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EC number: 265-016-4 | CAS number: 64683-40-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- other: Real Decreto 363/1995 (BOE núm. 133 de Junio de 1995)
- GLP compliance:
- no
Test material
- Reference substance name:
- 7-amino-4-hydroxy-3-[[4-[(4-sulphophenyl)azo]phenyl]azo]naphthalene-2-sulphonic acid, compound with 2,2',2''-nitrilotriethanol (1:2)
- EC Number:
- 265-016-4
- EC Name:
- 7-amino-4-hydroxy-3-[[4-[(4-sulphophenyl)azo]phenyl]azo]naphthalene-2-sulphonic acid, compound with 2,2',2''-nitrilotriethanol (1:2)
- Cas Number:
- 64683-40-5
- Molecular formula:
- C22H17N5O7S2.2C6H15NO3
- IUPAC Name:
- 7-amino-4-hydroxy-3-({4-[(4-sulfophenyl)diazenyl]phenyl}diazenyl)naphthalene-2-sulfonic acid - 2,2',2''-nitrilotriethanol (1:2)
- Test material form:
- solid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Secondary effluent from the Urban Water Treatment Plant in Manresa (Spain)
No further details on inoculum given in the study report. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 5 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Solution 1:
KH2PO4 8.5 g/L H2O
K2HPO4 21.75 g/L H2O
Na2HPO4 2H2O 33.4g /L H2O
NH4Cl 0.5 g/L H2O
Solution 2: CaCl 2 2H2O 36.4 g/L H2O
Solution 3: MgSO 47H2O 22.5 g /L H2O
Solution 4: FeCl3 6H2O 0.25 g/L H2O
Add to 1000 mL of deionized water:
Solution 1 1 mL
Solution 2 1 mL
Solution 3 1 mL
Solution 4 1 mL
- Test temperature: 20 ± 2 ºC
- pH: not specified
- Aeration of dilution water: not specified
- Suspended solids concentration: not specified
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Erlenmeyer flasks (100 ml) with a cap, fully filled
- Number of culture flasks/concentration: 2
- Measuring equipment: not specified
- Details of trap for CO2 and volatile organics if used: not specified
- Other:
Preparation of stock and test solutions: A stock solution having a concentration of 1 g/L of the material presented (ref: Red P / Paper 8BLP (batch: PAL-025777)) was prepared. Therefore, 0.1 g of the presented material has been dissolved in 100 mL of water in a 100 mL Class A volumetric flask by shaking, so we have a stock solution of 1000 mg / L. To prepare the solution to be tested (5 mg / L), 10 mL of stock solution was diluted in 2 liters of inoculated culture medium.
The test containers have been filled with the solution to overflow, so that no bubbles of air remain inside the bottle.
Concentrations per test: 1 + substance for reference + blank
SAMPLING
- Sampling frequency: at day 0, 3, 7, 10, 14, 21, 28 (Number of measurements: 7)
- Sampling method: not specified
- Sterility check if applicable: not specified
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: not specified
- Toxicity control: not specified
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 0 h
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0.9
- Sampling time:
- 3 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 2.3
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 6
- Sampling time:
- 10 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 11.1
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 13.7
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 18
- Sampling time:
- 28 d
BOD5 / COD results
BOD5 / COD
- Parameter:
- COD
- Value:
- 1.74 g O2/g test mat.
- Results with reference substance:
- Sodium acetate: 0,68 mg O2/mg
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The result obtained in this study indicates that the test item has a biodegradability of 18% at the initial concentration tested (5 mg / L) at 28 days of testing.
- Executive summary:
The determination of the biodegradability has been carried out after 28 days for the test substance DR 254 TEA salt following the OECD Guideline 301D (closed bottle test) (1992) together with the Spanish Royal Decree 363/1995 (BOE No. 133 of June 1995).
Biodegradation is defined as the decomposition of an organic compound by the biological action of microorganisms, which results in the disappearance of the original molecular structure and the formation of smaller organic molecules.The results are expressed as the percentage of biodegradation after 28 days.
Starting from a known concentration of the test substance (5 mg / L), monitoring is carried out by measuring the oxygen consumption at intervals of time over a period of 28 days.
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