Ethanol, 2,2'-iminobis-, N-tallow alkyl derivs., N-oxides

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates:

In the acute immobilisation test according to OECD guideline 202 the 48 hours acute median Effective Loading Rate (EL50) of Humectol LYS MOD on Daphnia magna is 0.0815 mg/L, the 95% confidence interval with lower limit of 0.0696 mg/L and upper limit of 0.0954 mg/L. No Observed Effect Loading Rate (NOELR) and Lowest Observed Effect Loading Rate (LOELR) over the 48 hours exposure period are 0.021 and 0.047 mg/L of Humectol LYS MOD respectively.

Toxicity to aquatic algae and cyanobacteria:

In a 72 hours Growth Inhibition Test on Pseudokirchneriella subcapitata according to OECD guideline 201 an ErL50 (growth rate) and EyL50 (yield) of Humectol LYS MOD of >100 mg/L was determined, respectively. The 72 hours No Observed Effect Loading Rate (NOELR) and the Lowest Observed Effect Loading Rate (LOELR) is >100 mg/L of Humectol LYS MOD, respectively.

Additional information

Short-term toxicity to aquatic invertebrates:

The test item Humectol LYS MOD was evaluated for acute toxicity on Daphnia magna according to OECD Guideline No. 202. The acute toxicity study of Humectol LYS MOD on Daphnia magna was performed to determine the 48 hours Effective concentration (EL50) and to determine Lowest Observed Effect Nominal Loading Rate (LOELR) and No Observed Effect Nominal Loading Rate (NOELR). Daphnids were exposed for the test item by aqueous exposure (treatment) under semi-static condition. Daphnids were observed at 24 and 48 hours for signs of toxicity and immobilization.
Based on the results of a dose range finding study, main study was conducted at the nominal loading rates of 0.010, 0.021, 0.047, 0.103, 0.227 and 0.500 mg/L of Humectol LYS MOD of test medium along with control. No clinical signs of toxicity were observed in control and at the nominal loading rate of 0.010 mg/L during the 48 hours observation period. Clinical signs of daphnids localized on bottom of container and lethargy was observed at the nominal loading rates of 0.021, 0.047, 0.103 and 0.227 mg/L during the 48 hours observation period. Clinical signs of daphnids localized on bottom of container and lethargy was observed at the nominal loading rates of 0.500 mg/L during the 24 hours observation period. The immobilization percentages of 0, 10, 35, 60, 80 and 100% were observed at the nominal loading rates of 0.010, 0.021, 0.047, 0.103, 0.227 and 0.500 mg/L respectively during the 48 hours observation period.
Based on the above results of the experiment and under experimental conditions employed, it is concluded that the 48 hour acute median effective concentration (EL50) of Humectol LYS MOD on Daphnia magna is 0.0815 mg/L; the 95 % confidence interval with lower limit of 0.0696 mg/L and upper limit of 0.0954 mg/L. NOELR and LOELR over the 48 hours exposure period are 0.021 and 0.047 mg/L of Humectol LYS MOD, respectively.

Toxicity to aquatic algae and cyanobacteria:

The test item Humectol LYS MOD was assessed for Growth Inhibition on Pseudokirchneriella subcapitata according to OECD Guideline 201.

A dose range finding study was conducted with six nominal loading rates of 0.01, 0.1, 1.0, 10.0, 50.0 and 100.0 mg/L along with control. Each loading rate contains 2 replicates except control possessing 3 replicates. The algal growth (cell density) was assessed at 24, 48 and 72 hours of post-exposure during the test. During the dose range finding study, exponentially growing cells (5709 cells/mL) were exposed to range of selected nominal loading rates. A growth rate inhibition of 0.36, 0.08, 0.48, 0.02, -0.23 and -0.45%, was observed at the tested nominal loading rates of 0.01, 0.1, 1.0, 10.0, 50.0 and 100.0 mg/L of Humectol LYS MOD respectively as compared with the control. Percent inhibition in yield of 1.8, 0.4, 2.4, 0.1, 2.8 and -2.2% was observed at the tested nominal loading rates of 0.01, 0.1, 1.0, 10.0, 50.0 and 100.0 mg/L of Humectol LYS MOD respectively as compared with the control.
Based on the results of dose range finding study, a limit test was conducted at the nominal loading rate of 100 mg/L along with control having 6 replicates. The algal growth (cell density) was assessed at 24, 48 and 72 hours post-exposure. Exponentially growing alga cells (5462 cells/mL) were exposed to the limit nominal loading rate. A growth rate inhibition of 0.01 % was observed at the nominal loading rate of 100 mg/L as compared with the control. Similarly, inhibition in yield of 0.1% was observed at the nominal loading rate of 100 mg/L as compared with the control.
From the experiment conducted under standard experimental conditions, the 72 hours ErL50 (growth rate) and EyL50 (yield) of Humectol LYS MOD on Pseudokirchneriella subcapitata is >100 mg/L. The 72 hours No Observed Effect Loading Rate (NOELR) and the Lowest Observed Effect Loading Rate (LOELR) is >100 mg/L of Humectol LYS MOD.