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Diss Factsheets
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EC number: 207-431-5 | CAS number: 470-82-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- No data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards with acceptable restrictions.
Data source
Reference
- Reference Type:
- publication
- Title:
- Biotransformation of 1,8-cineole by human liver microsomes.
- Author:
- Miyazawa M & Shindo M.
- Year:
- 2 001
- Bibliographic source:
- Natural Product Letters 15 (1) 49 - 53.
Materials and methods
- Objective of study:
- metabolism
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The biotransformation of 1,8-cineole was investigated using human liver microsomes.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1,8-cineole
- IUPAC Name:
- 1,8-cineole
- Test material form:
- not specified
- Details on test material:
- - Name of test material (as cited in study report): 1,8-cineole
Constituent 1
- Radiolabelling:
- not specified
Test animals
- Species:
- human
- Strain:
- other: Not applicable
- Sex:
- not specified
Administration / exposure
- Route of administration:
- other:
- Vehicle:
- not specified
- Duration and frequency of treatment / exposure:
- 30 minutes
Doses / concentrations
- Remarks:
- Doses / Concentrations:
200 µM
- No. of animals per sex per dose / concentration:
- Not applicable
- Control animals:
- not specified
- Details on study design:
- A standard reaction mixture containing human liver microsomes (0.025 mg/mL) with the test substance was incubated in a final volume of 0.50 mL of 100 mM potassium phosphate buffer (pH 7.4) containing NADPH. Incubtations were carried out at 37 °C for 30 minutes and terminated by adding ethyl acetate. The mixtures were stirred vigorously and the extracts (organic layer) were collected by centrifugation at 3000 rpm for 10 min. The organic phase was transferred to an insert for analysis by GC-MS.
Results and discussion
Metabolite characterisation studies
- Metabolites identified:
- yes
- Details on metabolites:
- The formation of the 2-exo-hydroxylated metabolite was suggested by the GC chromatogram. The molecular weight of the metabolite increased from 154 to 170 by introduction of oxygen atom. A dehydration peak changed from 126 to 108 indicting that a hydroxyl group was introduced into the molecule. The position and relative stereochemistry of a hydroxyl group were determined by relative abundance of mass fragments and retention time of GC. Their values were consistent with those of authentic sample which 2-exo-hydroxy-1,8-cineole was obtained from biotransformation of 1,8-cineole by microorganism. From the above results, the metabolite was established as 2-exo-hydroxy-1,8-cineole.
The incubation time, P450 contents and the 1,8-cineole concentration in the metabolism of 1,8-cineole by liver microsomes was investigated. On incubation with human liver microsomes in the presence of NADPH, the metabolite was found to be formed with the increase of incubation time, P450 levels and substrate concentration. 1,8-cineole 2-hydroxylation activity was shown to be high for a short incubation time, a small quantity of P450 levels in liver microsomes at low concentration. Therefore, 1,8-cineole 2-hydroxylation cataysed by human liver microsomes was found to be efficiently conducted.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): bioaccumulation potential cannot be judged based on study results
The biotransformation of 1,8-cineole was investigated using human liver microsomes. The metabolite was established as 2-exo-hydroxy-1,8-cineole. 1,8-cineole 2-hydroxylation cataysed by human liver microsomes was found to be efficiently conducted.
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