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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From October 21st, 2002 to November 05th, 2002
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1992
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
1992
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Yellow 6314-PPT
IUPAC Name:
Yellow 6314-PPT
Test material form:
solid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Due to the low water solubility of the test item, a supersaturated dispersion of the test item with a loading rate of 100 mg/l was continuously stirred at room temperature in the dark for 3 hours. Then the dispersion was filtered. Only the undiluted filtrate with the maximum concentration of dissolved test item was used as test medium. Additionally, a control was tested in parallel.

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
7 fishes tested per control
7 fishes tested per undiluted filtrate

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
250 mg CaCO3/l
Test temperature:
14 °C
pH:
7.9
Dissolved oxygen:
8.7 - 9.2 mg/l control
8.7 - 8.9 mg/l undiluted filtrate
Nominal and measured concentrations:
Nominal 100 mg/l as loading rate
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
ln the control and in the undiluted filtrate (loading rate of 100 mg/l) no mortality or other visible abnormalities were determined during the test period of 96 hours. Therefore, the 96-hour NOEC and the 96-hour LC0 of the test item to rainbow trout were determined to be at the loading rate of 100 mg/l. The 96-hour NOEC and the 96-hour LC0 might even be higher, but loading rates in excess of 100 mg/l have not been tested, due to the low water solubility of the test item in the used test water.

Applicant's summary and conclusion

Conclusions:
The test item had no toxic effects on rainbow trout up to its solubility limit in test water.