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EC number: 252-743-7 | CAS number: 35835-94-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Version / remarks:
- 1987
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.3 (Acute Toxicity (Dermal))
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Harlan U.K. Ltd., Blackthorn, Bicester, Oxon, U.K. and Charles River (UK) Ltd., Margate, Kent, UK
- Age at study initiation: 10-14 weeks
- Weight at study initiation: 231 - 255 g (males), females 200 - 232 g (females)
- Fasting period before study: no
- Housing: individually during exposure period, in groups of five for the remainder of the study
- Diet (e.g. ad libitum): ad libitum (Rat and Mouse Expanded Diet No. 1, Special Diets Services Limited, Wi tham, Essex, U.K.)
- Water (e.g. ad libitum): tap water, ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-24
- Humidity (%): 51-61
- Air changes (per hr): approx. 15
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: back and flanks
- % coverage: 10% of total body surface
- Type of wrap if used: A piece of surgical gauze was placed over the treatment area and semi-occluded with a piece of self-adhesive bandage (HYPERTIE). The bandage was further secured with a piece of BLENDERM wrapped around each end.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): wiped with cotton wool moistened with distilled water to remove any residual test material
- Time after start of exposure: 24 h
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2000 mg/kg bw
- For solids, paste formed: moistened with distilled water
- Duration of exposure:
- 24 h
- Doses:
- males: 2000 mg/kg bw
females: 1414, 2000 and 2828 mg/kg bw - No. of animals per sex per dose:
- 5
- Control animals:
- not required
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: 0.5, 1, 2 and 4 hours after dosing and subsequently once daily for 14 days
- Frequency of weighing: day 0, 7, 17 or at death
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs - Statistics:
- LD50 and 95% confidence limits of the test material for females only were calculated using the method of Litchfield and Wilcoxon (J. Pharmacol 96, 1949)
Results and discussion
Effect levels
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 1 437 mg/kg bw
- Based on:
- act. ingr.
- 95% CL:
- >= 618 - <= 3 338
- Mortality:
- 3/5 females died at 1414 mg/kg bw, 1/5 males and 2/5 females died at 2000 mg/kg bw, 4/5 females died at 2828 mg/kg bw
Deaths were noted 1-2 days after dosing. - Clinical signs:
- other: Incidents of systemic toxicity, mostly confined to decedents, included hunched posture, lethargy, ptosis and decreased respiratory rate. Isolated incidents of ataxia with or without splayed gait were a1 so noted in one male and one female treated with 200
- Gross pathology:
- Common abnormalities noted at necropsy induced abnormally red or haemorrhagic lungs, dark livers or patchy pallor of the liver, dark or pale kidneys and haemorhage of the small intestines. Isolated incidents of haemorrhage of the gastric mucosa or haemorrhage of the site of application were also noted in the 2000 mg/kg dose group. No abnormalities were noted at necropsy of surviving animals at the end of the study.
- Other findings:
- Very slight to well-defined erythema was elicited by the test material. Other adverse dermal reactions noted were necrosis, small superficial scattered scabs, hardened light brown-coloured scabs and glossy skin. These dermal reactions sometimes precluded accurate assessment of the erythema and oedema.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The dermal LD50 and 95% confidence limits of the test material were calculated to be 1437 (618-3338) mg/kg bw in females. There was no significant difference in toxicity to males or females.
- Executive summary:
This study was performed to assess the acute dermal toxicity of Methyltriphenylphosphonium chloride in the Sprague-Dawley strain rat. The method followed that described in OECD Guideline 402 (1987) and EU method B3 (1992).
A group of ten animals (five males and five females) was given a single 24- hour semi -occluded dermal application to intact skin at dose level of 2000 mg/kg bw. A further two groups of females were similarly treated at dose levels of 1414 and 2828 mg/kg bw. The surviving animals were observed for fourteen days after the day of dosing. All animals were subjected to gross pathological examination.
The majority of deaths were noted 1-2 days after dosing. Two females treated with 2828 mg/kg were found dead at the 4-hour observation. Incidents of systemic toxicity, mostly confined to decedents, included hunched posture, lethargy, ptosis and decreased respiratory rate. Isolated incidents of ataxia with or without splayed gait were also noted in one male and one female treated with 2000 mg/kg bw. All other surviving animals treated with 2000 mg/kg bw appeared normal throughout the study. Evidence of irritation of the treated skin sites was also noted.
All surviving animals showed bodyweight gain during the study.
Common abnormalities noted at necropsy included abnormally red or hemorrhagic lungs, dark livers or patchy pallor of the liver, dark or pale kidneys and hemorrhage of the small intestines. Isolated incidents of hemorrhage of the gastric mucosa or hemorrhage of the site of application were also noted in the 2000 mg/kg bw dose group. No abnormalities were noted at necropsy of surviving animals at the end of the study.
The dermal LD50 and 95% confidence limits of the test material were calculated by the method of Litchfield and Wilcoxon to be 1437 (618-3338) mg/kg bw in females. There was no significant difference in toxicity to males or females.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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