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EC number: 221-259-8 | CAS number: 3048-64-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- Guideline study with some deviations compared to current standards. Original study report not available.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 998
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 475 (Mammalian Bone Marrow Chromosome Aberration Test)
- Deviations:
- yes
- Remarks:
- . Only half prescribed number of cells scored per animal for mitotic index and analysed for abberations.
- GLP compliance:
- not specified
- Type of assay:
- chromosome aberration assay
Test material
- Reference substance name:
- 5-vinylnorborn-2-ene
- EC Number:
- 221-259-8
- EC Name:
- 5-vinylnorborn-2-ene
- Cas Number:
- 3048-64-4
- Molecular formula:
- C9H12
- IUPAC Name:
- 5-ethenylbicyclo[2.2.1]hept-2-ene
- Test material form:
- liquid
- Details on test material:
- - Test substance: 5-vinyl-2-norbornene (CAS 3048-64-4)
- Purity: 98.44%
- Source: Union Carbide Corporation, South Charleston, West Virginia
- LOT 59FGC27
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: M: 219.6 - 270g, F:145.7-176.4g
- Housing: singly in stainless steel wire mesh cages.
- Diet and water: ad libitum except during exposure period
- Acclimation period: 3 weeks
ENVIRONMENTAL CONDITIONS
- Temperature (°F): 66-77
- Humidity (%): 40-70
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- inhalation: vapour
- Details on exposure:
- TYPE OF INHALATION EXPOSURE: whole body
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: 900l stainless steel chamber with glass window.
- Air flow rate: 200l/min
- Air change rate: 13-14/hr
TEST ATMOSPHERE
- Vapour generated by liquid metering piston pump feeding into a heated cylindrical glass column evaporator at 43-4-47.6degC.
- Brief description of analytical method used: Sampled every 30 mins, analysis by GC-FID. LOD=2ppm.
- Samples taken from breathing zone: no - Duration of treatment / exposure:
- 5 days
- Frequency of treatment:
- 6hr/day
- Post exposure period:
- Half of exposed groups (5 males, 5 females per dose group): 24 hours.
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
0, 50, 150, 350ppm
Basis:
other: target concentration
- Remarks:
- Doses / Concentrations:
0, 48.1±1.29, 146±9.2, 336±8.5ppm
Basis:
analytical conc.
- No. of animals per sex per dose:
- 10 (5 for positive controls)
- Control animals:
- yes, sham-exposed
- Positive control(s):
- cyclophosphamide
- Route of administration: ip
- Doses / concentrations: 30mg/kg
Examinations
- Tissues and cell types examined:
- Femoral bone marrow.
- Details of tissue and slide preparation:
- DETAILS OF SLIDE PREPARATION: Femoral bone marrow flushed into a centrifuge tube using 10–15 ml of freshly prepared Hank’s balanced salt solution (pH 7.0). The suspension was centrifuged and the pellet resuspended in 0.075 M KCl and incubated at 37°C for 20–30 min. Cells were centrifuged and fixed with two or three changes of 3:1 methanol–acetic acid. Fixed cells were refrigerated at 4°C for 12 h before slide preparation. Cells were then centrifuged and resuspended in fixative. The suspension was dropped onto a clean wet slide, air dried, stained with a dilute Giemsa solution, rinsed with water, air dried again and then a coverslip was applied.
Analysis of the two exposure groups was effectively at 6 and 24hrs post exposure.
METHOD OF ANALYSIS: 500 cells per animal scored to determine a mitotic index, and 50 metaphase cells per animal evaluated for frequency and type of chromosomal damage. Each cell was evaluated for chromosome number, specific chromosome or chromatid aberration or exchanges. - Statistics:
- Mann–Whitney U-test to evaluate chromosome abberations
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- A statistically significant weight decrease was seen for the 6hr 50ppm males sampling group but since no effects were seen at higher concentrations the effect was deemed not biologically significant.
Applicant's summary and conclusion
- Conclusions:
- Negative
- Executive summary:
In an in vivo chromosome abberation inhalation study using Sprague-Dawley rats, 5 -vinyl-2 -norbornene showed no evidence of clastogenic effects at any tested concentration.
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