Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: -
CAS number: -
The study was performed to assess the ready biodegradability of the test
material in an aerobic aqueous medium. The test material, at a
concentration of 10 mg Carbon/l, was exposed to activated sewage sludge
micro-organisms with culture medium in sealed culture vessels in the
dark at approximately 21 degrees Celsius for 42 days. At the request of
the Sponsor the study was extended from 28 days to 42 days. To aid
dispersion in the test medium and increase the surface area exposed to
the test organisms, the test material was dissolved in solvent prior to
being adsorbed onto the granular silica gel. Two
different series of vessels were prepared using silica gel alone and the
other using silica gel with the addition of a surfactant (Tween® 85
(Polysorbate 85)). The addition of a surfactant aids in solubilizing the
test material in the test media. The degradation of the test material
was assessed by the determination of carbon dioxide produced. Control
solutions with inoculum and surfactant, where applicable, and the
standard material, sodium benzoate, together with a toxicity control
were used for validation purposes.
Where no surfactant was present the test material attained 51%
degradation after 28 days and a final degradation of 67% after 42 days.
In the presence of the surfactant, the test material attained 50%
degradation after 28 days and a final 72% degradation after 42 days.
With and without surfactant, the test did not meet the validity criteria
- reaching 60% threshold degradation within 10 days of meeting 10%
degradation, within 28 days from test initiation. Under the strict terms
and conditions of OECD Guideline No 301B the test material cannot be
considered to be readily biodegradable as the test material failed to
meet the validation criterion. However, the test material has exhibited
A key GLP study conducted in accordance with the OECD 301B guideline, biodegradation in water- rapidly biodegradable but failing the 10-day window
A key GLP study was conducted in accordance with the OECD 301B guideline
to assess the ready biodegradability of the test material in an aerobic
aqueous medium. The test material was dissolved in solvent prior to
being adsorbed onto granular silica gel prior to dispersion in the test
medium to aid dispersion of the test material in the test medium and to
increase the surface area of the test material exposed to the test
Where no surfactant was present the test material attained 67%
degradation after 42 days (63 % degradation after 24 days). In the
presence of the surfactant, the test material attained 72% degradation
after 42 days. The OECD guideline criteria for classification of a
chemical as readily biodegradable are as follows: 1) percent
biodegradation must reach 60% within 28 days, and 2) the 60% degradation
must be attained within 10 days of exceeding 10% biodegradation.
Under the strict terms and conditions of OECD Guideline No 301B the test
material was considered to be rapidly biodegradable but it failed to
satisfy the 10-Day window validation criterion.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again