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Diss Factsheets
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EC number: 601-264-5 | CAS number: 113534-44-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 Sep 1983 - 24 Jun 1984
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
- Report date:
- 1984
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- no
- Test type:
- traditional method
- Limit test:
- no
Test material
- Reference substance name:
- 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1)
- EC Number:
- 264-355-5
- EC Name:
- 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1)
- Cas Number:
- 63589-47-9
- Molecular formula:
- C20H26N3O.1/2Cl4Zn
- IUPAC Name:
- bis[3,7-bis(diethylamino)phenoxazin-5-ium] tetrachlorozincate(2-)
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Dr. K. Thomae GmbH, Biberach, Germany
- Age at study initiation: 8 wks
- Weight at study initiation: 253 +/- 20 g (male), 178 +/- 16 g (female)
- Housing: Metal cages type D IIII (5 animals / cage)
- Diet (e.g. ad libitum): ad lib.
- Water (e.g. ad libitum): ad lib.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 24°C
- Humidity (%): 30 - 70% rh
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- inhalation: dust
- Type of inhalation exposure:
- nose/head only
- Vehicle:
- clean air
- Mass median aerodynamic diameter (MMAD):
- 3.5 µm
- Geometric standard deviation (GSD):
- 2.6
- Remark on MMAD/GSD:
- 88% of inhalation particles are fine particulates
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Head/nose inhalation system INA 20 (BASF)
- Exposure chamber volume: 55 L
- Method of holding animals in test chamber: rats are sitting in tubes and have their head in the inhalation chamber
- Source and rate of air: 1500 L/h clean air
- Method of conditioning air: centralised climatisation apparatus
- System of generating particulates/aerosols: vibration dosing apparatus
- Method of particle size determination: Impaktor analysis, gravimetry
- Temperature, humidity, pressure in air chamber: ambient
TEST ATMOSPHERE
- Brief description of analytical method used: gravimetry
- Samples taken from breathing zone: yes
TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: up to 29.5 µm (4.1%), 18.2 µm (2.7%), 8.5 µm (12.2%), 5.5 µm (15.5%), 2.8 µm (43.6%), 1.2 µm (16.8%), <1.2 µm (5.1%)
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): 3.5 / 2.6 = 1.35 - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- gravimetry
- Duration of exposure:
- 4 h
- Concentrations:
- 0.51, 2.15, 5.20 mg/L
- No. of animals per sex per dose:
- 10
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observations daily; weighing on day 0, 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Statistics:
- binomial test
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 0.51 - < 2.15 mg/L air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- 0.51 mg/L: 0% mortality
2.15 mg/L: 100% mortality after 7 days
5.20 mg/L: 100% mortality after 2 hours - Clinical signs:
- other: 0.51 mg/L: day 1 irregular breathing noises 2.15 mg/L: eyelid closure, intermittent breath, attempts to escape, crouch position, staggering, bristled fur 5.20 mg/L: continuous attempts to escape at beginning, 100% mortality after 2 hours
- Body weight:
- 0.15 mg/L: no effects on body weight gain compared to control
- Gross pathology:
- Dead animals:
- lungs: hyperemia, edema, pneumonic areas
- liver: anemia, speckled greyish brown areas
- nephrons: ischemia tubulonecrosis, protein filled tubuli
Sacrificed animals: no findings
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- The substance caused 100% mortality in dust concentrations of 2.15 and 5.20 mg/L air in male and female rats. The 4-h LC50 (inhalation) was determined between 0.51 and 2.15 mg/L air. Therefore, the substance is classified as Category 3 for acute inhalation toxicity.
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