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EC number: 433-100-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-09-22 to 1999-12-07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- dated December 29, 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- adopted June 7, 1984.
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- A supersaturated stock suspension of the test substance with a nominal concentration of 100 mg/L was prepared by dosing 100 mg of the test substance into 1 L test water. No auxiliary solvent or emulsifier was used. The test substance was mixed into the test water as homogeneously as possible by ultrasonic treatment for 15 minutes and intense stirring. The supersaturated stock suspension was therefore stirred on a magnetic stirrer at room temperature in the dark over about 66 hours to dissolve, respectively disperse a maximum concentration of the test substance in test water.
The stock suspension of the test substance was filtered through a cellulose-nitrate filter with a defined pore size of 0.2 µm. The filtrate of the stock suspension with dissolved test substance was used as the highest concentrated test medium. Adequate volumes of the stock suspension filtrate were diluted with test water to prepare the test media of following dilutions: dilution 1:2, 1:4, 1:8 and 1:16. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: No. 86.81 SAG
- Source: The algae were supplied by the "Sammlung von Algenkulturen, Pflanzenphysiologisches Institut der Universität Göttingen", 37073 Göttingen, Germany - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- 24 mg CaCO3/L
- Test temperature:
- 23 - 24°C
- pH:
- 7.8 to 8.2 at test start
9.3 to 10.4 at test end - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks of 50 mL volume with 50 mL test medium
- Initial cells density: 10.000 algal cells per mL test medium
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6 - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 78.2 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 mg/L
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 mg/L
- Basis for effect:
- biomass
- Details on results:
- No concentrations of the dissolved components of the test substance (EC: 433-100-1) can be given, because no analytical method for dose verification is available. Therefore, the biological results of the algae test are based on the nominal concentration of 100 mg/L which was used to prepare the stock solution of the filtrate and to nominal concentrations of the test substance in the dilutions of the filtrate.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance (EC: 433-100-1) was tested for toxicity to aquatic freshwater algae in a 72 h-growth inhibition test according to EU method C.3 and OECD guideline 201. The ecotoxicity data indicates the test substance shows some toxicity to algae below the limit of its solubility.
- Executive summary:
- The test substance (EC: 433-100-1) was assessed in an aquatic toxicty test to algae according EU-method C.3 and OECD guideline 201. Due to the low water solubility limit of the test substance a filtrate of a supersaturated stock suspension of nominal 100 mg/L was prepared. The filtrate of the stock suspension with dissolved test substance was used as the highest concentrated test medium. Adequate volumes of the stock suspension filtrate were diluted with test water to prepare the test media of following dilutions: dilution 1:2, 1:4, 1:8 and 1:16. Additionally, a control was tested in parallel. The 72-hour NOEC for the growth rate and for biomass was determined to be nominal 50 mg/L (1:2 dilution). The 72- hour EC50 for the growth rate was calculated to be >100 mg/L and for biomass 78.2 mg/L based on nominal concentrations.
Reference
Description of key information
The test substance (EC: 433-100-1) was assessed in an aquatic toxicity test to algae according EU-method C.3 and OECD guideline 201. Due to the low water solubility limit of the test substance a filtrate of a supersaturated stock suspension of nominal 100 mg/L was prepared. The filtrate of the stock suspension with dissolved test substance was used as the highest concentrated test medium. Adequate volumes of the stock suspension filtrate were diluted with test water to prepare the test media of following dilutions: dilution 1:2, 1:4, 1:8 and 1:16. Additionally, a control was tested in parallel. The 72-hour NOEC for the growth rate and for biomass was determined to be nominal 50 mg/L (1:2 dilution). The 72- hour EC50 for the growth rate was calculated to be >100 mg/L and for biomass 78.2 mg/L based on nominal concentrations.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
Additional information
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