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EC number: 211-806-9 | CAS number: 697-82-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- The toxicity of test substance 2,3,5-trimethylphenol was determined with a Microtox model 500 toxicity autoanalyzer (Microbics Corp., Carlsbad, Calif.)
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 2,3,5Trimethylphenol
- Molecular formula (if other than submission substance): C9H12O
- Molecular weight (if other than submission substance): 136.193 g/mol
- Smiles notation (if other than submission substance): c1(c(cc(C)cc1O)C)C
- InChl (if other than submission substance): 1S/C9H12O/c1647(2)8(3)9(10)56/h45,10H,13H3
- Substance type: Organic
- Physical state: Solid - Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:Each flask containing 25 ml of GWM was inoculated with 1.0 ml (27 pLg of bacterial protein) of the soil microbial suspension. - Test organisms (species):
- other: "creosote-adapted" microorganisms
- Details on inoculum:
- Microbial inoculum was prepared by mixing 25 g of creosote-contaminated surface soil freshly obtained from the American Creosote Works site with 100 ml of 2.5 mM phosphate buffer (pH 7). After being mixed well, the suspension was centrifuged (2,500 rpm, 10 min) to remove larger soil particles. The resultant supernatant was decanted and used as a source of indigenous, "creosote-adapted"microorganisms for the groundwater medium (GWM)
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 14 d
- Hardness:
- No data
- Test temperature:
- 30°C
- pH:
- 7
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Conductivity:
- No data
- Details on test conditions:
- TEST SYSTEM
- Test vessel:125-ml Erlenmeyer flasks fitted with Teflon-lined screw caps
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume:12.5 ml
OTHER TEST CONDITIONS
- Adjustment of pH:adjusted to pH 12.0 with 1 N NaOH - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 7 000 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- other: measuring the change in light level of viable luminescent bacteria upon their exposure to test substrates.
- Remarks on result:
- other: Microtox assays demonstrated a 50% effective concentration (EC50) of 0.72 (a solution containing 0.72% parent material killed 50% of the test organisms) 0.72% cocentration equivalent to 7000 mg/l
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Microtox assays demonstrated a 50% effective concentration (EC50) of 0.72 (a solution containing 0.72% parent material killed 50% of the test organisms) this 0.72% concentration equivalent to 7000 mg/l of test substance 2,3,5-trimethylphenol.
- Executive summary:
The toxicity of test substance 2,3,5-trimethylphenol was determined with a Microtox model 500 toxicity autoanalyzer (Microbics Corp., Carlsbad, Calif.)
Microbial inoculum was prepared by mixing 25 g of creosote-contaminated surface soil freshly obtained from the American Creosote Works site with 100 ml of 2.5 mM phosphate buffer (pH 7). After being mixed well, the suspension was centrifuged (2,500 rpm, 10 min) to remove larger soil particles. The resultant supernatant was decanted and used as a source of indigenous, "creosote-adapted"microorganisms for the groundwater medium (GWM).Each flask containing 25 ml of GWM was inoculated with 1.0 ml (27 pLg of bacterial protein) of the soil microbial suspension.
The Microtox assays demonstrated a 50% effective concentration (EC50) of 0.72 (a solution containing 0.72% parent material killed 50% of the test organisms) this 0.72% concentration equivalent to 7000 mg/l of test substance 2,3,5-trimethylphenol.
Reference
Description of key information
The toxicity of test substance 2,3,5-trimethylphenol was determined with a Microtox model 500 toxicity autoanalyzer (Microbics Corp., Carlsbad, Calif.)
Microbial inoculum was prepared by mixing 25 g of creosote-contaminated surface soil freshly obtained from the American Creosote Works site with 100 ml of 2.5 mM phosphate buffer (pH 7). After being mixed well, the suspension was centrifuged (2,500 rpm, 10 min) to remove larger soil particles. The resultant supernatant was decanted and used as a source of indigenous, "creosote-adapted"microorganisms for the groundwater medium (GWM).Each flask containing 25 ml of GWM was inoculated with 1.0 ml (27 pLg of bacterial protein) of the soil microbial suspension.
The Microtox assays demonstrated a 50% effective concentration (EC50) of 0.72 (a solution containing 0.72% parent material killed 50% of the test organisms) this 0.72% concentration equivalent to 7000 mg/l of test substance 2,3,5-trimethylphenol.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 7 000 mg/L
Additional information
Three studies including experimental data from peer reviewed journals for toxicity to micro organism endpoint of test chemical 2, 3, 5-trimethylphenol (Cas no. 697-82-5) with relevant read across which is structurally similar to target were summarized as followed:
First experimental study (APPLIED AND ENVIRONMENTAL MICROBIOLOGY, May 1991, p. 1277-1285)suggest the toxicity of test substance 2,3,5-trimethylphenol was determined with a Microtox model 500 toxicity autoanalyzer (Microbics Corp., Carlsbad, Calif.) Microbial inoculum was prepared by mixing 25 g of creosote-contaminated surface soil freshly obtained from the American Creosote Works site with 100 ml of 2.5 mM phosphate buffer (pH 7). After being mixed well, the suspension was centrifuged (2,500 rpm, 10 min) to remove larger soil particles. The resultant supernatant was decanted and used as a source of indigenous, "creosote-adapted"microorganisms for the groundwater medium (GWM).Each flask containing 25 ml of GWM was inoculated with 1.0 ml (27 pLg of bacterial protein) of the soil microbial suspension. The Microtox assays demonstrated a 50% effective concentration (EC50) of 0.72 (a solution containing 0.72% parent material killed 50% of the test organisms) this 0.72% concentration equivalent to 7000 mg/l of test substance 2,3,5-trimethylphenol.
And one another peer reviewed journal Chemical Research in Toxicology; vol. 12; nb. 12; (1999); p. 1262 – 1267 for target chemical indicate Population growth impairment testing with the ciliate T. pyriformis (strain GL-C) was conducted following the protocol described by Schultz of test substance 2,3,5-trimethylphenol.
The test protocol allows for eight to nine cell cycles in controls. Following range finding, each chemical was tested in three replicate tests (or assays). Two controls were used to provide a measure of the acceptability of the test by indicating the suitability of the medium and test conditions as well as a basis for interpreting data from other treatments. The first control had no test material and was inoculated with T. pyriformis. The other, a blank, had neither test material nor ciliates. Test replicate consisted of six to eight different concentrations.The IGC50 is calculated by Structure-toxicity models using the regression procedure of MINITAB version 12.1.
A test on growth impairment with Tetrahymena pyriformis results in an inhibitory growth concentration (IGC50) of 1/log IGC50=0.36 which is equivalent to 599.48 (dimensionless) after 40 hours.
Whereas read across chemical 2,3,6 Trimethylphenol (CAS no. 2416-94-6) suggest Inhibition concentration to 50% of test organisms (IGC50) for the substance 2,3,6Trimethylphenol (CAS no. 2416-94-6) was determine to be 52.05 mgl/L on micro organism species Tetrahymena pyriformis (Ciliate) on the basis of effect on Population growth rate in static fresh water.( ECOTOX DATABASE; 2017)
On the basis of available effects values on micro organism from above three studies give the common conclusion as test chemical 2, 3, 5-trimethylphenol (Cas no. 697-82-5) have no concern to micro organism for toxicity in acute exposure period.
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