2-Propenamide, N-(2-hydroxyethyl)-

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21 June 2006 to 11 September 2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.3110 (Ready Biodegradability)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Lot number: 050804
Purity: >99.0%

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): The aeration tank at Worlingworth sewage treatment works, which treats predominantly domestic waste.
- Laboratory culture: Aliquots (25 ml) of a homegenised sample were filtered through dried and pre-weighed Whatman GF/C filter papers.
- Concentration of sludge: 30 mg/L
- Water filtered: yes
- Type and size of filter used, if any:Whatman GF/C

Duration of test (contact time):

28 d

Initial conc.:

38.4 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST SYSTEM
- Culturing apparatus: 2 L Erlenmeyer flasks
- Number of culture flasks/concentration: 3

SAMPLING
- Sampling frequency: samples (50 mL) were removed on days 0, 3, 5, 7, 10, 14, 21, 28 of the test

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

% degradation (DOC removal)

Value:

98.4

Sampling time:

28 d

Details on results:

DOC removal by the mixtures containing HEAA was rapid and equivalent to 10% of the mean initial value by approximately day 5, 70% by day 8 and 97.7% after 10 days. A biodegradation plateau was considered achieved after 10 days.

Results with reference substance:

The mean level of biodegradation of sodium benzoate was 98.1% after 3 days. A biodegradation plateau was considered achieved after 3 days and the maximum level of degradation of the reference substance reached 100% on day 7.

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

DOC removal by the test substance is greater than 70% within 28 days and this level reached within ten days of degradation reaching 10%. Hence, HEAA can be considered as readily biodegradable.

Executive summary:

The ultimate biodegradability of HEAA was assessed in the DOC die-away test according to EU Method C4 and OECD Guideline 301A.

DOC removal by the test substance is greater than 70% within 28 days and this level reached within ten days of degradation reaching 10%. Hence, HEAA can be considered as readily biodegradable.

Description of key information

The ultimate biodegradability of HEAA was assessed in the DOC die-away test according to EU Method C4 and OECD Guideline 301A.

DOC removal by the test substance is greater than 70% within 28 days and this level reached within ten days of degradation reaching 10%. Hence, HEAA can be considered as readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater