N-(N6-trifluoroacetyl-L-lysyl)-L-proline

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996-01-10 to 1996-04-19

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: During the test duplicate samples were taken from 0 and 100 mg/L for analysis.
- Sampling method: Sampling: Frequency at t=0h (extra sample at 100 mg/L) and t=48h. Volume 10 mL from the approximate centre of the test vessel.
- Sample storage conditions before analysis: Samples not analyzed on the day of sampling were stored in a deep freeze. Specification of the samples analysed and the method of analysis are described in the appended Analytical Report.

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solutions were prepared using a stock solution of 100 mg/L by adding quantitatively 25.1 mg of test substance to 250 mL ISO-medium. The final test solution was all clear without precipitation.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: Daphnia magna (Crustacea, Cladocera) (Straus, 1820)
- Age at study initiation (mean and range, SD): < 24h
- Method of breeding: Start of each batch with newborn animals, i.e. less than 3 days old, by placing about 250 of them into 10 litres of medium in an all-glass culture vessel. Maximum age of the cultures 4 weeks. Renewal of the cultures. After 7 days of cultivation half of the medium twice a week.
- Feeding during test None

ACCLIMATION
- Acclimation period: n/a inhouse culture
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: a suspension of fresh water algae.
- Feeding frequency: daily
- Health during acclimation (any mortality observed): not reported

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

250 mg/L CaCO3

Test temperature:

between 20.0 and 20.5 °C

pH:

between 7.8 and 8.4

Dissolved oxygen:

between 8.8 and 9.3 mg/L

Nominal and measured concentrations:

Nominal concentrations: 0, 0.1, 1.0, 10, 100, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass vessels
- Material, size, headspace, fill volume: glass, volume: 100 mL, fill volume: 80 mL
- Aeration: No
- No. of organisms per vessel: 20 in the controls and at 100 mg/L; 10 at 0.1, 1, and 10 mg/L
- No. of vessels per concentration (replicates): duplicate
- No. of vessels per control (replicates): duplicate

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4, as prescribed by Dr. Elendt-Schneider (Elendt, B.-P., 1990: Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus. Protoplasma 154, 25-33).
- Culture medium different from test medium: No

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16/8
- Light intensity: not reported

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

VEHICLE CONTROL PERFORMED: yes/no

RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

In a test conducted according to OECD guideline 202 (1984), daphnids were treated with the test substance up to a concentration of 1000 mg/L for 48h. The 48h EC50 is considered to be > 1000 mg/L (nominal concentration).

Description of key information

In a test conducted according to OECD guideline 202 (1984), daphnids were treated with ε-trifluoracetyl-L-lysyl-L-proline (TFA-Lys-Pro) up to a concentration of 1000 mg/L for 48h. The 48h EC50 is considered to be > 1000 mg/L (nominal concentration).