Cyclohexanecarboxylic acid, 1-[[2-(2,5-dimethylphenyl)acetyl]amino]-4-methoxy-, methyl ester, cis-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

adopted July, 1992

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

methyl cis-1-{[(2,5-dimethylphenyl)acetyl]amino}-4-methoxycyclohexanecarboxylate

EC Number:

606-524-1

Cas Number:

203313-47-7

Molecular formula:

C19H27NO4

IUPAC Name:

methyl cis-1-{[(2,5-dimethylphenyl)acetyl]amino}-4-methoxycyclohexanecarboxylate

Test material form:

solid: bulk

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Aeration tank of a wastewater plant treating predominantly domestic sewage
(Wupper area water authority, STP Odenthal)
- Laboratory culture: the sludge was washed twice by adding mineral medium and centrifuging
for 10 min at 2000 rpm and 20 °C and decanting off the supernatant
- Storage conditions: The inoculum was stored at room temperature under continuous shaking
with aeration until use for max. 3 days

Initial test substance concentration

Initial conc.:

100 mg/L

Details on study design:

TEST CONDITIONS
- Test temperature: 22°C
- pH: 7.4 - 7.9
- pH adjusted: no

TEST SYSTEM
- Culturing apparatus: Oxitop
- Number of culture flasks/concentration: 2
- Test performed in closed vessels due to significant volatility of test substance:

CONTROL AND BLANK SYSTEM
- Inoculum blank: 3
- Abiotic sterile control: -
- Toxicity control: 1

Pre-treatment of the test item
25 mg of the test item were weighed out on aluminium foil. Test item and
aluminium foil were added to the test flasks, which had already been filled with 200
mL of mineral medium. Afterwards the flask content was made up to 250 mL with
mineral medium containing inoculum to give a test concentration of 100 mg test
item/L.

Exposure conditions
Test volume: 250 mL
Test apparatus: OxiTop System (WTW)
Mixing: 1 magnetic stirrer per test vessel
Incubation time: 28 days
Incubation temperature: 22±1 °C

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

1.4

Sampling time:

7 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

0.4

Sampling time:

14 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

21 d

% Degradation 4

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

82.0 % in 14 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Remarks:

Within 28 days, a degradation o 0 % was determined.

Conclusions:

1.0 % degradation after 7 days
0.4 % degradation after 14 days
0 % degradation after 21 days
0 % degradation after 28 days
Therefore, the test subsance is considered to be “Not Readily Biodegradable“.

Executive summary:

The study was conducted in accordance with the Council Directive 92/69/EEC Method C.4-D “Manometric Respirometry Test“(1992). This test method is in all essential parts identical with OECD Guideline 301 F (adopted July 1992).

Within 28 days, a degradation of 0 % was determined for the test substance.

The test substance is considered to be "Not Readily Biodegradable".