Registration Dossier
Registration Dossier
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Diss Factsheets
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EC number: 233-271-0 | CAS number: 10102-43-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
An Ames test gave positive results: NO induced gene mutations in bacteria at non cytotoxic concentrations, with and without metabolic activation. The exposure concentrations were maximised: 5000 ppm. The reliability of this GLP-compliant guideline test is Klimisch 1.
A chromosome aberration assay on CHO cells indicated a clastogenic effect at highly cytotoxic concentrations without metabolic activation. The exposure concentrations were maximised: 1800 ppm. Metabolic activation has not been tested. Due to this deviation, the reliability of this GLP-compliant test has been established as Klimisch 2.
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (positive)
Genetic toxicity in vivo
Description of key information
An in vivo rat micronucleus test was negative after inhalation up to the maximum tolerated dose (150 ppm leading to mortality). The reliability of this GLP-compliant guideline test is Klimisch 1.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Mode of Action Analysis / Human Relevance Framework
Besides these studies with forced exposure, the relevance of in vivo exposure to NO should also be discussed. First, it is a highly toxic chemical where actual exposures are well controlled due to acute toxicity and induction of methemoglobinemia. Secondly, NO is a highly reactive chemical: in actual exposure conditions it would undergo transformation into NO2 and other NOx/HNO3 species due to humidity or oxygen in air, well before reaching any target tissue. Due to this degradation, any leak becomes clearly visible as NO2 is an orange-colour gas. Last, NO it is an endogenous compound acting as an important cellular signalling molecule in many physiological and pathological processes, with a half-life of ca. 5 seconds.
Additional information
Justification for classification or non-classification
Not classified based on an in vivo genotoxicity study with maximized exposure.
Actual exposure potential is negligible due to high toxicity, high instability of this chemical, and easiness to spot any leak based on orange-color.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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