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EC number: 258-964-5 | CAS number: 54079-53-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Justification for type of information:
- Experimental study comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: EU method C.1 (1992)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- A study was performed to assess the acute toxicity of test material to Danio rerio under static conditions.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Reconstituted water prepared according to the recommendations of ISO 7346. This freshly prepared standard dilution water was used for the maintenance of the test animals under flow-through conditions and for the preparation of stock- and test solutions of the test item.
- Details on test solutions:
- To produce the only test concentration 5.4 mg of the test item were added to 5 litres of dilution water, then treated with an ultrasonic bath for 60 min. and afterwards stirred for 24 h on a magnetic stirrer. Finally undissolved particles of the test item were removed by filtration using a folded filter of pore size 7-12 μm.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Source : Aqua KloeGer (Germany)
Acclimatisation : Stock held since 2007-09-18 and acclimatised to the test conditions since then.
Feeding : Commercial fish food, daily. Discontinued 24 h prior to test.
Mortalities during acclimatisation period : < 5 %
Mean standard length (n = 14) : 3.3 cm (S.D. = 0.15 cm) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure.
- Hardness:
- 11.1°dH (= 198 mg/L CaCO3).
- Test temperature:
- 21.4 to 22.3°C
- pH:
- 7.7
- Dissolved oxygen:
- 8.4 mg/l
- Nominal and measured concentrations:
- 1 mg/L
- Details on test conditions:
- Test vessels : glass aquaria holding 5 L of test media
Experimental design : 1 test concentration plus 1 control
7 animals per test concentration
no feeding during the exposure period
static system
Method of initiation : animals were placed in prepared media
Loading : 0.36 g body weight (wet weight) per litre
Photoperiod : 16 h light : 8 h dark
Photoperiod : 16 h light : 8 h dark
Temperature : 21.4 to 22.3°C
Aeration : gentle aeration via narrow glass tubes
Test item concentration/s : 1 mg/L
Method of administration : direct weighing
Medium renewal : none
Duration of exposure : 96 hours - Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No toxic effect at against fishes at the limit of water solubility under exposure conditions.
- Details on results:
- The criterion of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 2, 24, 48, 72 and 96 hours, type and incidence of sub-lethal effects compared with control fish were observed.
- Sublethal observations / clinical signs:
Table 1:
Control
Abiotic parameters
0 h
24 h
48 h
72 h
96 h
Temperature [°C]
21.4
21.5
21.7
21.6
21.6
Oxygen [mg/L]
9.3
8.5
8.4
8.5
8.4
Oxygen [% saturation]
105
95
97
97
96
pH value
7.7
7.7
7.7
7.8
7.7
Mortality for zebra fish
2 h
24 h
48 h
72 h
96 h
(initial population: 7)
Absolute
0
0
0
0
0
Cumulative
0
0
0
0
0
Cumulative [%]
0
0
0
0
0
Chemical analysis
0 h
24 h
48 h
72 h
96 h
HPLC replicates [mg/L]
<0.002/
<0.002/
<0.002/
<0.002/
<0.002/
<0.002
<0.002
<0.002
<0.002
<0.002
HPLC mean value [mg/L]
<0.002
<0.002
<0.002
<0.002
<0.002
Table 2:
1 mg/L
Abiotic parameters
0 h
24 h
48 h
72 h
96 h
Temperature [°C]
22.2
22.1
22.3
21.4
21.4
Oxygen [mg/L]
8.6
8.4
8.4
8.6
8.4
Oxygen [% saturation]
100
93
95
97
96
pH value
7.7
7.7
7.7
7.8
7.8
Mortality for zebra fish
2 h
24 h
48 h
72 h
96 h
(initial population: 7)
Absolute
0
0
0
0
0
Cumulative
0
0
0
0
0
Cumulative [%]
0
0
0
0
0
Chemical analysis
0 h
24 h
48 h
72 h
96 h
HPLC replicates [mg/L]
0.014
0.005
0.004
<0.002
<0.002
0.005
0.007
0.003
<0.002
0.002
HPLC mean value [mg/L]
0.010
0.006
0.004
<0.002
<0.002
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The median lethal effect concentration LC50 was observed to be >1 mg/l . No toxic effect at against fishes at the limit of water solubility under exposure conditions.
- Executive summary:
A study was performed to assess the acute toxicity of test material to Danio rerio under static conditions. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity in Directive 92/69/EEC (O.J. No. L383A, 1992) Part C, Method 1 'Acute toxicity for Fish' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992).
A group of seven fish of the recommended size was exposed to a limit test concentration of nominally 1 mg/L of Macrolex Yellow 6G dissolved in water. Auxiliaries used to prepare the test media were an ultrasonic bath, a magnetic stirrer and a folded filter. The water solubility of test material is very low i.e
< 0.0007 mg/L. Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. The median lethal effect concentration LC50 was observed to be >1 mg/l . No toxic effect at against fishes at the limit of water solubility under exposure conditions.
Reference
Description of key information
Short term toxicity to fish:
A study was performed to assess the acute toxicity of test material to Danio rerio under static conditions. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity in Directive 92/69/EEC (O.J. No. L383A, 1992) Part C, Method 1 'Acute toxicity for Fish' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992).
A group of seven fish of the recommended size was exposed to a limit test concentration of nominally 1 mg/L of Macrolex Yellow 6G dissolved in water. Auxiliaries used to prepare the test media were an ultrasonic bath, a magnetic stirrer and a folded filter. The water solubility of test material is very low i.e
< 0.0007 mg/L. Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. The median lethal effect concentration LC50 was observed to be >1 mg/l . No toxic effect at against fishes at the limit of water solubility under exposure conditions. The water solubility of test material is < 0.0007 mg/L , hence indicating that aquatic toxicity is unlikely to occure
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1 mg/L
Additional information
Short term toxicity to fish:
A study was performed to assess the acute toxicity of test material to Danio rerio under static conditions. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity in Directive 92/69/EEC (O.J. No. L383A, 1992) Part C, Method 1 'Acute toxicity for Fish' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992).
A group of seven fish of the recommended size was exposed to a limit test concentration of nominally 1 mg/L of Macrolex Yellow 6G dissolved in water. Auxiliaries used to prepare the test media were an ultrasonic bath, a magnetic stirrer and a folded filter. The water solubility of test material is very low i.e
< 0.0007 mg/L. Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. The median lethal effect concentration LC50 was observed to be >1 mg/l . No toxic effect at against fishes at the limit of water solubility under exposure conditions. The water solubility of test material is < 0.0007 mg/L , hence indicating that aquatic toxicity is unlikely to occure
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