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EC number: 700-584-3 | CAS number: 1217271-02-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2014-06-04 to 2014-06-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Version / remarks:
- 1996
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: For determination of the test item concentration five replicate samples were taken from the test concentration and three from the control at the start and at the end of the test.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Mechanical dispersion. An amount of 0.3 g test item was dissolved in 3000 mL dilution water (ISO medium) using approximately 1 hour shaking in order to obtain the concentration of 100 mg/L. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Strain: Brachydanio rerio
- Source: Black Molly Bt. 8200 Veszprém, Jutasi út 29
- Age at study initiation: juveniles
- Length at study initiation: 2 ± 1 cm
- Weight at study initiation: measured weight of 10 fish: control: 1.913 g, 100 mg/L: 2.004 g
- Feeding during study: none
ACCLIMATION
- Acclimation period: at least 12 days
- Acclimation conditions: same as test
- Type and amount of food: Fish were fed with appropriate, commercial diet for fish at least three times per week until one day before the test start.
- Health during acclimation: No mortality observed. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
- Hardness:
- 249 mg/L (as CaCO3)
- Test temperature:
- 22.2 – 22.8 ° C
- pH:
- 7.11 – 8.41
- Dissolved oxygen:
- 75.6 – 95.4 %
- Salinity:
- not applicable
- Nominal and measured concentrations:
- nominal: 100 mg/L, the measured concentrations varied between 99 and 104 % of the nominal concentration.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquariums with 3 Litre test liquid.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: did not exceed 1 g fish/L
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
EFFECT PARAMETERS MEASURED:
The fish were observed at approximately 3, 6, 24, 48, 72 and 96 hours after start of the test for signs of intoxication and mortality.
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: Toxic effects were not observed during the preliminary test therefore a limit test was carried out with one treatment group at a concentration of 100 mg/L (limit concentration) and a concurrent control.
- Test concentrations in range finding study: 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: no toxic effects observed - Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: No sub-lethal effects were observed.
- Mortality of control: No mortality occurred in the control.
- Other adverse effects control: No adverse effects observed. - Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results of this study, the test item SIKA Hardener MI (SIKA Härter MI) had no toxic effect at the limit concentration of 100 mg/L on Zebrafish. Accordingly, the 96-h LC50 value was determined to be > 100 mg/L. The 96-h NOEC was determined to be 100 mg/L.
- Executive summary:
In an acute Toxicity Test of SIKA Hardener MI (SIKA Härter MI) on Zebrafish (Brachydanio rerio) conducted according to OECD guideline 203 (1992), EU method C. 1 (2008) and OPPTS 850.1075 (1996), the acute toxicity of the test item on fish was evaluated. A limit test was performed in which the test animals were exposed to aqueous test media containing the test item for 96 hours at one concentration (100 mg/L) plus a control. Based on the results of an analytical method validation the concentration of the test item was expected to remain within 80 - 120 % of the nominal over the testing period, therefore, the experiment was carried out under static conditions. The measured test item concentration remained within ± 20 % of the nominal concentrations over the test period of 96 hours; therefore, the biological results are based on the nominal concentration. Mortality and any sub-lethal effects were not observed during the 96-h exposure period neither in the treated nor in the control group. Accordingly, the 96-h NOEC was determined to be 100 mg/L and the LOEC and the LC50 values were determined to be higher than 100 mg/L.
Reference
Description of key information
In a 96-hour acute toxicity study on fish the LC50 value was determined to be greater than 100 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 100 mg/L
Additional information
In an acute Toxicity Test of Sika Hardener MI (Sika Härter MI) on Zebrafish (Brachydanio rerio) conducted according to OECD guideline 203 (1992), EU method C. 1 (2008) and OPPTS 850.1075 (1996), the acute toxicity of the test item on fish was evaluated. A limit test was performed in which the test animals were exposed to aqueous test media containing the test item for 96 hours at one concentration (100 mg/L) plus a control. Based on the results of an analytical method validation the concentration of the test item was expected to remain within 80 - 120 % of the nominal over the testing period, therefore, the experiment was carried out under static conditions. The measured test item concentration remained within ± 20 % of the nominal concentrations over the test period of 96 hours; therefore, the biological results are based on the nominal concentration. Mortality and any sub-lethal effects were not observed during the 96-h exposure period neither in the treated nor in the control group. Accordingly, the 96-h NOEC was determined to be 100 mg/L and the LOEC and the LC50 values were determined to be higher than 100 mg/L.
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