Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-570-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2018-01-09 to 2018-04-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Study performed according to the OECD 301F guideline and under GLP conditions. The validity criteria were fulfilled.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- None
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Species/Origin: Aerobic activated sludge (microorganisms from a domestic waste¬water treatment plant) was supplied by the sewage treatment plant of Rossdorf, Germany.
- Conditioning: The aerobic activated sludge used for this study was deposited for 15 min, washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in test water and centrifuged again. This procedure was done three times. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 3.5 g dry material per litre were mixed with test water and aerated overnight. This suspension was used for the experiment.
The washed activated sludge was pre-conditioned in test water for a maximum of 2 days. - Duration of test (contact time):
- 60 d
- Initial conc.:
- 3.021 other: mg oxygen per mg test item
- Based on:
- ThOD
- Remarks:
- NH4
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Surrounding Type: Climatic chamber
- Temperature: 22 °C ± 1°C
- Light Conditions: Darkness
- pH-Value: 6.5 to 6.7 (measured at the start of the test and adjusted to 7.4 with Sodium Hydroxide) 7.2 to 7.8 (measured at day 63)
- Reconstituted Water: The following stock solutions with analytical grade salts was prepared:
a) 8.5 g KH2PO4, 21.75 g K2HPO4, 33.4 g Na2HPO4 x 2 H2O, 0.5 g NH4Cl filled up with pure water to 1000 mL volume; the pH should be 7.4 ± 0.2.
b) 22.5 g MgSO4 x 7 H2O filled up with pure water to 1000 mL volume
c) 36.4 g CaCl2 x 2 H2O filled up with pure water to 1000 mL volume
d) 0.25 g FeCl3 x 6 H2O filled up with pure water to 1000 mL volume
In order to avoid precipitation of iron hydroxide during storage, one drop of concentrated HCl per litre was added to stock solution d) before storage. 10 mL of stock solution a) and 1 mL of the stock solutions b) - d) were combined and filled up to a final volume of 1000 mL with pure water.
- Solubilising agent: Silicone oil AR20 was used as an emulsifying agent. (1%)
TEST SYSTEM
- The closed test flasks were incubated in a climatised chamber under continuous stirring. The consumption of oxygen was determined by measuring the change of pressure in the flasks. Evolved carbon dioxide was absorbed in an aqueous solution (45%) of potassium hydroxide.
- Number of culture flasks/concentration: 2
The test flasks were closed gas-tight by a measuring head. An appropriate CO2-absorber (KOH as an aqueous solution) was used for trapping the produced carbon dioxide. The amount of O2 consumed by the activated sludge was calculated from the decrease of pressure in the reaction vessel.
SAMPLING
O2 consumed daily by the activated sludge was calculated from the decrease of pressure in the reaction vessel.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 (activated sludge + silicone oil + test water)
- Abiotic sterile control: 1
- Toxicity control: 1
- Other: Procedure control : (Ref. Item + activated sludge + silicone oil + test water) - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- No data.
- Test performance:
- No data.
- Parameter:
- % degradation (O2 consumption)
- Value:
- 54
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 68
- Sampling time:
- 60 d
- Details on results:
- TEST ITEM: The mean biodegradation of 10% of PATCHOULI EO FRACTION PATCHOULOL RICH was reached at day 7 (ThODNH4). At the end of the 10-day window at day 17, the mean degradation of PATCHOULI EO FRACTION PATCHOULOL RICH was 38% (ThODNH4).The mean biodegradation after 28 days was 54% (ThODNH4) and 68% (ThODNH4) after 60 days.
TOXICITY CONTROL: In the toxicity control containing both, the test item and the reference item sodium benzoate, 37% (ThODNH4) biodegradation was noted within 14 days, 53% (ThODNH4) biodegradation after 28 days and 57% (ThODNH4) after 60 days of incubation.
According to the test guideline, the test item can be assumed to be not inhibitory to the aerobic activated sludge microorganisms because degradation was >25% within 14 days. - Results with reference substance:
- The reference item sodium benzoate was sufficiently degraded to 85% after 14 days and to 89% after 28 days of incubation, thus confirming the suitability of the aerobic activated sludge inoculum used.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- With a mean biodegradation rate (ThOD-NH4) of 54% after 28 days of incubation, the test item is considered not readily biodegradable.
- Executive summary:
The test item Patchouli EO Fraction Patcoulol Rich was investigated for its ready biodegradability in a manometric respirometry test, according to the OECD 301F guideline and under GLP compliance. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
The mean biodegradation of 10% of PATCHOULI EO FRACTION PATCHOULOL RICH was reached at day 7 (ThODNH4). At the end of the 10-day window at day 17, the mean degradation of PATCHOULI EO FRACTION PATCHOULOL RICH was 38% (ThODNH4).The mean biodegradation after 28 days was 54% (ThODNH4) and 68% (ThODNH4) after 60 days.
Therefore, the test item is considered not readily biodegradable according to test guideline.
The reference item sodium benzoate was sufficiently degraded to 67% after 14 days and to 89% after 28 days of incubation, thus confirming the suitability of the aerobic activated sludge inoculum used.
In the toxicity control containing both, the test item and the reference item sodium benzoate, 37% biodegradation was noted within 14 days and 53% biodegradation after 28 days of incubation. According to the test guidelines, the test item can be assumed to be not inhibitory to the aerobic activated sludge microorganisms because degradation was >25% within 14 days.
Reference
pH-Values at the End of the Test
Flask No. |
Treatment |
pH-value |
1 |
PATCHOULI EO FRACTION PATCHOULOL RICH |
7.3 |
2 |
PATCHOULI EO FRACTION PATCHOULOL RICH |
7.2 |
3 |
Inoculum control |
7.5 |
4 |
Inoculum control |
7.5 |
5 |
Reference item (procedure control) |
7.8 |
Cumulative Biochemical Oxygen Demand (mg O2/L) in Test Flasks during the Test Period of 60 Days
Time |
Flask No. |
|||||||
(days) |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
|
1 |
0 |
0 |
0 |
0 |
60 |
0 |
60 |
|
2 |
5 |
5 |
5 |
0 |
85 |
0 |
85 |
|
3 |
5 |
10 |
5 |
5 |
105 |
0 |
100 |
|
4 |
20 |
15 |
5 |
5 |
120 |
0 |
120 |
|
5 |
25 |
35 |
5 |
5 |
130 |
0 |
130 |
|
6 |
30 |
45 |
10 |
5 |
135 |
0 |
135 |
|
7 |
40 |
65 |
10 |
10 |
140 |
0 |
145 |
|
8 |
55 |
75 |
10 |
10 |
145 |
0 |
150 |
|
9 |
70 |
85 |
10 |
10 |
150 |
0 |
160 |
|
10 |
85 |
95 |
15 |
10 |
150 |
0 |
160 |
|
11 |
90 |
105 |
15 |
10 |
150 |
0 |
170 |
|
12 |
95 |
115 |
15 |
10 |
155 |
0 |
175 |
|
13 |
100 |
120 |
15 |
10 |
155 |
0 |
180 |
|
14 |
110 |
130 |
20 |
10 |
160 |
0 |
190 |
|
15 |
115 |
140 |
20 |
10 |
160 |
0 |
195 |
|
16 |
120 |
145 |
20 |
15 |
160 |
0 |
200 |
|
17 |
120 |
155 |
25 |
15 |
165 |
0 |
205 |
|
18 |
125 |
160 |
25 |
15 |
165 |
0 |
210 |
|
19 |
130 |
170 |
25 |
20 |
165 |
0 |
215 |
|
20 |
135 |
175 |
25 |
20 |
170 |
0 |
225 |
|
21 |
140 |
185 |
25 |
20 |
170 |
0 |
230 |
|
22 |
140 |
190 |
25 |
20 |
170 |
0 |
240 |
|
23 |
150 |
195 |
25 |
20 |
170 |
0 |
245 |
|
24 |
150 |
200 |
25 |
20 |
170 |
0 |
250 |
|
25 |
155 |
205 |
25 |
20 |
170 |
0 |
250 |
|
26 |
160 |
205 |
25 |
20 |
175 |
0 |
260 |
|
27 |
160 |
210 |
25 |
20 |
175 |
0 |
265 |
|
28 |
165 |
215 |
25 |
20 |
175 |
0 |
275 |
|
29 |
170 |
220 |
25 |
20 |
180 |
0 |
285 |
|
30 |
170 |
220 |
25 |
20 |
180 |
0 |
285 |
|
31 |
170 |
225 |
25 |
20 |
180 |
0 |
285 |
|
32 |
175 |
225 |
25 |
20 |
180 |
0 |
285 |
33 |
175 |
230 |
25 |
20 |
180 |
0 |
285 |
34 |
175 |
230 |
25 |
20 |
180 |
0 |
285 |
35 |
175 |
235 |
25 |
20 |
185 |
0 |
290 |
36 |
175 |
240 |
25 |
20 |
185 |
0 |
290 |
37 |
180 |
245 |
25 |
20 |
190 |
0 |
290 |
38 |
180 |
245 |
25 |
20 |
190 |
0 |
290 |
39 |
180 |
245 |
25 |
20 |
190 |
0 |
290 |
40 |
185 |
245 |
25 |
20 |
190 |
0 |
290 |
41 |
185 |
245 |
25 |
20 |
190 |
0 |
290 |
42 |
190 |
245 |
25 |
20 |
190 |
0 |
290 |
43 |
190 |
250 |
25 |
20 |
190 |
0 |
290 |
44 |
190 |
250 |
25 |
20 |
195 |
0 |
290 |
45 |
190 |
250 |
30 |
20 |
195 |
0 |
290 |
46 |
195 |
255 |
30 |
20 |
195 |
0 |
290 |
47 |
195 |
255 |
30 |
20 |
195 |
0 |
290 |
48 |
195 |
255 |
30 |
20 |
195 |
0 |
290 |
49 |
195 |
255 |
30 |
20 |
195 |
0 |
290 |
50 |
195 |
255 |
30 |
20 |
195 |
0 |
290 |
51 |
200 |
260 |
30 |
20 |
195 |
0 |
290 |
52 |
200 |
260 |
30 |
20 |
195 |
0 |
290 |
53 |
200 |
260 |
30 |
20 |
195 |
0 |
290 |
54 |
200 |
260 |
30 |
20 |
195 |
0 |
290 |
55 |
200 |
260 |
30 |
20 |
195 |
0 |
290 |
56 |
200 |
260 |
30 |
20 |
195 |
0 |
290 |
57 |
205 |
260 |
30 |
20 |
195 |
0 |
290 |
58 |
205 |
260 |
30 |
20 |
195 |
0 |
290 |
59 |
210 |
260 |
30 |
20 |
200 |
0 |
295 |
60 |
210 |
260 |
30 |
20 |
200 |
0 |
295 |
Flasks 1 and 2: PATCHOULI EO FRACTION PATCHOULOL RICH
Flasks 3 and 4: inoculum control
Flask5: reference(procedure control)
Flask 6: abiotic control
Flask 7: toxicity control
Percentage Biodegradation of Test Item, of Sodium Benzoate and of the Toxicity Control based on ThODNH4:
Time |
Percentage Biodegradation1 |
|||
|
|
|||
(Days) |
PATCHOULI EO FRACTION PATCHOULOL RICH1 |
Sodium Benzoate2 |
Toxicity control1, 2 |
|
|
Flask 1 [%] |
Flask 2 [%] |
Flask 5 [%] |
Flask 7 [%] |
1 |
0 |
0 |
35 |
13 |
2 |
1 |
1 |
48 |
17 |
3 |
0 |
2 |
59 |
20 |
4 |
5 |
3 |
67 |
24 |
5 |
7 |
10 |
73 |
26 |
6 |
7 |
12 |
75 |
27 |
7 |
10 |
18 |
76 |
28 |
8 |
15 |
21 |
79 |
29 |
9 |
20 |
24 |
82 |
32 |
10 |
24 |
26 |
81 |
31 |
11 |
25 |
30 |
81 |
33 |
12 |
27 |
33 |
83 |
34 |
13 |
29 |
34 |
83 |
35 |
14 |
31 |
37 |
85 |
37 |
15 |
33 |
40 |
85 |
38 |
16 |
34 |
41 |
83 |
38 |
17 |
33 |
43 |
85 |
39 |
18 |
34 |
45 |
85 |
40 |
19 |
35 |
47 |
83 |
41 |
20 |
37 |
49 |
86 |
43 |
21 |
38 |
52 |
86 |
44 |
22 |
38 |
53 |
86 |
46 |
23 |
42 |
55 |
86 |
47 |
24 |
42 |
57 |
86 |
48 |
25 |
43 |
58 |
86 |
48 |
26 |
45 |
58 |
89 |
50 |
27 |
45 |
60 |
89 |
51 |
28 |
47 |
61 |
89 |
53 |
29 |
48 |
63 |
92 |
55 |
30 |
48 |
63 |
92 |
55 |
31 |
48 |
65 |
92 |
55 |
32 |
50 |
65 |
92 |
55 |
33 |
50 |
66 |
92 |
55 |
34 |
50 |
66 |
92 |
55 |
35 |
50 |
68 |
95 |
56 |
36 |
50 |
69 |
95 |
56 |
37 |
52 |
71 |
98 |
56 |
38 |
52 |
71 |
98 |
56 |
39 |
52 |
71 |
98 |
56 |
40 |
53 |
71 |
98 |
56 |
41 |
53 |
71 |
98 |
56 |
42 |
55 |
71 |
98 |
56 |
43 |
55 |
73 |
98 |
56 |
44 |
55 |
73 |
101 |
56 |
45 |
54 |
72 |
100 |
56 |
46 |
56 |
73 |
100 |
56 |
47 |
56 |
73 |
100 |
56 |
48 |
56 |
73 |
100 |
56 |
49 |
56 |
73 |
100 |
56 |
50 |
56 |
73 |
100 |
56 |
51 |
57 |
75 |
100 |
56 |
52 |
57 |
75 |
100 |
56 |
53 |
57 |
75 |
100 |
56 |
54 |
57 |
75 |
100 |
56 |
55 |
57 |
75 |
100 |
56 |
56 |
57 |
75 |
100 |
56 |
57 |
59 |
75 |
100 |
56 |
58 |
59 |
75 |
100 |
56 |
59 |
60 |
75 |
103 |
57 |
60 |
60 |
75 |
103 |
57 |
1ThODNH4of
PATCHOULI EO FRACTION PATCHOULOL RICH: 3.021 mg O2/mg test
item
2ThODNH4of
sodium benzoate: 1.666 mg O2/mg reference item
Biodegradation in the Toxicity Control
Percentage Biodegradation: |
In the toxicity control containing both, the test item and the reference item sodium benzoate, 37% (ThODNH4) biodegradation was noted within 14 days, 53% (ThODNH4) biodegradation after 28 days and 57% (ThODNH4) after 60 days of incubation. |
Conclusion: |
According to the test guidelines the test item can be assumed to be not inhibitory to the aerobic activated sludge microorganisms because degradation was >25% within 14 days. |
Abiotic Control
Oxygen Demand: |
The oxygen demand in the abiotic control was 0 mg/L during the test duration. There was no need to correct the degradation of the test item and toxicity control. |
Validity criteria
Inoculum Control: |
The oxygen demand of the inoculum control (medium and inoculum) was 22.5 mg O2/L and thus not greater than 60 mg O2/L within 28 days as required by the test guideline. |
|
pH-Value: |
The pH-value of the test item flasks at day 63 was 7.2 to 7.3 and therefore within the range of pH 6.0 to 8.5 as required by the test guideline. |
|
Reference Item: |
The percentage degradation of the reference item should reach the level for ready biodegradability (>60%) within 14 days as required by the test guideline. The reference item sodium benzoate was degraded to more than 60% after 4 days of incubation. |
|
Test Item: |
The difference of duplicate values for the degradation of the test item at the end of the 10-day window, at day 28 and at day 60 of the test was less than 20%. The difference of duplicate values at days 17, 28 and 60 differed by 10%, 14% and 5%, respectively. The validity criterion was fulfilled. |
|
Toxicity Control: |
If in a toxicity test, containing both the test item and a reference item less than 25% biodegradation (based on ThODNH4) occurred within 14 days, the test item can be assumed to be inhibitory. The biodegradation was 37% at day 14; the test item was not inhibitory. |
|
Description of key information
With a mean biodegradation rate (ThOD-NH4) < 60% after 28 days of incubation, the test item is considered not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
The test item Patchouli EO Fraction Patcoulol Rich was investigated for its ready biodegradability in a manometric respirometry test, according to the OECD 301F guideline and under GLP compliance. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
The mean biodegradation of 10% of PATCHOULI EO FRACTION PATCHOULOL RICH was reached at day 7 (ThODNH4). At the end of the 10-day window at day 17, the mean degradation of PATCHOULI EO FRACTION PATCHOULOL RICH was 38% (ThODNH4).The mean biodegradation after 28 days was 54% (ThODNH4) and 68% (ThODNH4) after 60 days.
Therefore, the test item is considered not readily biodegradable according to test guideline.
The reference item sodium benzoate was sufficiently degraded to 67% after 14 days and to 89% after 28 days of incubation, thus confirming the suitability of the aerobic activated sludge inoculum used.
In the toxicity control containing both, the test item and the reference item sodium benzoate, 37% biodegradation was noted within 14 days and 53% biodegradation after 28 days of incubation. According to the test guidelines, the test item can be assumed to be not inhibitory to the aerobic activated sludge microorganisms because degradationwas >25% within 14 days.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.