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EC number: -
CAS number: -
The purpose of the study was to evaluate the inhibition effect of the
test substance on seed germination and root elongation toxicity of the
test species after the seeds were exposed to a series of concentrations
of the test substance over the test period and develop data on the
phytotoxicity of the test substance.
Test seeds were exposed to a series of test concentrations (5 mg/L, 10
mg/L, 50 mg/L, 100 mg/L, 500 mg/L and 1000 mg/L) of the test substance.
The test period ended when at least 65% of the control seeds have
germinated and developed roots at least 20 mm long. When both conditions
were met, the mean number of seed germinating and mean root length per
treatment and control were determined, and the inhibition effect of the
test substance was evaluated.
A volume for each test concentration of the prepared test stock
solution, with a concentration of 1000 mg/L test substance, was mixed
with quartz sand, for two replicates, and placed into a ventilation
cabinet to evaporate the cosolvent (THF) overnight. Once the THF had
evaporated completely, the percentage recovery of the test substance was
determine as 105% and 102%, which revealed that the test substance had
no obvious loss during volatilisation of THF.
The cosolvent control showed no significant difference of germination
and root length in the blank and cosolvent control.
Under the present conditions of the study, the EC50 of germination and
root elongation inhibition rates of the 10 seeds were all greater than
1000 mg/L. The inhibition effect of the test substance to seeds was not
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