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EC number: 209-939-2 | CAS number: 598-55-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From May 3, 1982 to June 2, 1982
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to method comparable to OECD Guideline 401, in compliance with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Methyl carbamate
- EC Number:
- 209-939-2
- EC Name:
- Methyl carbamate
- Cas Number:
- 598-55-0
- Molecular formula:
- C2H5NO2
- IUPAC Name:
- methyl carbamate
- Test material form:
- other: Solid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Blue Spruce Farms, Altamont, New York
- Weight at study initiation: 180 - 280 g (after fasting)
- Fasting period before study: 18 h before administration of test substance
- Housing: Rats housed in groups, according to sex, or individually in stainless steel 1/2" wire mesh cages. Size in accordance with the "Guide for the Care and
Use of Laboratory Animals" of the Institute of Laboratory Resources, National Research Council.
- Diet: Wayne Lab Blox, ad libitum
- Water: Fresh tap water, ad libitum
- Acclimation period: 5 d
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22°C±3°C
- Humidity (%): 30 to 70%
- Photoperiod: 12 h dark and 12 h light
IN-LIFE DATES: From May 03, 1982 to May 17, 1982
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: 0.25% methyl cellulose
- Details on oral exposure:
- VEHICLE
- Volume administration: 5 mL/kg and 10 mL/kg
- Rationale for the selection of the starting dose: Based on the results of a dose-range-finding study.
Dose preparation
2,000 mg/kg - 6.0 g q.s. to 15 mL of 0.25% methyl cellulose
2,500 mg/kg - 7.5 g q. s. to 15 mL of 0.25% methyl cellulose
3,000 mg/kg - 9.0 g q. s. to 15 mL of 0.25% methyl cellulose
3,500 mg/kg - 10.5 g q. s. to 30 mL of 0.25% methyl cellulose
4,000 mg/kg - 12.0 g q.s. to 30 mL of 0.25% methyl cellulose
4,500 mg/kg - 12.1 g q. s. to 26.9 mL of 0.25% methyl cellulose - Doses:
- 2,000, 2,500, 3,000, 3,500, 4,000 and 4,500 mg/kg bw
- No. of animals per sex per dose:
- 5/sex/dose
- Details on study design:
- - Duration of observation period following administration: 14 d
- Frequency of observations: The rats were observed immediately, 1 h and 4 h after dosing and daily thereafter for pharmacotoxic, CNS effects and mortality.
- Frequency of weighing: Body weights were recorded initially, Day 14 and at time of death.
- Necropsy of survivors performed: Surviving rats were sacrificed by CO2 inhalation and a gross necropsy was performed.
Results and discussion
- Preliminary study:
- Signs observed in dose ranging finding study were abnormal gait, semiprostration, body drop, loss of equilibrium, decreased activity, prostration, decreased body tone, increased body tone, exophthalmus and yellow-brown discoloration around anus. None of the rats died at 500 mg/kg bw, one rat died at 1,600 mg/kg bw and three rats died at the 5,000 mg/kg bw dose level.
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 3 900 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 3 514 - 4 329
- Mortality:
- 2 males died at dose level of 3,000 mg/kg bw.
1 male died at dose level of 3,500 mg/kg bw.
2 males and 4 females died at dose level of 4,000 mg/kg bw.
3 males and 4 females died at dose level of 4,500 mg/kg bw. - Clinical signs:
- other: Body drop, ptosis, decreased activity, labored respiration, chromodacryorrhea, decreased body tone, abnormal gait, abnormal stance, partial paralysis, loss of equilibrium, dried red exudate around nasal cavity, semiprostration, prostration, ataxia, diarrh
- Gross pathology:
- Necropsy of animals dying on study revealed distended, air filled stomachs and intestines, discolored adrenals, lungs, thymus, spleen, small ulcers or erosions of glandular mucosa, hemorrhages in cecum and small amounts of fluid in thoracic cavity. No visible lesions were observed in the remaining animals.
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The acute oral LD50 of the test substance for male and female rats was determined to be ≥3,900 mg/kg bw, with 95% confidence limits of 3,514 to 4,329 mg/kg bw.
- Executive summary:
A study was conducted to assess the acute toxicity of the test substance to rats according to method comparable to OECD Guideline 401, in compliance with GLP. Dose levels were selected on basis of a dose ranging study. In a preliminary dose-range-finding study, three groups of four fasted animals per group, two per sex, were given the test substance at dose levels of 500, 1,600 and 5,000 mg/kg bw orally, by gavage. Signs observed were abnormal gait, semiprostration, body drop, loss of equilibrium, decreased activity, prostration, decreased body tone, increased body tone, exophthalmus and yellow-brown discoloration around the anus. None of the rats died at 500 mg/kg bw, one of four died at 1,600 mg/kg bw and three of the animals died at the 5,000 mg/kg bw dose level. In main study, the test substance was administered to males and females (5 per sex per group) at dose levels of 2,000, 2,500, 3,000, 3,500, 4,000 and 4,500 mg/kg bw. The rats were observed immediately after the administration of the test substance and then 1 h and 4 h after dosing and daily thereafter for 14 d. Body weights were recorded initially, Day 14 and at time of death. The surviving rats were sacrificed by CO2 inhalation and a gross necropsy was performed. None of the rats died at 2,000 and 2,500 mg/kg bw, two males died at dose level of 3,000 mg/kg bw, one male died at dose level of 3,500 mg/kg bw, two males and four females died at dose level of 4,000 mg/kg bw and three males and four females died at dose level of 4,500 mg/kg bw. Clinical signs observed were body drop, ptosis, decreased activity, labored respiration, chromodacryorrhea, decreased body tone, abnormal gait and stance, partial paralysis, loss of equilibrium, dried red exudate around nasal cavity, semiprostration, prostration, ataxia, diarrhea, hunched body position, poor grooming, shallow respiration, piloerection, clear discharge from eyes and lacrimation. Necropsy of dead animals showed distended, air filled stomachs and intestines, discolored adrenals, lungs, thymus, spleen, small ulcers or erosions of glandular mucosa, hemorrhages in cecum and small amounts of fluid in thoracic cavity. No visible lesions were observed in the remaining animals. Based on the results of the study, the acute oral LD50 of the test substance for male and female rats was determined to be ≥3,900 mg/kg bw, with 95% confidence limits of 3,514 to 4,329 mg/kg bw (Mallory VT, 1982).
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