg TS/L net TS - Blank (mg/L) + Glass wall | Recovery on Basis of t0 |
Control, t0 | 500 | 0.0097 | 0,1 | - |
|
|
100, t0 | 500 | 0.9122 | 10.1 | 20.0 | ||
Control, t48h | 415 | 0.0223 | 0.2 | - | - | - |
100, t48h | 300 | 0.4503 | 5 | 16.0 | 16.2 | 81.0 |
TS =Test item
1)LM = solvent trichlorotrifluoroethane
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- • In the control without any test item no immobilisation of the animals was observed. • In the test solutions the oxygen concentration did not drop below a value of 60% of the saturation value (~5 mg O2/L).
- Conclusions:
- EL50 (48h) > 100 mg/LNOEC (48h) > 100 mg/L
- Executive summary:
“Triglycerides, C12-24, even, saturated and unsaturated, oxidized“ was tested for acute toxicity towards daphnia according to OECD-Test Guideline 202 (version dated 13-Apr-2004). In order to investigate the influence of the test item towards the daphnias, the swimming behaviour of the animals was recorded. Under the conditions used for the test, immobilisation of the daphnias was observed at concentrations representing the water soluble part (hereafter abbreviated “WAF”, water accommodated fraction) of the test item at concentrations up to 100 mg/L. As there was no effect towards the daphnias within the screening-test the main test was performed only as a limit test using the “WAF” from 100 mg/L of the test item as the sole test concentration. The following EL-values (effective loading rate) are assumed acordingly:
t24h
t48h
NOEC
≥ 100 mg/L
≥ 100 mg/L
LOEC
> 100 mg/L
> 100 mg/L
EL10
> 100 mg/L
> 100 mg/L
EL20
> 100 mg/L
> 100 mg/L
EL50
> 100 mg/L
> 100 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.