N-(2-methoxy-5-methylphenyl)acetamide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-(2-methoxy-5-methylphenyl)acetamide

EC Number:

230-157-2

EC Name:

N-(2-methoxy-5-methylphenyl)acetamide

Cas Number:

6962-44-3

Molecular formula:

C10H13NO2

IUPAC Name:

N-(2-methoxy-5-methylphenyl)acetamide

Details on test material:

- Name of test material (as cited in study report): Acetkresidin 34 TTRS
- Analytical purity: 98.9 %
- Expiration date of the lot/batch: 03/1998
- Stability under test conditions: yes
- Storage condition of test material: 20°C, dark

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

The test substnce was homogenized using an Ultra-Turrax and an Ultrasonic bath. Test concentrations were present as homogeneous, clear solutions without perticulates.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Strain: Brachydanio rerio (Hamilton-Buchanan)
- Source: Central Toxicology Hoechst AG
- Age at study initiation : about 7 months
- Length at study initiation 3.1 - 3.5 cm; average: 3.3 cm; SD: 0.15
- Feeding during test: twice a day ad libitum with Tetra Min

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions :same as test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

no

Test conditions

Hardness:

2.3 mmol Ca(2+) + Mg(2+) / L

Test temperature:

21.8 - 22.3 °C

pH:

7.8 - 8.3

Dissolved oxygen:

6.9 - 8.9 mg/L

Nominal and measured concentrations:

nominal: 100 mg/L
measured: 85.9 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Open glass vessel (30 cm x 22 cm x 24 cm), calibrated to 10 liters
- No. of organisms per vessel: 7
- No. of vessels per concentration : 1
- No. of vessels per control: 1


OTHER TEST CONDITIONS
12 hours illumination
no aeration

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Since the LD50 after 96 hours exceeds 100 mg/L there is no need for classification of this substance concerning the acute toxicity to fish.

Executive summary:

When testing the lethal effects of Acetkresidin 34 TTRS in zebra fish according to OECD 203 over a total of 96 hours no lethality occured at 100 mg/L. The LC50 after 96 hours exceeds 100 mg/L.