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EC number: 282-817-4 | CAS number: 84434-18-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-ethyl-N-methyl-N-(3-methylphenyl) butanamide
- EC Number:
- 446-190-2
- EC Name:
- 2-ethyl-N-methyl-N-(3-methylphenyl) butanamide
- IUPAC Name:
- 446-190-2
- Reference substance name:
- 406488-30-0
- Cas Number:
- 406488-30-0
- IUPAC Name:
- 406488-30-0
- Test material form:
- other: liquid
- Details on test material:
- Sponsor's identification: M 019087
Description: clear colourless liquid
Batch No.: 01/02
Date received: 30 May 2002
Storage conditions: approximately 4 °C, in the dark
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The test samples were analysed following filtration through glass wool in order to remove the algal cells. A volume of the saturated solution sample was diluted with test media to give a final theoretical concentration of 32 mg/L.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- A modification of the standard method for the preparation of aqueous media was performed. An approach endorsed by several important regulatory authorities in the EU and elsewhere (ECETOC 1996 and OECD 2000), is to expose organisms to a saturated solution of the test material in cases where the test material is of high purity and is poorly soluble in water.
The test material solutions were prepared by shaking an excess (2000mg/l) of test material in culture medium at 300 rpm at a temperature of 30'C for 24 hours prior to removing any undissolved test material by filtration through a pre-conditioned filter to produce a saturated solution. This saturated solution was then further diluted as necessary to prepare the required test concentrations.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- The test was carried out using Scenedesmus subspicatus strain CCAP 276/20. Liquid cultures of Scenedesmus subspicatus were obtained from the Culture Collection of Algae and Protozoa (CCAP), Insititue of Freshwater Ecology, The Ferry House, Far Sawrey, Cumbria. Cultures were maintained in the laboratory by the periodic replenishment of culture medium. The culture was maintained in the laboratory at a temperature of 21 ± 1 °C under continuous illumination (intensity approximately 7000 lux) and constant aeration.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None
Test conditions
- Test temperature:
- The flasks were incubated at 24 ± 1°C
- pH:
- The pH of each control and test flask was determined at initiation of the test and after 72 hours exposure. The pH was measured using a WTW pH 320 pH meter. The pH values of the control cultures were observed to increase from pH 7.1 at 0 h to pH 8.4 at 72 h. The pH deviation in the control cultures was less than 1.5 pH units after 72 hours and therefore within the limits given in the test guidelines.
The test material vessels showed an increase in pH over the 72-hour test period following a concentration dependent pattern with the lower test material concentrations exhibiting a greater increase in pH. This effect was considered to be due to there being greater numbers of viable cells in the lower test concentration and hence utilisation of carbonates and bicarbonates from photosynthesis/respiration. - Nominal and measured concentrations:
- Nominal concentrations:
Range-finding test: 0.1, 1.0, 10 and 100 mg/L
Definitive test: 6.25, 12.5, 25, 50 and 100
Chemical analysis of the solutions at 0 and 72 hours showed measured concentrations to be in the range of 85 to 92 % of nominal and so the results are based on nominal test concentrations only. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 30 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 27-33
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL: 23 - 29 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and biomass
- Details on results:
- Chemical analysis of the solutions at 0 and 72 hours showed measured concentrations to be in the range of 85 to 92 % of nominal and so the results are based on nominal test concentrations only.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a guideline study, conducted according to GLP, Paradisamide was found to have an EbC50 (72 hr) of 26 mg/L and an ErC50 (72 hr) of 30 mg/L. The No Observed Effect Concentration at 72 hours was 12.5 mg/L.
The preferred observational end point in this study is algal growth rate inhibition because it is not dependent on the test design (ECHA guidance Chapter R.7b v1.1). The EU CLP regulation (No 1272/2008 and its adaption 286/2011) also states that classification should be based on the ErC50. Thus the 72-h EC50 and NOEC based on growth rate are used for classification purposes - Executive summary:
Paradisamide was evaluated for its inhibition to algae in a study conducted according to OECD Guideline 201 and EU method C.3 of EC Directive 92/69/EEC (1992), and performed to GLP. Paradisamide was tested at nominal concentrations of 6.25, 12.5, 25, 50 and 100 mg/L for 72 hr in a static system. Paradisamide was found to have an EbC50 (72 hr) of 26 mg/L and an ErC50 (72 hr) of 30 mg/L. The No Observed Effect Concentration at 72 hours was 12.5 mg/L.
Chemical analysis of the solutions at 0 and 72 hours showed measured concentrations to be in the range of 85 to 92 % of nominal and so the results are based on nominal test concentrations only.
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