Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
A reaction mixture containing the following components;A. Tetralithium [2(or 3), 9 (or 10), 16 (or 17), 23 (or 24)-tetrakis (3-sulfonato-propylsulfonyl) phthalocyaninato]cupurate (II)B. Trilithium [3-(2-hydroxylpropylsulfamoyl)propylsulfonyl]tris (3-sulfonato-propylsulfonyl)phthalocyaninatocupurate (II)C.Dilithium bis [3-(2-hydroxylpropylsulfamoyl)propylsulfonyl] bis (3-sulfonato-propylsulfonyl)phthalocyaninatocupurate (II)D. Lithium tris [3-(2-hydroxylpropylsulfamoyl)propylsulfonyl] (3-sulfonato-propylsulfonyl)phthalocyaninatocupurate (II)E. Tetrakis [3-(2-hydroxpropylsulfamoyl) proppylsulfonyl]phthalocyaninatocupurate (II)In the ratio A:B:C:D:E 6.25 : 25 : 37.5 : 25 : 6.25
EC number: 472-040-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Some information in this page has been claimed confidential.
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- The range-finding test was conducted between 18 November 2002 and 21 November 2002 and the definitive test between 3 February 2003 and 6 February 2003. The final report was issued 28th March 2003.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Meets the criteria for classification as reliable without restriction according to Klimisch et al (1997)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: Method C3 of Commission Directive 92/69/EEC
- Deviations:
- yes
- Remarks:
- Further refined for coloured test substances, to differentiate between a reduced growth of algae due to a true toxic effect of the chemical or due to an indirect effect, a reduction in growth by light absorption of the coloured test substance.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- Refined for coloured test substances, to differentiate between a reduced growth of algae due to a true toxic effect of the chemical or due to an indirect effect, a reduction in growth by light absorption of the coloured test substance.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Water samples were taken from the control and each test group from Experiment A alone at 0 and 72 hours for quantitative analysis. Duplicate samples were taken at each occasion and stored frozen (approximately -20°C) for further analysis if necessary.The concentration and stability of the test material in the test preparations (Experiment A only) were verified by chemical analysis at 0 and 72 hours. Analysis of the test preparations from Experiment B was not performed as this was not a requirement of the test guidelines.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test material was dissolved directly in culture medium.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- The test was carried out using Scenedesmus subspicatus strain CCAP 276120. Liquid cultures of Scenedesmus subspicatus were obtained from the Culture Collection of Algae and Protozoa (CCAP), Institute of Freshwater Ecology, The Feny House, Far Sawrey, Ambleside, Cumbria.Cultures were maintained in the laboratory by the periodic replenishment of culture medium. The culture was maintained in the laboratory at a temperature of 21 + 1°C under continuous illumination (intensity approximately 7000 lux) and constant aeration.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 24+/- 1ºC
- pH:
- 7.3-7.8
- Nominal and measured concentrations:
- Analysis of the test preparations at 0 and 72 hours showed measured test concentrations ranging from 90% to 108% of nominal and so it was considered justifiable to estimate the EC50 values in terms of nominal test concentrations only.
- Details on test conditions:
- 250 ml glass conical flasks were used.For Experiment A three flasks each containing 100 ml of algal suspension plus the relevant test material concentration were prepared for the control and each treatment group. Glass petri dishes containing 80 ml of culture medium were placed above each of the conical flasks. For Experiment B one flask containing 100 ml of algal suspension was prepared for the control and each treatment group. Glass petri dishes containing 80 ml of each test preparation were placed above each of the conical flasks.The depth of preparation (15 mm) in the petri dishes for both Experiment A and B was half the depth of the preparation in the conical flasks (30 mm). The control groups for both Experiment A and B were maintained under identical conditions but not exposed to the test material.Pre-culture conditions gave an algal suspension in log phase growth characterised by a cell density of 2.14^6 cells per ml. This suspension was diluted to a cell density of 1.96^4 cells per ml prior to use. At initiation of the study the culture contained a nominal cell density of 10^4 cells per ml.The flasks were incubated at 24 plus or minus 1°C under continuous illumination (intensity approximately 7000 lux) and constantly stirred at approximately 100 rpm for 72 hours. Samples were taken at 0 and 24 hours and the cell densities determined using a coulter Multisizer II Particle Counter. The Coulter counts performed at 24 hours showed that interference was occurring in the small particle diameter range in both control and test cultures. Microscopic examination of the cultures showed that there was a large number of extremely small algal cells present, and it was considered that it was these small cells that were being detected. It was therefore considered appropriate to monitor algal growth using a haemocytometer and light microscope for the remainder of the study period (24, 48 and 72 hours) in order to obtain an accurate count of the number of algal cells present.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 39 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Reported statistics and error estimates:
- One way analysis of variance incorporating Bartlett's test for homogeneity of variance and Dunnett's multiple comparison procedure for comparing several treatments with a control was carried out on the area under the growth curve data for Experiment A at 72 hours for the control and all test concentrations to determine any statistically significant differences between the test and control groups.
Any other information on results incl. tables
Exposure of Scenedesmus subpicatus to the test material in Experiment A gave a EbC50 (72h) value of 9.4 mg/l and a ErC50 (0 -72h) value of 39 mg/l. The No Observed Effect Concentration at 72hrs was 1.0 mg/l. Given that significant differences (greater than 10%) in the inhibition values between Experiments A and B were observed, it was considered that the effect of the test material on algal growth was not only due to a reduction in light intensity, but also due to the intrinsic toxic properties of the test material.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Given that significant differences (greater than 10%) in the inhibition values between Experiments A and B were observed, it was considered that the effect of the test material on algal growth was not only due to a reduction in light intensity, but also due to the toxicity of the test material.
- Executive summary:
Introduction
A study was performed to assess the effect of the test material on the growth of the green alga Scenedesmus subspicatus. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No 201, "Alga, Growth Inhibition Test" referenced as Method C.3 of Commission Directive 92/69/EEC (which constitutes Annex V of Council Directive 67/548/EEC) and further refined for coloured test substances, to differentiate between a reduced growth of algae due to a true toxic effect of the chemical or due to an indirect effect, a reduction in growth by light absorption of the coloured test substance (Memmert et al 1994).
Methods
Following a preliminary range-finding test, Scenedesmus subspicatus was exposed to an aqueous solution of the test material for 72 hours, under constant illumination and stirred continuously via magnetic stirrer at a temperature of 24 ± 1 °C. The test was conducted using two experimental methods performed in parallel.
Experiment A
In Experiment A the algae were exposed to test material concentrations of 3.2, 10, 32, 100 and 320 mg active ingredient ai/l. Glass petri dishes above the test vessels contained culture medium alone. Therefore, inhibition of algal growth in these test vessels was due to a combination of both the toxic effects of the test material and reduction in light intensity.
Experiment B
In Experiment B the glass petri dishes above the test vessels contained test material solutions at concentrations of 3.2, 10, 32, 100 and 320 mg ai/l. The test vessels contained algal cells in culture medium alone. Therefore inhibition of algal growth was due to a reduction in light intensity alone.
Results
Exposure of Scenedesmus subspicatus to the test material in Experiment A gave an EbC50 (72 h) value of 9.4 mg ai/l and an ErC50 (0-72 h) value of 39 mg ai/l. The No Observed Effect Concentration at 72 hours was 1.0 mg ai/l.
In Experiment B, reduction in light intensity resulted in reduction of algal growth. The EC50 values were calculated based on the concentration of the test material in the glass petri dishes above the test cultures. The reduction in growth of Scenedesmus subspicatus exposed to light passed through the test material solutions gave an EC50 (72 h) value of 14 mg ai/l and an ErC50 (0 -72 h) value of 46 mg ai/l. The No Observed Effect Concentration was 1.0 mg ai/l.
Conclusion
Given that significant differences (greater than 10%) in the inhibition values between Experiments A and B were observed, it was considered that the effect of the test material on algal growth was not only due to a reduction in light intensity, but also due to the intrinsic toxic properties of the test material.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Vi har mycket webbmaterial på ditt språk, men en del av den här sidan finns bara på engelska. Mer om vår flerspråkighetspolicy finns.
Välkommen till Echas webbplats. Alla funktioner på den här webbplatsen fungerar inte med Internet Explorer 7 (eller tidigare versioner). Det är därför bäst att du uppgraderar till en nyare version.
På den här webbplatsen används kakor. Syftet är att optimera din upplevelse av den.
Läs mer om hur vi använder kakor.