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Reaction mass of trisodium [m[7-[[4'-[[6-benzamido-1-hydroxy-3-sulpho-2-naphthyl]azo]-3,3'-dihydroxy[1,1'-biphenyl]-4-yl]azo]-8-hydroxynaphthalene-1,6-disulphonato(7-)]]dicuprate(3-) and trisodium [m[4-[[4'-[[6-benzamido-1-hydroxy-3-sulpho-2-naphthyl]azo]-3,3'-dihydroxy[1,1'-biphenyl]-4-yl]azo]-3-hydroxynaphthalene-2,7-disulphonato(7-)]]dicuprate(3-)
EC number: 947-266-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From October 28th to December 20th, 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- April 4, 1984
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Concentrations were measured at the beginning and at the end of the experiment.
- Vehicle:
- no
- Details on test solutions:
- The test solutions were prepared by adding approximate amounts of a stock solution of the test material to test water, keeping the volume of the test solution at 4 litres in each test vessel.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Name: zebrafish, Brachydanio rerio, Hamilton-Buchanan.
- Source: Stubenvoll, Allschwil, Switzerland.
- Length at study initiation: 3 - 4 cm
- Weight at study initiation: 0.57 g
ACCLIMATION
- Acclimation period: acclimatization for a minimum of 12 days in 100 liter glass tanks.
- Acclimation conditions: areated test water of 23 ± 2 °C, under flow-through conditions.
- Feeding: fed daily, until 24 hours before starting the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 22 - 23 °C
- pH:
- 7.2 - 8.0
- Dissolved oxygen:
- 5.4 - 8.6 mg/l, i.e. 60 - 95 % saturation
- Nominal and measured concentrations:
- 100, 180, 320, 560 and 1000 mg/l (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass tanks (150 x 200 x 200 mm) with 4 l test solution.
- Aeration: none.
- No. of organisms per concentration: 10 fish.
- No. of vessels per concentration: 2 replicate.
- No. of vessels per control: 1 replicate.
- Biomass loading rate: 5 fish per 4 litres test solution, i.e. 0.71 g fish/l.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: carbon filtered drinking water.
- Total hardness: about 180 mg CaCO3/l.
- pH: 7.6 to 8.0.
OTHER TEST CONDITIONS
- Light: fluorescent lighting.
MEASUREMENTS
Dissolved oxygen, pH and temperature were measured daily in each test vessel. - Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- No deaths occurred over the 96-hours study period at none of the tested concentrations.
- Sublethal observations / clinical signs:
Measured concentrations
Nominal concentration (mg/l) At the beginning of the test, DOC (mg/l) At the end of the test, DOC (mg/l) At the end of the test, % of DOC at beginning Control 3.5 5 - Test item 100 47 27 50.5 Test item 180 69 58 81 Test item 320 132.5 105 78 Test item 560 225 155 68 Test item 1000 395 305 77 Test conditions
O2 (mg/l) 0 hrs 24 hrs 48 hrs 72 hrs 96 hrs Control 8.6 7.8 7.4 7.6 7.7 Test item 100 8.1 8.2 7.4 5.8 6.1 Test item 180 7.9 8.4 7.3 5.8 5.9 Test item 320 7.5 8.1 7.6 6.7 6.7 Test item 560 7.0 8.1 7.1 7.0 6.9 Test item 1000 5.4 7.7 6.6 6.8 7.1 % oxygen saturation 0 hrs 24 hrs 48 hrs 72 hrs 96 hrs Control 95 86 82 84 84 Test item 100 90 89 80 62 67 Test item 180 88 90 79 63 64 Test item 320 83 88 82 72 73 Test item 560 77 87 76 76 74 Test item 1000 60 82 71 74 77 pH 0 hrs 24 hrs 48 hrs 72 hrs 96 hrs Control 7.2 7.7 7.9 8.0 8.0 Test item 100 7.3 7.8 7.9 7.6 7.8 Test item 180 7.2 7.9 7.9 7.7 7.7 Test item 320 7.3 7.9 8.0 7.9 7.9 Test item 560 7.2 7.8 7.9 7.9 7.9 Test item 1000 7.2 7.8 7.9 7.9 8.0 Temperature (°C) 0 hrs 24 hrs 48 hrs 72 hrs 96 hrs Control 23 23 23 23 23 Test item 100 23 22 22 22 22 Test item 180 23 22 22 22 22 Test item 320 23 22 22 22 22 Test item 560 23 22 22 22 22 Test item 1000 23 22 22 22 22 - Conclusions:
- LC50 (96h) > 1000 mg/l (nominal, corresponding to ca 770 mg/l, based on DOC measurement).
- Executive summary:
The potential for acute toxicity to fish of the test item was investigated in zebrafish (Brachydanio rerio, Hamilton-Buchanan). Fish were tested in a static experiment, conducted over a period of 96 hours. Stability of test item during the experimental period was checked measuring the DOC vessel content, at the beginning and at the end of the experiment. The percentage of the DOC amount at the end of the test respect to the quantity of the beginning was 50.5, 81, 78, 68 and 77 %, corresponding to the nominal cocnetration of 100, 180, 320, 560 and 1000 mg/l, respectively.
No deaths occurred over the 96-hours study period at none of the tested concentrations.
Conclusion
LC50 (96h) > 1000 mg/l (nominal, corresponding to ca 770 mg/l, based on DOC measurement).
Reference
Description of key information
Non harmful/toxic for short-term exposures to fish. LC50 (96h) higher than 1000 mg/l (nominal, corresponding to ca 770 mg/l, based on DOC measurement).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 770 mg/L
Additional information
The potential for acute toxicity to fish of the test item was investigated in zebrafish (Brachydanio rerio, Hamilton-Buchanan). Fish were tested in a static experiment, conducted over a period of 96 hours. Stability of test item during the experimental period was checked measuring the DOC vessel content, at the beginning and at the end of the experiment. The percentage of the DOC amount at the end of the test respect to the quantity of the beginning was 50.5, 81, 78, 68 and 77 %, corresponding to the nominal cocnetration of 100, 180, 320, 560 and 1000 mg/l, respectively. No deaths occurred over the 96-hours study period at none of the tested concentrations.
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