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EC number: 200-824-2 | CAS number: 74-95-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted prior to availability of the appropriate OECD TG and OPPTS testing guidelines; however, it is well documented and is scientifically acceptable. Hence, it is rated klimisch 2 and was identified as a key study and .
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
Materials and methods
Test guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- Rats were exposed to the vapor concentrations of the test material in a dynamic air-flow inhalation exposure system for 4 hours.
- GLP compliance:
- no
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- Dibromomethane
- EC Number:
- 200-824-2
- EC Name:
- Dibromomethane
- Cas Number:
- 74-95-3
- Molecular formula:
- CH2Br2
- IUPAC Name:
- dibromomethane
- Details on test material:
- unspecified purity, DBM
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: albino CD
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- Humidity, temperature, flow-rate and static pressure were measured and recorded.
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Details on inhalation exposure:
- in a dynamic air-flow inhalation exposure system for 4 hours
- Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- using FIDAMAT, Siemens A.G., Germany
- Duration of exposure:
- 4 h
- Concentrations:
- 22.3, 22.2, 22.1, 21.7 and 21.4 mg/m3
- No. of animals per sex per dose:
- Five groups of 12 male
- Control animals:
- yes
- Details on study design:
- At termination of exposure, rats were immediately removed from their individual chambers and observed for any specific symptoms and/or changes in behavior.
- Statistics:
- Statistical analysis was limited to calculation of means and standard deviations of body weight and body weight change.
Results and discussion
- Preliminary study:
- n/a
- Mortality:
- none
- Clinical signs:
- other: Animals seemed seriously incapacitated in their spontaneous motor activity as exhibited by slow breathing, uncoordinated and atactic walk, continuous tremor, and excessive preening. All these effects wore off within about 3 hours when most of the exposed
- Body weight:
- Immediately upon removal from the exposure chambers, animals lost weight of ~5.4g apparently due to excessive fluid excretion.
- Gross pathology:
- No gross necropsy findings were observed
- Other findings:
- No further information
Any other information on results incl. tables
No further information
Applicant's summary and conclusion
- Conclusions:
- In the 4 hours inhalation study in rats receiving 22.3, 22.2, 22.1, 21.7 and 21.4 mg/m3 DBM in a dynamic air-flow inhalation exposure system, no mortalities had occurred and no lasting pathological changes were seen at 18 and 20 days post treatment. Based on the results LC50> 22.3mg/m3 can be concluded.
- Executive summary:
Five groups of 12 male albino CD rats were exposed to the following vapor concentrations of the test material (unspecified purity, DBM): 22.3, 22.2, 22.1, 21.7 and 21.4 mg/m3 in a dynamic air-flow inhalation exposure system for 4 hours. Humidity, temperature, flow-rate and static pressure were measured and recorded. At termination of exposure, rats were immediately removed from their individual chambers and observed for any specific symptoms and/or changes in behavior.
Immediately upon removal from the exposure chambers, animals lost weight of ~5.4g apparently due to excessive fluid excretion. They seemed seriously incapacitated in their spontaneous motor activity as exhibited by slow breathing, uncoordinated and atactic walk, continuous tremor, and excessive preening. All these effects wore off within about 3 hours when most of the exposed animals were found in deep sleep. On the following morning, the treated rats seemed exactly like the control ones. At the end of 18 to 20 days observation period, animals were autopsied. No obvious pathological differences in internal organs could be detected between experimental and control animals. Based on the outcome of this study, LC50> 22.3mg/m3 can be concluded.
The study was well documented and is scientifically acceptable. Hence, it was identified as a key study and was graded klimisch 2.
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