Registration Dossier
Registration Dossier
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EC number: 419-210-2 | CAS number: 178452-71-6 OLIVE-GREEN JB 1170
Endpoint summary
Administrative data
Description of key information
Short-term toxicity to fish:
LC50 (96h) = 54 mg/L
Short-term toxicity to aquatic invertebrates:
EC50 (48h)= 62 mg/L
Toxicity to aquatic algae:
ErC50 > 100 mg/L
Toxicity to microorganisms:
EC50 > 100 mg/L
Additional information
It is worth saying that the substance is very water soluble (i.e. > 10 g/l); it has a log Kow lower than 4 (indicating a low potential for bioaccumulation) and it is not rapidly degradable.
The short-term toxicity to fish was investigated to Oncorhynchus mykiss, according to the OECD Guideline 203 (1992) and the method C.1 of EEC Directive 92/69 EEC. The LC50 (96h) was found to be 54 mg/L.
The short-term toxicity to invertebrates was investigated to Daphnia magna, according to the OECD Guideline 202, Part I (1984) and to the method C.2 of the EEC-Directive 92/69. The EC50 (48 h) was found to be 62 mg/L.
Toxicity to aquatic algae was investigated to Scenedesmus subspicatus, according to the OECD Guideline 201 (1984) and the method C.3 of EEC-Directive 92/69 EEC. The test method was modified to differentiate between a reduced growth of algae due to real toxic effects of the test substance on the algal cells or due to an indirect effect, a reduced algal growth by light absorption in coloured test solutions. The ErC50 (72 h) for a real toxic effect was found to be greater than 100 mg/L.
Furthermore, microorganisms (activated domestic water sludge) was investigated. The EC50 was found to be higher than 100 mg/L.
Justification for classification or non-classification
Adequate chronic toxicity data are not available and the substance is not rapidly degradable; therefore the aquatic toxicity assessment and classification are based mainly on the short-term toxicity data available on the substance. According to the CLP Regulation (EC 1272/2008), acute aquatic toxicity is normally determined using a fish 96-hour LC50, a crustacean species 48-hour EC50 and/or an algal species 72- or 96-hour EC50.
The acute toxicity tests to fish and to invertebrates fixed the effect levels within the range > 10 to≤100 mg/L. Therefore, the substance is classified as Category Chronic 3 according to the CLP Regulation (EC 1272/2008).
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