Registration Dossier
Registration Dossier
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EC number: 500-004-7 | CAS number: 9003-29-6
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Oct 2007 - Dec 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Butene, homopolymer (products derived from either/or But-1-ene/But-2-ene)
- EC Number:
- 500-004-7
- EC Name:
- Butene, homopolymer (products derived from either/or But-1-ene/But-2-ene)
- Cas Number:
- 9003-29-6
- Molecular formula:
- (C4 H8)x
- IUPAC Name:
- Butene, homopolymer (products derived from butene)
- Details on test material:
- - Batch number: from continous production
- Aggregate state: liquid
- Colour: colourless, clear
- Purity of the test substance: > 99.9 area-%
- Homogeneity: homogenous
- TOC: 855 mg/g
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge from wastewater treatment plant treating municipal sewage, Mannheim/Baden-Wuerttemberg, Germany
- Concentration of sludge: 30 mg/l dry substance - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 12 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22 +/- 2 °C
- pH: 7.2 - 7.4
TEST SYSTEM
- Number of culture flasks/concentration: 2
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: yes
- Details of trap for CO2: The produced CO2 was trapped in an absorptive solution (0.05 mol sodium hydroxide). The Total Inorganic Carbon values of the adsorption solutions were determined twice a week and used for calculation of the produced carbon dioxide.
SAMPLING
- Sampling frequency: every 2-4 days for at least 28 days
CONTROL AND BLANK SYSTEM
- Inoculum blank
- Inhibition control
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 10 - 20
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- 1% after 1 day
80% after 14 days
83% after 28 days
Applicant's summary and conclusion
- Interpretation of results:
- other: poorly biodegradable
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