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EC number: 256-155-1 | CAS number: 43229-65-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 22 April to 26 July 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N-benzyl-4-methoxy-α-methylphenethylamine
- EC Number:
- 256-155-1
- EC Name:
- N-benzyl-4-methoxy-α-methylphenethylamine
- Cas Number:
- 43229-65-8
- Molecular formula:
- C17H21NO
- IUPAC Name:
- N-benzyl-1-(4-methoxyphenyl)propan-2-amine
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): TH 1165 II
- Density: 1.05 g/ml (determined at NOTOX)
- Physical state: Yellow liquid
- Analytical purity: 98.2 % (area)
- Lot/batch No.: 61
- Expiration date of the lot/batch: 8 April 2000 (allocated by NOTOX, 1 year after receipt of the test substance)
- Stability under test conditions: Not indicated
- Storage condition of test material: At room temperature in the dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The standard test procedures required generation of test solutions containing completely dissolved test substance concentrations or stable and homogeneous mixture or dispersions. The testing of concentrations that would disturb the test system was prevented (e. g. film of the test substance on the water surface).
Preparation started with a stock solution at 100 mg/l. Treatment with ultrasonic waves for 30 minutes was applied to dissolve the test substance in the test medium. The various test concentrations were prepared by subsequent dilutions of the the 100 mg/l stock in test medium. The final test solutions were all clear and colourless.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (Crustacea, Cladocera)
- Age at study initiation (mean and range, SD): < 24 hours
- Method of breeding: The daphnia were bred with new-born animals, i. e. less than 3 days old, by placing about 250 of them into 10 litres of medium in an all-glass culture vessel. The maximum age of the cultures were 4 weeks. The renewal of the cultures were performed after 7 days of cultivation half of the medium twice a week. The temperature of the medium was hold at 18 - 22°C. Feeding was done daily, a suspension of fresh water algae. Breeding was performed in a M7medium, as prescribed by Dr. Elendt-Schneider (Elendt, B.-P., 1990)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- no data
- Test temperature:
- The temperature of the test medium measured in the blank control varied from 20.9 to 21.6ºC.
- pH:
- 8.5
- Dissolved oxygen:
- 8.93 mg O2/L
- Salinity:
- not applicable
- Nominal and measured concentrations:
- nominal concentration: 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml, all-glass
- Aeration: No aeration of the test solutions
- No. of organisms per vessel: 20 per concentration and 10 per vessel containing 80 ml medium
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2 blank control
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO medium, prepared in milli-RO water
OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobility (including mortality) at 24 hours and 48 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 for the range finding test
- Range finding study: 0.1, 1.0, 10, 100 mg/l of the test substance
- Test concentrations: 2.2, 4.6, 10, 22, 46 mg/l of the test substance - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.9 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.1 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mobility
Any other information on results incl. tables
Acute immobilisation of daphnia after 24 and 48 hours in the final EC50 -test:
Concentration TH 1165 II (mg/l) | Vessel number | Number Daphnia exposed | Response at 24 h | Response at 48 h | ||
number | % | number | % | |||
Blank-control | A | 10 | 0 | 0 | 0 | 0 |
B | 10 | 0 | 0 | 0 | 0 | |
2.2 | A | 10 | 0 | 0 | 0 | 0 |
B | 10 | 0 | 0 | 0 | 0 | |
4.6 | A | 10 | 2 | 20 | 3 | 30 |
B | 10 | 1 | 10 | 6 | 60 | |
10 | A | 10 | 8 | 80 | 9 | 90 |
B | 10 | 10 | 100 | 10 | 100 | |
22 | A | 10 | 10 | 100 | 10 | 100 |
B | 10 | 10 | 100 | 10 | 100 | |
46 | A | 10 | 10 | 100 | 10 | 100 |
B | 10 | 10 | 100 | 10 | 100 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study, TH 1165 II did not induce acute immobilisation of Daphnia Magna at 2.2 mg/l after 48 hours of exposure (NOEC).
The 24h-EC50 was 6.1 mg/l with a 95% confidence interval between 5.2 and 7.5 mg/l.
The 48h-EC50 was 4.9 mg/l with a 95% confidence interval between 4.3 and 6.0 mg/l.
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