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EC number: 241-253-9 | CAS number: 17211-15-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the analogue justification attached to IUCLID section 13
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 28.13 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.56 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.24 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.65 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.32 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item caused significant inhibition of algal growth at the following concentrations:
3.2 – 100 mg/L (growth rate)
1 – 100 mg/L (yield)
NOEC for the growth rate was 1.0 mg/L and EC50 (72h) was > 28.13 mg/L based on nominal concentrations. - Executive summary:
One valid experiment was performed.
The study was performed according to OECD 201 (GLP) using 6 concentrations ranging from 0.32 to 100 mg/L. Incubation time (test system Desmodesmus subspicatus) was 72 hours.
Significant inhibition of algal growth was observed at the following concentrations:
3.2 – 100 mg/L (growth rate)
1 – 100 mg/L (yield)
At the start and at the end of the test, the content of the test item in the test solutions was determined using a photometric method. In the two lowest treatments, the values determined were below the LOQ (1 mg/L Phosphate resp.1.50 mg/L test item). The measurable concentrations lay between 95 % and 102 % of the nominal concentrations at the beginning of the test and between 59 % and 106 % of the nominal concentrations at the end of the test. These values deviate by more than ± 20% from the nominal values. Since the low values are below the LOQ, the geometric mean of the highest concentration is formed. Since the lower concentrations were prepared by dilution of the stock solution, the real concentrations were calculated on the basis of the geometric mean of the
measured concentration in the highest concentrated treatment and the respective Dilution factor. These calculated values correspond to the nominal values.
The following results for the test item were determined:
Endpoint
NOEC
LOEC
EC10
EC50
Growth Rate
1 mg/L
3.2 mg/L
0.56 mg/L
28.13 mg/L
Yield
0.32 mg/L
1 mg/L
0.65 mg/L
2.24 mg/L
Note: According to the guideline, NOEC is determined by comparing of the respective treatment with the blank control. Statistically insignificant variation is considered as “no observed effect”, although the EC10 which is read from the graph toxicity vs. concentration may lie lower.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06. Aug. 2018 to 09. Aug. 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- OECD Guideline for the Testing of Chemicals No. 201, adopted 23. Mar. 2006, Annex
5 corrected: 28 July 2011 “Freshwater Alga and Cyanobacteria, Growth Inhibition
Test” - Deviations:
- yes
- Remarks:
- See section "Any other information on material and methods incl. tables" - Stock solution autoclaved for 20 min instead of 15 min. - Temperature in the Experiment - Temperature during pre-culture
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- 2009
- Deviations:
- yes
- Remarks:
- See section "Any other information on material and methods incl. tables"
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- CAS No.: 14306-25-3
- EC No.: 238-242-6
- Source and lot/batch No.of test material: NA2253
- Expiration date of the lot/batch: June 2019
- Purity test date: 16 May 2017
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test item was stored in a closed vessel at room temperature (20 ± 5°C) protected from light and humidity. - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.32 / 1 / 3.2 / 10 / 32 / 100 mg/L nominal concentration.The concentrations to be tested are based on non GLP pre-tests.
- Sampling method: At test start (0 h) and test end (72 h), all samples were analysed. All samples will be measured with and without disgestion. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution containing 100.2 mg/L in algal medium (demineralised water enriched with minerals but without algae) was prepared. The lower treatments were prepared by dilution
of this stock solution with algal medium.
- Controls: yes, algal medium and algae - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Unicellular freshwater green alga
- Strain: 86.81 SAG
- Source: The culture of Desmodesmus subspicatus was obtained in January 2016 by MBM S
ciencebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen).
- Method of cultivation: The algae are kept as stock culture on solid agar at 2 - 8 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- 72 ± 3 hours
- Hardness:
- n.a.
- Test temperature:
- 16.0 - 28.2°C
- pH:
- for Information see section "Any other information on materials and methods incl. tables".
In the test solutions and the blank control solution, the pH-value is measured at the start and the end of the test. The pH of the blank control should not vary by more than 1.5 Units. - Dissolved oxygen:
- n.a.
- Salinity:
- n.a.
- Conductivity:
- for Information see section "Any other information on materials and methods incl. tables".
- Nominal and measured concentrations:
- - control / 0.32 / 1 / 3.2 / 10 / 32 / 100 mg/L test item (nominal);
- control / 0.3 / 1 / 3.2 / 10 / 32 / 100 mg/L (calculated on the basis of the geometric mean of the
measured concentration in the highest concentrated treatment and the respective dilution
factor)
for more information see section: "Any other information on materials and methods incl. tables". - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks (total volume 65 ml)
- Type (delete if not applicable): open (covered with perforated plastic foil acting as a stopper)
- Material, size, headspace, fill volume: 65 ml glass flasks filled with 45 ± 1 mL of the respective test solution; shaken on an orbital shaker to keep the algae in suspension.
- Initial cells density: 2.3 * 103 cells/mL
- Control end cells density: 380170 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised water was used to perform the OECD media
according to OECD 201
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Photoperiod: continuous lighting
- Light intensity and quality: 5000 lux (range: 4440 to 8880 Lux)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: The real cell concentration at the beginning of the test was
measured with an electronic particle counter in the blank control solution. This measured value was used as start cell concentration for all treatments. Every 24 ± 1 hours, the cell number in all vessels was measured with an electronic particle counter.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Range finding study: non-GLP range-finder was performed without any additional information
- Test concentrations: 0.32 / 1 / 3.2 / 10 / 32 / 100 mg/L (nominal) - Reference substance (positive control):
- yes
- Remarks:
- yes potassium dichromate (K2Cr2O7, CAS No. 7778-50-9)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 28.13 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.56 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.24 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.65 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.32 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): The microscopic examination after 72 h of test duration did reveal a normal and healthy appearance of the algae in the test item concentrations 0.32, 1, 3.2, 10 mg/L and in the control. In the highest test item concentrations 32 and 100 mg/L no cells were visible after 72 h.
- Any stimulation of growth found in any treatment: slight groth inhibition at the lowest test item concentration 0.32 mg/L: 0.7% (growth rate) and 3.3% (yield) stimulation compared to blank control.
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a separate
reference test.
72h EC50: 0.79 mg/L (growth rate)
72h EC50: 0.39 mg/L (yield)
The values lay within the range of the laboratory (growth rate 0.73 - 1.10 mg/L, yield 0.21 – 0.66 mg/L). - Reported statistics and error estimates:
- EC values were calculated by a 3-parametric concentration distribution function. The NOEC andLOEC were determined by Dunnett’s Multiple t-test procedure. Prior to the Dunnett’s test, a Shapiro-
Wilk’s test and a Levene’s test were conducted to test for normality and homogeneity of variance,
respectively, over treatments at each time point. Calculation of results was performed with the help of validated software (Microsoft Excel®). The estimation of the biological data was accomplished using the software ToxRat® Professional, version 3.2.1, ToxRat Solutions GmbH. - Validity criteria fulfilled:
- yes
- Remarks:
- For further details please see section “Any other information on results incl. tables”.
- Conclusions:
- The test item caused significant inhibition of algal growth at the following concentrations:
3.2 – 100 mg/L (growth rate)
1 – 100 mg/L (yield)
NOEC for the growth rate was 1.0 mg/L and EC50 (72h) was > 28.13 mg/L based on nominal concentrations. - Executive summary:
One valid experiment was performed.
The study was performed according to OECD 201 (GLP) using 6 concentrations ranging from 0.32 to 100 mg/L. Incubation time (test system Desmodesmus subspicatus) was 72 hours.
Significant inhibition of algal growth was observed at the following concentrations:
3.2 – 100 mg/L (growth rate)
1 – 100 mg/L (yield)
At the start and at the end of the test, the content of the test item in the test solutions was determined using a photometric method. In the two lowest treatments, the values determined were below the LOQ (1 mg/L Phosphate resp.1.50 mg/L test item). The measurable concentrations lay between 95 % and 102 % of the nominal concentrations at the beginning of the test and between 59 % and 106 % of the nominal concentrations at the end of the test. These values deviate by more than ± 20% from the nominal values. Since the low values are below the LOQ, the geometric mean of the highest concentration is formed. Since the lower concentrations were prepared by dilution of the stock solution, the real concentrations were calculated on the basis of the geometric mean of the
measured concentration in the highest concentrated treatment and the respective Dilution factor. These calculated values correspond to the nominal values.
The following results for the test item were determined:
Endpoint
NOEC
LOEC
EC10
EC50
Growth Rate
1 mg/L
3.2 mg/L
0.56 mg/L
28.13 mg/L
Yield
0.32 mg/L
1 mg/L
0.65 mg/L
2.24 mg/L
Note: According to the guideline, NOEC is determined by comparing of the respective treatment with the blank control. Statistically insignificant variation is considered as “no observed effect”, although the EC10 which is read from the graph toxicity vs. concentration may lie lower.
Referenceopen allclose all
Growth Rate, Yield
From the cell numbers, the Growth Rate μ and the Yield were calculated. The means and standard deviations at the end of the test are given in the following table:
Table Growth Rate μ, Yield
Nominal Concentration in mg/L |
Parameter |
Growth Rate (0-72h) [day-1] |
Yield (0-72h) |
Blank control |
Mean |
1.70 |
377870 |
SD |
0.04 |
44906 |
|
0.32 |
Mean |
1.71 |
390320 |
SD |
0.02 |
20116 |
|
1 |
Mean |
1.63 |
307047 |
SD |
0.02 |
18042 |
|
3.2 |
Mean |
1.35 |
131560 |
SD |
0.09 |
34758 |
|
10 |
Mean |
0.90 |
31633 |
SD |
0.04 |
4020 |
|
32 |
Mean |
0.77 |
21100 |
SD |
0.04 |
2445 |
|
100 |
Mean |
0.77 |
20733 |
SD |
0.05 |
3251 |
Inhibition
The following mean inhibition values were calculated for the Growth Rate μ and the Yield.
Table Inhibition Values
Nominal concentration in mg/L |
% Inhibition |
|
Growth Rate (0-72h) |
Yield (0-72h) |
|
Blank control |
0 |
0 |
0.32 |
-0.7 |
-3.3 |
1 |
3.9 |
18.7 |
3.2 |
20.8 |
65.2 |
10 |
47.3 |
91.6 |
32 |
54.6 |
94.4 |
100 |
55.0 |
94.5 |
Table Validity criteria for OECD 201.
Parameter |
Validity criteria |
Observed value |
Assessment |
Increase factor biomass |
factor 16 in 72 h |
165 |
Valid |
Mean coefficient of variation of daily growth rates |
max. 35% |
11% |
Valid |
Coefficient of variation of average growth rate during the whole test period |
max. 7% |
2% |
Valid |
Biological Results
The following values were determined:
Table Biological Results of the Test Item
Parameter |
Value |
95% confidence interval |
NOEC (Growth Rate) 72 h |
1 mg/L |
-- |
NOEC (Yield) 72 h |
0.32 mg/L |
-- |
LOEC (Growth Rate) 72 h |
3.2 mg/L |
-- |
LOEC (Yield) 72 h |
1 mg/L |
-- |
72h ErC10 |
0.56 mg/L |
0.19 – 1.66 mg/L |
72h EyC10 |
0.65 mg/L |
0.41 – 1.04 mg/L |
72h ErC50 |
28.13 mg/L |
7.13 – 105.55 mg/L |
72h EyC50 |
2.24 mg/L |
1.31 – 3.81 mg/L |
Description of key information
Growth rate:
NOEC (72h): 1 mg/L (D. subspicatus,OECD 201, nominal, read-across)
EC50 (72h): 28.13 mg/L (D. subspicatus,OECD 201, nominal, read-across)
Yield:
NOEC (72h): 0.32 mg/L (D. subspicatus,OECD 201, nominal, read-across)
EC50 (72h): 2.24 mg/L (D. subspicatus,OECD 201, nominal, read-across)
Key value for chemical safety assessment
Additional information
No data are available on the toxicity towards aquatic algae and cyanobacteria of "Esters of sodium phosphate with myo-Inositol, plant-derived" (CAS No. 17211-15-3).
Therefore, data from the analogue substance "Esters of sodium hydrogen phosphate with myo-Inositol, plant-derived" (CAS 14306 -25 -3; EC 238 -242 -6) were used for read across. This read-across is in accordance with Regulation (EC) No. 1907/2006 Annex XI, 1.5. In this case of read-across, the best suited (highest degree of structural similarity, nearest physico-chemical properties) read-across substance was used for the assessment. This read-across is justified in within the analogue justification in IUCLID Section 13.
One valid experiment was performed.
The study was performed according to OECD 201 (GLP) using 6 concentrations ranging from 0.32 to 100 mg/L. Incubation time (test system Desmodesmus subspicatus) was 72 hours.
Significant inhibition of algal growth was observed at the following concentrations:
3.2 – 100 mg/L (growth rate)
1 – 100 mg/L (yield)
At the start and at the end of the test, the content of the test item in the test solutions was determined using a photometric method. In the two lowest treatments, the values determined were below the LOQ (1 mg/L Phosphate resp.1.50 mg/L test item). The measurable concentrations lay between 95 % and 102 % of the nominal concentrations at the beginning of the test and between 59 % and 106 % of the nominal concentrations at the end of the test. These values deviate by more than ± 20% from the nominal values. Since the low values are below the LOQ, the geometric mean of the highest concentration is formed. Since the lower concentrations were prepared by dilution of the stock solution, the real concentrations were calculated on the basis of the geometric mean of the measured concentration in the highest concentrated treatment and the respective Dilution factor. These calculated values correspond to the nominal values.
The following results for the test item were determined:
Endpoint |
NOEC |
LOEC |
EC10 |
EC50 |
Growth Rate |
1 mg/L |
3.2 mg/L |
0.56 mg/L |
28.13 mg/L |
Yield |
0.32 mg/L |
1 mg/L |
0.65 mg/L |
2.24 mg/L |
Note: According to the guideline, NOEC is determined by comparing of the respective treatment with the blank control. Statistically insignificant variation is considered as “no observed effect”, although the EC10 which is read from the graph toxicity vs. concentration may lie lower.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.