Phosphonic acid, P-2-propen-1-yl-, diethyl ester

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 Nov 2014 to 18 Dec 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl allylphosphonate

EC Number:

619-646-5

Cas Number:

1067-87-4

Molecular formula:

C7H15O3P

IUPAC Name:

Diethyl allylphosphonate

Test material form:

other: liquid

Details on test material:

Identification: Diethyl Allylphosphonate
Appearance/Physical State: Clear colorless liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: at 0 and 72 h; in the pre-test at 0, 0.1, 1.0, 10 and 100 mg/L, in the final test at 0 and 100 mg/L (pooled samples)
- Sample storage conditions before analysis: at -20 °C

Test solutions

Vehicle:

no

Details on test solutions:

test substance was directly added to medium (preparation under laboratory safety lighting due to potential light sensitivity of the substance)

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Pseudokirchnerella subcapitata
- Strain: CCAP 278/4
- Source: SAMS Research Services Ltd, Scottish Marine Institute, Oban, Argyl, Scotland

ACCLIMATION
- Acclimation period: 100 mL master culture with 10E3 cells/mL was kept at 24±1 °C under constant agitation (100-150 rpm) and constant illumination until the total number of cells was 10E4-10E5/mL
- Cultivation medium: similar to test medium

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

24±1 °C

pH:

7.7-8.4

Nominal and measured concentrations:

pre-test: nominal 0, 0.1, 1.0, 10 and 100 mg/L; measured at 100 mg/L 93% and 87% of nominal at 0 and 72 h respectively
main test: nominal 0 and 100 mg/L; measured at 0 mg/L < LOQ at 0 and 72 h, at 100 mg/L 87 % and 96% of nominal at 0 and 72 h respectively

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL glass conical flasks
- Type: closed plugged with polyurethane foam
- Initial cells density: 5.8E03 cells/mL
- Control end cells density: 9.17E05 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes AAP according to guidance

TEST MEDIUM
- Source/preparation of dilution water: reversed osmosis purified deionized water
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Photoperiod: continuous
- Light intensity and quality: 7000 Lux
- Shaken: 150 rpm

EFFECT PARAMETERS MEASURED
- Determination of cell concentrations: final test at 0, 24, 48 and 72 h with Coulter Counter

TEST CONCENTRATIONS,
- Range finding study: 0, 0.1. 1.0, 10 and 100 mg/L
- Test concentrations: 0 and 100 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Results with reference substance (positive control):

potassium dichromate ErC50 1.2 mg/L, EyC50 0.63 mg/L

Reported statistics and error estimates:

No statistical differences between 100 mg/L and control group for groth rate and inhibition of biomass

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The ErC50 for the test substance in an algal groth inhibition test is > 100 mg/L

Executive summary:

Algae were exposed to 0 and 100 mg/L test substance during 72 hours. No effects on growth rate and biomass were found. The analyses for accuracy of preparations showed that the average measured concentrations were > 80% of nominal. Therefore the results are based on nominal concentrations.The ErC50 is > 100 mg/L

.