Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 211-067-2 | CAS number: 629-03-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From January to April 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- Version / remarks:
- Directive CEE 84/449/CEE annex V
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- DIBROMO- 1,6-HEXANE
- IUPAC Name:
- DIBROMO- 1,6-HEXANE
- Details on test material:
- - Name of test material (as cited in study report): DIBROMO- 1,6-HEXANE
- Substance type: colorless liquid
- Physical state: Liquid
- Analytical purity: 98,4%
- Purity test date: 98,4%
- Lot/batch No.: TVV 01-07
- Storage condition of test material: Packaged in a glass bottle and stored in aluminium foils
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Albino - ICO: OFA-SD (IOPS Caw) provided by Iffa Crédo (69210 L'Arbresle, France).
- Age at study initiation: +/- 5 weeks
- Weight at study initiation:
* Males: 161 +/- 13 g
* Females: 140 +/- 9 g
- Fasting period before study: The day before the treatment, the animals deprived from food but had access to water ad libitum 18 hours before administration. They are feeding 4 hours after treatment.
- Housing: Animals are housed for 4-7 animals of the same sex during the period of acclimatization and 5 of the same sex during the study.
The animals wer held in polycarbonate cages (dimensions 48 x 27 x 20 cm) equipped with a stainless steel lid forming a feeding trough, and a polycarbonate bottle (500 ml). Animals were lying on a litter of sawdust and dust sifted (Societe Parisienne of Sawdust 93500 Pantin, France). The analysis of residues and potential contaminant is performed regularly (Laboratoire Municipal, 76000 Rouen, France).
- Diet (e.g. ad libitum): Ad libitum - called "Rat and Mouse Maintenance" reference A04 C (U.A.R. 91360 Villemoisson-sur-Orge, France) provided throughout the duration of the study with the exception of a period of fasting during the treatment.
- Water (e.g. ad libitum): Ad libitum - drinking water filtrated on a 0.22 micron membrane (Millipore, 78140 Vélizy, France).
- Acclimation period: for a minimum period of 5 days where rats are daily observed.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3°C
- Humidity (%): 50 +/- 20%
- Air changes (per hr): air-conditioned room/ the air is not recycled but filtred.
- Photoperiod (hrs dark / hrs light): 12 H light - 12 H dark
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- polyethylene glycol
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle and amount of vehicle:
* First essay: 2000 mg/kg - Volume : 1.25 ml/kg of undiluted test item - Density of the testing substance: 1.6
* Second essay: 200 mg/kg - Volume : 10 ml/kg as a solution in PEG 300
- Justification for choice of vehicle: No justification given in the report - Doses:
- * First essai: 2000 mg/kg
* Second essai: 200 mg/kg - No. of animals per sex per dose:
- * First essay: 5 males - 5 females
* Second essay: 5 males - 5 females - Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
* clinical signs: frequently after the adminstration (hours), then at least once per day
* Mortality: frequently after the adminstration (hours), then 2 times per day
* Weight: weighted before the administration, then at day 5, 8 and 15.
- Necropsy of survivors performed: yes
- Other examinations performed:
Pathology: organs observation
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: 60% mortality : 2 males and 4 females at the end of expsoure
- Mortality:
- No mortality was noted during the observation period at a dose of 200 mg/kg and was 60% between day 2 and day 5 at a dose of 2000 mg/kg.
- Clinical signs:
- other: Clinical signs in the hours following administration of the test substance are: - At dose of 200 mg/kg: hypokinesia, piloerection and sedation up to 4 hours p.a. in all animals - At dose of 2000 mg/kg: sedation, hypokinesia, piloerection to Day 4, ptosi
- Gross pathology:
- No anomaly was observed after gross examination of the principal organs of the animals found dead during the study or sacrificed at the end of study.
Due to the absence of lesions in the macroscopic examination, no histological examination was performed.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Remarks:
- Migrated information
- Conclusions:
- Under our experimental conditions, the LD50 of the test substance DIBROMO- 1,6-HEXANE, given to the Rat by oral route was about 2000 mg/kg, with a mortality rate of 60% at this dose level .
- Executive summary:
Materials and Methods
In a first assay, the test substance was administered by oral route to a group of 10 fasted Sprague-Dawley rats (5 males and 5 females) . The test item was administered undiluted at a dose level of 2000 mg/kg taking i nto consideration the density of the test substance (d =1.6). In a second assay the test substance was administered to a group of 10 rats at a dose level of
200 mg/kg in solution in Polyethylene Glycol 300 and at a volume of 10 ml/kg. The mortality, general behaviour and bodyweight gain of the animals were observed for a period of 14 days after the single administration of the test substance. A necropsy was performed on each animal found dead during the study or sacrificed at the end of the study.Results
There was no mortality at the dose level of 200 mg/kg and the mortalfty rate was 60% after 5 days at the dose level of 2000 mg/kg. The clinical signs observed consisted in a reduction of the spontaneous activity and piloerection in the 4 hours following the administration of the test substance at 200 mg/kg or during 4 days at 2000 mg/kg. The bodyweight gain of the animals was not influenced by the treatment at 200 mg/kg and was reduced until D 5 at 2000 mg/kg and remained normal thereafter. The macroscopic examination of all the animals found dead during the study or sacrificed at the end of the study revealed no abnormality .
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.