A reaction mixture containing the following components;A. Tetralithium [2(or 3), 9 (or 10), 16 (or 17), 23 (or 24)-tetrakis (3-sulfonato-propylsulfonyl) phthalocyaninato]cupurate (II)B. Trilithium [3-(2-hydroxylpropylsulfamoyl)propylsulfonyl]tris (3-sulfonato-propylsulfonyl)phthalocyaninatocupurate (II)C.Dilithium bis [3-(2-hydroxylpropylsulfamoyl)propylsulfonyl] bis (3-sulfonato-propylsulfonyl)phthalocyaninatocupurate (II)D. Lithium tris [3-(2-hydroxylpropylsulfamoyl)propylsulfonyl] (3-sulfonato-propylsulfonyl)phthalocyaninatocupurate (II)E. Tetrakis [3-(2-hydroxpropylsulfamoyl) proppylsulfonyl]phthalocyaninatocupurate (II)In the ratio A:B:C:D:E 6.25 : 25 : 37.5 : 25 : 6.25

Short-term toxicity to aquatic invertebrates

Some information in this page has been claimed confidential.

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

The range-finding test was conducted between 7 January 2003 and 9 January 2003 and the definitive test between 3 February 2003 and 5 February 2003. The final report was issued 28th March 2003.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Meets the criteria for classification as Reliable without restriction according to Klimisch et al (1997)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: 92/69/EEC,method C2 of commission directive 92/69/EEC which constuitutes Annex V of council directive 67/548/EEC

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Test material form:

solid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: None used

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Adult Daphnia were maintained in polypropylene vessels containing approximately 2 litres of reconstituted water in a temperature controlled room at 21°C. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods. Each culture was fed daily with a suspension of algae (Chlorella sp.). Culture conditions ensured that reproduction was by parthenogenesis. Gravid adults were isolated the day before initiation of the test, such that the young daphnids produced overnight were less than 24 hours old. These young were removed from the cultures and used for testing. The diet and diluents water are considered not to contain any contaminant that would affect the integrity or outcome of the study.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

250 mg CaCO3/L

Test temperature:

20.7 - 20.9 degrees C

pH:

7.8 - 8.0

Dissolved oxygen:

91 - 93 %ASV (air saturation value)

Nominal and measured concentrations:

The test substance concentration was measured spectrophotometrically using an external standard.

Details on test conditions:

TEST SYSTEM- Test vessel: 250ml glass jars- No. of organisms per vessel: 10- No. of vessels per concentration (replicates):2- No. of vessels per control (replicates):2OTHER TEST CONDITIONS- Photoperiod: 16hr light 8hr dark with 20 min dawn/dusk transitionEFFECT PARAMETERS MEASUREDAny immobilisation or adverse reactionsTEST CONCENTRATIONS- Range finding study: 0.010, 0.10, 1.0 and 10 mg/L- Test concentrations:1.8, 3.2, 5.6, 10, 18, 32, 56, 100, 180 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 180 mg/L

Conc. based on:

test mat.

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 180 mg/L

Conc. based on:

test mat.

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Conc. based on:

test mat.

Details on results:

In the range finding test, 30% immobilisation was observed at the 100 mg/L test concentration after 48h exposure. Based on this result a test concentration of 1.8 to 180 mg/L was assigned to the definitive test in an attempt to calculate a 48h EC50. However, only 35% immobilisation was observed by 48h at 180 mg/L in the definitive test so an EC50 could not be calculated. It was considered unnecessary to test at concentrations above 180 mg/L in another test as the maximum recommended test concentration in the test guideline (OECD 202 & Method C2 of Commision Directive 92/69/EEC) is 100 mg/L at which 25% immobilisation was observed in the definitive test. Due to the flat response observed in the definitive test immobilisation was observed down to concentrations of 18 mg/L and hence the NOEC after 48h exposure is 10 mg/L.

Results with reference substance (positive control):

A positive control conducted approximately every six months used potassium dichromate as the reference material. Daphnia magna was exposed to an aqueous solution of the reference material at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/l for 48 hours at a temperature of approximately 20°C under static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 3, 24 and 48 hours.

Any other information on results incl. tables

Cumulative Daphnia Immobilisation Data in the Definitive test

At 24h:

Control 0%

1.8 mg/L 0%

3.2 mg/L 0%

5.6 mg/L 0%

10 mg/L 0%

18 mg/L 0%

32 mg/L 0%

56 mg/L 15% (3/20)

100 mg/L 15% (3/20)

180 mg/L 20% (4/20)

At 48h:

Control 0%

1.8 mg/L 0%

3.2 mg/L 0%

5.6 mg/L 0%

10 mg/L 0%

18 mg/L 5% (1/20)

32 mg/L 5% (1/20)

56 mg/L 20% (1/20)

100 mg/L 25% (5/20)

180 mg/L 35% (7/20)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test substance to the freshwater invertebrate Daphnia magna has been investigated and gave a 48h EC50 value of greater than 180 mg/L. The No Observed Effect Concentration at 48h was 10 mg/L.

Executive summary:

Introduction.

A study was performed to assess the acute toxicity of the test material to Daphnia magna. The method followed that described in the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp, Acute Immobilisation Test" referenced as Method C.2 of Commission Directive 92/69/EEC (which constitutes Annex V of Council Directive 67/548/EEC).

Methods.

Following a preliminary range-finding test, twenty daphnids (2 replicates of 10 animals) were exposed to an aqueous solution of the test material at concentrations of 1.8, 3.2, 5.6, 10, 18, 32, 56, 100 and 180 mg ai*/l for 48 hours at a temperature of 21.1°C to 21.7°C under static test conditions. The number of immobilised Daphnia were recorded after 24 and 48 hours.

Results and Conclusion

The 48 -hour EC50 for the test material to Daphnia magna based on nominal test concentration was greater than 180 mg/l. The no observed efect concentration was 10 mg/l.